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This demonstration is how
to prepare a wet mount.
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The purpose of
doing a wet mount is
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to check for the
motility of a cell
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and also the size of a cell.
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Make sure your gloves
and your goggles are on.
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I'll be demonstrating how
to do a wet mount when
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the starting
material is a liquid
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and then when the starting
material is coming
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from a colony on a Petri plate.
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Start by taking a
slide from the box.
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You will need a cover slip.
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Cover slips are squares of
either glass or plastic,
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and they are separated by
pieces of tissue paper.
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When the starting
material is a liquid,
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take a drop of the material.
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Put it in the
center of the slide.
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Take your cover slip.
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Hold it at a 45-degree
angle, touching the slide,
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and gently drop it.
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When the starting material
is a colony on a Petri plate,
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you will take a
slide, and you're
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going to put a drop
of deionized water
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in the center of the slide.
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Then using aseptic
technique, you'll
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be using your inoculating loop.
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You'll need to sterilize
your inoculating
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loop by putting the loop into
the flame of the Bunsen burner.
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Remove the lid from the plate.
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If there's an area
on the plate where
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you can touch the loop
to cool it beforehand,
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that would be a good idea.
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Touch the surface of a colony.
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Immediately put the
lid back on the plate.
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Put the loop in
the drop of water.
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Spread it around about
the size of a dime.
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Sterilize the loop before
you put it on the bench,
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and turn off the Bunsen burner.
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Take a cover slip.
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Hold it at a 45-degree angle and
gently drop it on the sample.
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