Lifesaving scientific tools made of paper

So, I love making tools

and sharing them with people.

I remember as a child,

my first tool I built
was actually a microscope

that I built by stealing lenses
from my brother's eyeglasses.

He wasn't that thrilled.

But maybe because of that momement,

30 years later,

I'm still making microscopes.

And the reason I built these tools
is for moments like this.

(Video) I have black things in my hair --

MP: This is a school in [the Bay Area.]

The living world far supersedes
our imagination

of how things actually work.

(Video) Oh my God!

MP: Right ...

"Oh my God!"

I hadn't realized this would be
such a universal phrase.

Over the last two years,

in my lab,

we built 50,000 Foldscopes,

and shipped them to 130
countries in the world

at no cost to the kids we sent them to.

This year alone,

with the support of our community,

we are planning to ship one million
microscopes to kids around the world.

What does that do?

It creates an inspiring community
of people around the world,

learning and teaching each other,

from Kenya to Kampala
to Kathmandu to Kansas.

And one of the phenomenal things
that I love about this

is the sense of community.

There is a kid in Nicaragua

teaching others how to identify
mosquito species that carry dengue

by looking at the larvae
under a microscope.

There is a pharmacologist
who came up with a new way

to detect fake drugs anywhere.

There is a girl who wondered,

how does glitter actually work?

And discovered the physics
of crystal information in glitter.

There is an Argentinian doctor

who's trying to do field cervical cancer
screening with this tool.

And yours very truly found
a species of flea

that was dug inside my heel in my foot
one-centimenter deep.

Now, you might think
of these as anomalies,

but there is a method to this madness.

I call this frugal science.

The idea of sharing experience of science
and not just the information.

Remind you,

there are a billion people on this planet

who live with absolutely
no infrastructure:

no roads,

no electricity,

and thus, no health care.

Also there a billion kids
on this planet that live in poverty.

How are we supposed to inspire them

for the next generation
of solution makers?

There are health care workers
that we put on the line

to fight for infectious diseases,

to protect us with absolutely
bare-minimum tools and resources.

So as a lab at Stanford,

I think of this from a context
of frugal sciecnce

and building solutions
for these communities.

Often we think about being able to do
diagnosis under a tree, off-grid.

I'll tell you two examples
today of new tools.

One of them starts in Uganda.

In 2013,

on a fieldtrip to detect
schistosomiasis with Foldscopes,

I made a minor observation.

In a clinic,

in a far, remote area,

I saw a centrifuge
being used as a doorstop.

I mean quite literally the doorstop.

And I asked them and they said,

"Oh, we don't actually have electricity,

so this piece of junk
is good as a doorstop."

Centrifuges,

for some of you who don't know,

are the pinnacle tool to be able
to do sample processing.

You separate components
of blood or body fluids

to be able to detect
and identify pathogens.

But centrifuges are bulky, expensive --

costs around 1,000 dollars --

and really hard to carry out in the field,

and of course, they don't
work without power.

Sound familiar?

So we started thinking
about solving this problem,

and I came back --

kept thinking about toys.

Now ...

I have a few with me here.

I first started with yo-yos ...

and I'm a terrible yo-yo thrower.

Because these objects spin,

we wondered,

could we actually use
the physics of these objects

to be able to build centrifuges?

This was possibly the worst
throw I could make.

But you might start realizing,

if you start exploring
the [safe space] of toys --

we tried these spinning tops,

and then in the lab,

we stumbled upon this wonder.

It's the Whirligig,

or a Buzzer,

or a [Roudrun.]

A couple of strings and a little disk,

and if I push, it spins.

How many of you have played
with this as a kid?

This is called a button-on-a-string.

OK, maybe 50 percent of you.

What you didn't realize --

that this little object

is the oldest toy
in the history of mankind.

5,000 years ago ---

we have found relics of this object
hidden around on our planet.

Now the irony is we actually
don't understand

how this little thing works.

That's when I get excited.

So we got back to work,

wrote down a couple of equations.

If you take the input torque
that you put in,

you take the drag on this disc,

and the twist drag on these strings,

you should be able
to mathematically solve this.

This is not the only equation in my talk.

10 pages of math later,

we could actually write down
the complete analytical solution

for this dynamic system.

And out comes what we call Paperfuge.

That's my postdoc,

Saad Bhamla,

who's the co-inventor of Paperfuge.

And to the left,

you see all the centrifuges
that we are trying to replace.

This little object that you see right here

is a disc, a couple
of strings and a handle.

And then I spin.

And I push,

it starts to spin.

Now, when you realize,

when you do the math,

when we calculate the rpm for this object,

mathematically we should be able
to go all the way to a million rpm.

Now there is a little twist
in human anatomy,

because the resident frequency
of this object is about 10 hertz,

and if you ever played piano,

you can't go higher
than two or three hertz.

The maximum speed we've been
able to achieve with this object

is not 10,000 rpm,

not 50,000 rpm --

120,000 rpm.

That's equal to 30,000 g-forces.

If I was to stick you right here
and have it spin,

you would think about the types
of forces you would experience.

