This demonstration is how to prepare a wet mount.
The purpose of doing a wet mount is
to check for the motility of a cell
and also the size of a cell.
Make sure your gloves and your goggles are on.
I'll be demonstrating how to do a wet mount when
the starting material is a liquid
and then when the starting material is coming
from a colony on a Petri plate.
Start by taking a slide from the box.
You will need a cover slip.
Cover slips are squares of either glass or plastic,
and they are separated by pieces of tissue paper.
When the starting material is a liquid,
take a drop of the material.
Put it in the center of the slide.
Take your cover slip.
Hold it at a 45-degree angle, touching the slide,
and gently drop it.
When the starting material is a colony on a Petri plate,
you will take a slide, and you're
going to put a drop of deionized water
in the center of the slide.
Then using aseptic technique, you'll
be using your inoculating loop.
You'll need to sterilize your inoculating
loop by putting the loop into the flame of the Bunsen burner.
Remove the lid from the plate.
If there's an area on the plate where
you can touch the loop to cool it beforehand,
that would be a good idea.
Touch the surface of a colony.
Immediately put the lid back on the plate.
Put the loop in the drop of water.
Spread it around about the size of a dime.
Sterilize the loop before you put it on the bench,
and turn off the Bunsen burner.
Take a cover slip.
Hold it at a 45-degree angle and gently drop it on the sample.