[Script Info]
Title: 
[Events]
Format: Layer, Start, End, Style, Name, MarginL, MarginR, MarginV, Effect, Text
Dialogue: 0,0:00:00.00,0:00:03.78,Default,,0000,0000,0000,,This demonstration is how\Nto prepare a wet mount.
Dialogue: 0,0:00:03.78,0:00:05.81,Default,,0000,0000,0000,,The purpose of\Ndoing a wet mount is
Dialogue: 0,0:00:05.81,0:00:08.06,Default,,0000,0000,0000,,to check for the\Nmotility of a cell
Dialogue: 0,0:00:08.06,0:00:11.22,Default,,0000,0000,0000,,and also the size of a cell.
Dialogue: 0,0:00:11.22,0:00:15.17,Default,,0000,0000,0000,,Make sure your gloves\Nand your goggles are on.
Dialogue: 0,0:00:15.17,0:00:17.42,Default,,0000,0000,0000,,I'll be demonstrating how\Nto do a wet mount when
Dialogue: 0,0:00:17.42,0:00:19.52,Default,,0000,0000,0000,,the starting\Nmaterial is a liquid
Dialogue: 0,0:00:19.52,0:00:21.86,Default,,0000,0000,0000,,and then when the starting\Nmaterial is coming
Dialogue: 0,0:00:21.86,0:00:24.62,Default,,0000,0000,0000,,from a colony on a Petri plate.
Dialogue: 0,0:00:24.62,0:00:28.34,Default,,0000,0000,0000,,Start by taking a\Nslide from the box.
Dialogue: 0,0:00:28.34,0:00:30.48,Default,,0000,0000,0000,,You will need a cover slip.
Dialogue: 0,0:00:30.48,0:00:34.23,Default,,0000,0000,0000,,Cover slips are squares of\Neither glass or plastic,
Dialogue: 0,0:00:34.23,0:00:37.58,Default,,0000,0000,0000,,and they are separated by\Npieces of tissue paper.
Dialogue: 0,0:00:37.58,0:00:40.26,Default,,0000,0000,0000,,When the starting\Nmaterial is a liquid,
Dialogue: 0,0:00:40.26,0:00:42.06,Default,,0000,0000,0000,,take a drop of the material.
Dialogue: 0,0:00:42.06,0:00:45.57,Default,,0000,0000,0000,,Put it in the\Ncenter of the slide.
Dialogue: 0,0:00:45.57,0:00:48.87,Default,,0000,0000,0000,,Take your cover slip.
Dialogue: 0,0:00:48.87,0:00:52.41,Default,,0000,0000,0000,,Hold it at a 45-degree\Nangle, touching the slide,
Dialogue: 0,0:00:52.41,0:00:55.29,Default,,0000,0000,0000,,and gently drop it.
Dialogue: 0,0:00:55.29,0:00:59.22,Default,,0000,0000,0000,,When the starting material\Nis a colony on a Petri plate,
Dialogue: 0,0:00:59.22,0:01:03.24,Default,,0000,0000,0000,,you will take a\Nslide, and you're
Dialogue: 0,0:01:03.24,0:01:06.03,Default,,0000,0000,0000,,going to put a drop\Nof deionized water
Dialogue: 0,0:01:06.03,0:01:08.21,Default,,0000,0000,0000,,in the center of the slide.
Dialogue: 0,0:01:08.21,0:01:11.47,Default,,0000,0000,0000,,
Dialogue: 0,0:01:11.47,0:01:14.02,Default,,0000,0000,0000,,Then using aseptic\Ntechnique, you'll
Dialogue: 0,0:01:14.02,0:01:16.37,Default,,0000,0000,0000,,be using your inoculating loop.
Dialogue: 0,0:01:16.37,0:01:18.88,Default,,0000,0000,0000,,You'll need to sterilize\Nyour inoculating
Dialogue: 0,0:01:18.88,0:01:22.50,Default,,0000,0000,0000,,loop by putting the loop into\Nthe flame of the Bunsen burner.
Dialogue: 0,0:01:22.50,0:01:27.49,Default,,0000,0000,0000,,
Dialogue: 0,0:01:27.49,0:01:30.01,Default,,0000,0000,0000,,Remove the lid from the plate.
Dialogue: 0,0:01:30.01,0:01:31.96,Default,,0000,0000,0000,,If there's an area\Non the plate where
Dialogue: 0,0:01:31.96,0:01:35.24,Default,,0000,0000,0000,,you can touch the loop\Nto cool it beforehand,
Dialogue: 0,0:01:35.24,0:01:37.39,Default,,0000,0000,0000,,that would be a good idea.
Dialogue: 0,0:01:37.39,0:01:40.63,Default,,0000,0000,0000,,Touch the surface of a colony.
Dialogue: 0,0:01:40.63,0:01:43.81,Default,,0000,0000,0000,,Immediately put the\Nlid back on the plate.
Dialogue: 0,0:01:43.81,0:01:47.59,Default,,0000,0000,0000,,Put the loop in\Nthe drop of water.
Dialogue: 0,0:01:47.59,0:01:51.61,Default,,0000,0000,0000,,Spread it around about\Nthe size of a dime.
Dialogue: 0,0:01:51.61,0:01:57.04,Default,,0000,0000,0000,,Sterilize the loop before\Nyou put it on the bench,
Dialogue: 0,0:01:57.04,0:02:00.92,Default,,0000,0000,0000,,and turn off the Bunsen burner.
Dialogue: 0,0:02:00.92,0:02:03.02,Default,,0000,0000,0000,,Take a cover slip.
Dialogue: 0,0:02:03.02,0:02:08.83,Default,,0000,0000,0000,,Hold it at a 45-degree angle and\Ngently drop it on the sample.
Dialogue: 0,0:02:08.83,0:02:12.00,Default,,0000,0000,0000,,