[Script Info]
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Format: Layer, Start, End, Style, Name, MarginL, MarginR, MarginV, Effect, Text
Dialogue: 0,0:00:06.31,0:00:11.32,Default,,0000,0000,0000,,A saline agglutination test is a very easy in-house diagnostic procedure
Dialogue: 0,0:00:11.67,0:00:16.52,Default,,0000,0000,0000,,and should be performed in any patient with suspected hemolytic anemia.
Dialogue: 0,0:00:16.72,0:00:22.76,Default,,0000,0000,0000,,It is redundant if gross agglutination is noted in the CBC sample or the hematocrit tube.
Dialogue: 0,0:00:23.37,0:00:27.81,Default,,0000,0000,0000,,Simply place a drop of EDTA blood on to a microscope slide.
Dialogue: 0,0:00:29.76,0:00:36.04,Default,,0000,0000,0000,,Add a drop of 0.9% NaCl and mix it with the blood.
Dialogue: 0,0:00:36.14,0:00:39.46,Default,,0000,0000,0000,,Look under the microscope at the red blood cells.
Dialogue: 0,0:00:39.55,0:00:44.60,Default,,0000,0000,0000,,Check for agglutination, which is characterized by persistent clumping of cells.
Dialogue: 0,0:00:44.83,0:00:48.81,Default,,0000,0000,0000,,If you disturb the slide, the cells will stay attached to each other.
Dialogue: 0,0:00:49.22,0:00:57.61,Default,,0000,0000,0000,,It can be difficult to differentiate rouleaux formation from true agglutination because these processes can look similar at first glance.
Dialogue: 0,0:00:57.92,0:01:04.09,Default,,0000,0000,0000,,However, cells in rouleaux tend to stack rather than clump, and they dissipate when disturbed.
Dialogue: 0,0:01:05.14,0:01:12.09,Default,,0000,0000,0000,,Remember that a positive saline agglutination test indicates that there are antibodies on the surface of the erythrocytes.
Dialogue: 0,0:01:12.56,0:01:18.54,Default,,0000,0000,0000,,This supports a diagnosis of immune-mediated hemolytic anemia (IMHA).
Dialogue: 0,0:01:18.88,0:01:28.22,Default,,0000,0000,0000,,However, positive agglutination tests can occur in patients with other causes of hemolysis, such as zinc ingestion or erythrocyte parasitism.
Dialogue: 0,0:01:28.75,0:01:38.42,Default,,0000,0000,0000,,Conversely, some dogs with IMHA do not have a positive saline agglutination test, so a negative result does not exclude this diagnosis.