One of the factors of a tool like this
is to be able to do diagnosis with this.

So I'm going to do a quick demo here.

So this is a moment where I'm going
to make a little fingerprint.

A tiny drop of blood is going to come out,

if you don't like blood,

you don't have to look at it.

Here is a little lancet.

These lancets are available everywhere,

completely passive.

And if I've had breakfast today ...

That didn't hurt at all.

OK, I take a little capillary
with a drop of blood --

now this drop of blood has answers,

that's why I'm interested in it.

It might actually tell me whether
I have malaria right now or not.

I take a little capillary,

and you see it starts leaking in.

I'm going to draw a little more blood.

And that's good enough for right now.

Now, I just seal this capillary
by putting it in clay.

And now that's sealed the sample.

We're going to take the sample,

mount it on Paperfuge.

A little piece of tape
to make a sealed cavity.

So now the sample is completely enclosed.

And we are ready for a spin.

I'm pushing and pulling with this object.

I'm going to load this up ...

and you see the object starts spinning.

Unlike a regular centrifuge,

this is a counter-rotating centrifuge.

It goes back and forth, back and forth,

and I'm charging it up --

and you see it builds momentum.

And now --

I don't know if you can hear this --

30 seconds of this

and I should be able to separate
all the blood cells with the plasma.

And the ratio of those
blood cells to plasma --

(Applause)

Already, if you see right here,

if you focus on this,

you should be able to see
a separated volume

of blood and plasma.

And the ratio of that actually tells me
whether I might be anemic.

One the of aspects of this is we build
many types of Paperfuges.

This one allows us to identify
malaria parasites

by running them for a little longer,

and we can identify malaria parasites
that are in the blood

that we can separate out and detect
with something like a centrifuge.

Another version of this allows me
to separate nucleic acids

to be able to do nucleic acid tests
out in the field itself.

Here is another version that allows me
to separate bulk samples,

and then finally,

something new that we've been working on

to be able to implement the entire
multiplex test on an object like this.

So where you do the sample preparation
and the chemistry in the same object.

Now ...

this is all good,

but when you start thinking about this,

you have to share these tools with people,

and one of the things that we did
is we just got back from Madagascar --

this is what clinical trials
for malaria look like --

(Laughter)

You can do this while having coffee.

But most importantly,

this is a village six hours from any road.

We are in a room with one of the senior
members of the community

and a health care worker.

And it really is this portion of the work
that really excites me the most --

that smile.

To be able to share simple but powerful
tools with people around the world.

Now, I forgot to tell you this --

that all of that cost me 20 cents to make.

OK, in the negative time I have left,

I'll tell you about the most recent --

(Laughter)

invention from our lab.

It's called Abuzz.

The idea that all of you
could help us fight mosquitoes.

You could all help us track our enemies.

These are enemies because they cause
malaria, Zika, chikagunya, dengue,

but the challenge is that we actually
don't know where our enemies are.

The world map for where
mosquitoes are is misssing.

So we started thinking about this.

There are 3500 species of mosquitoes,

and they're all very similar.

Some of them are so identical

that even an entomologist cannot
identify them under a microscope.

But they have an Achilles' heel.

This is what a mosquito flirting
with each other looks like.

That's a male chasing a female.

They're actually talking to each other
with their wingbeat frequencies ...

and thus, they have a signature.

We realized that using a regular phone,

a five to 10 dollar flip phone --

how many remember what this object is?

(Laughter)

We can record these acoustic
signatures from mosquitoes.

I'll tell you exactly how to do this --

I caught some mosquitoes outside.

Unlike Bill, I'm not
going to release them.

But I will tell you how
to record from this.

All you do is you tap them and they fly.

You can first test --

I can actually hear that.

And you bring your phone
which has microphones --

it turns out the mics
are so damn good already,

even on regular phones,

that you can pick up
this [near-field] signature.

And since I'm out of time,

let me just play the recording
that I made a day ago.

(Mosquitoes buzz)

This is all the charming sound
that you heard before

that you all love.

One of the contexts of this

is being able to do this
with a regular cell phone

allows us to map mosquito species.

Using a flip phone,

we mapped one of the largest
acoustic databases

with 25 to 20 species of mosquitoes
that carry human pathogens.

And from this and machine learning,

anybody who uploads this data,

we can identify and tell the probability

of what species of mosquitoes
you're actually working with.

We call this Abuzz,

and if any of you want to sign up,

just go to the website.

Let me close with something that's very
important and dear to my heart.

One of the challenges of today
is we have terrible problems.

We have a billion people
with absolutely no health care,

climate change,

biodiversity loss,

on and on and on.

And we hope that science
is going to provide the answer.

But before you leave this theatre today,

I want you to promise one thing.

We're going to make science accessible.

Not just to the people who can afford it,

but a billion others who can't.

Let's make science and scientific
literacy a human right.

The moment that you pass the tingling
feeling of making a discovery

to another child,

you're enabling them to be
the next group of people

who will actually solve these problems.

Thank you.

(Applause)