WEBVTT

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This demonstration is how
to prepare a wet mount.

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The purpose of
doing a wet mount is

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to check for the
motility of a cell

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and also the size of a cell.

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Make sure your gloves
and your goggles are on.

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I'll be demonstrating how
to do a wet mount when

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the starting
material is a liquid

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and then when the starting
material is coming

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from a colony on a Petri plate.

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Start by taking a
slide from the box.

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You will need a cover slip.

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Cover slips are squares of
either glass or plastic,

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and they are separated by
pieces of tissue paper.

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When the starting
material is a liquid,

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take a drop of the material.

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Put it in the
center of the slide.

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Take your cover slip.

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Hold it at a 45-degree
angle, touching the slide,

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and gently drop it.

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When the starting material
is a colony on a Petri plate,

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you will take a
slide, and you're

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going to put a drop
of deionized water

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in the center of the slide.

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Then using aseptic
technique, you'll

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be using your inoculating loop.

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You'll need to sterilize
your inoculating

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loop by putting the loop into
the flame of the Bunsen burner.

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Remove the lid from the plate.

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If there's an area
on the plate where

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you can touch the loop
to cool it beforehand,

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that would be a good idea.

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Touch the surface of a colony.

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Immediately put the
lid back on the plate.

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Put the loop in
the drop of water.

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Spread it around about
the size of a dime.

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Sterilize the loop before
you put it on the bench,

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and turn off the Bunsen burner.

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Take a cover slip.

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Hold it at a 45-degree angle and
gently drop it on the sample.

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