WEBVTT 00:00:00.000 --> 00:00:19.579 36c3 preroll music 00:00:19.579 --> 00:00:23.100 Herald: So who is excited about photography or videography? 00:00:23.100 --> 00:00:28.320 applaus Herald: Yeah? The title of the talk kind 00:00:28.320 --> 00:00:35.790 of gives us gives it away. OK. We bet we are waiting for the last people to come in 00:00:35.790 --> 00:00:44.160 and take a seat. Last time, raise your hands if you have a free seat next to you. 00:00:44.160 --> 00:00:55.289 Every one of you coming in, look for raised hands and take your seat and then we will start. 00:00:55.289 --> 00:01:19.390 Yeah, very good. OK. Looks like the doors are finally closed. Okay, so the next talk 00:01:19.390 --> 00:01:26.899 on the second day is about ultrafast imaging. So many of you have done 00:01:26.899 --> 00:01:34.149 videography or photography. Have thought about exposure time, how fast you can do 00:01:34.149 --> 00:01:39.869 your photography. And some of your might have played with lasers and have built 00:01:39.869 --> 00:01:45.540 blinky stuff with it or have done scientific experiments and Caroline Will 00:01:45.540 --> 00:01:51.140 now show us what happens if we take those to combine them and take it to the 00:01:51.140 --> 00:01:58.849 extreme. Caroline is working at DESY since four years. She has not done her PhD and 00:01:58.849 --> 00:02:05.619 is now working in a group for theoretical fast modeling of inner workings of 00:02:05.619 --> 00:02:11.280 molecules and atoms. She is doing a computational work and working together 00:02:11.280 --> 00:02:16.709 with experimentalists to verify their observations, and now she is presenting 00:02:16.709 --> 00:02:22.310 the inner mechanics of what she is doing and how we can actually maybe photograph 00:02:22.310 --> 00:02:27.720 molecules by their forming. Applause! 00:02:27.720 --> 00:02:31.160 applause 00:02:31.160 --> 00:02:35.189 Caroline: Great. Yeah. Thank you very much for the introduction and thank you very 00:02:35.189 --> 00:02:40.170 much for having me here. I'm excited to see this room so full. So I'm going to 00:02:40.170 --> 00:02:44.680 speak today about an ultrashort history of ultrafast imaging. It's a really broad 00:02:44.680 --> 00:02:48.469 topic. And I'm just gonna present some highlights, some background. Before I 00:02:48.469 --> 00:02:53.860 start, I'd like to give you a few more few more words about myself. As we've already 00:02:53.860 --> 00:02:57.620 heard, I work at DESY, this is the DESY campus you see here and in the Center for 00:02:57.620 --> 00:03:03.280 Free Electron Laser Science, circle in orange. That's where I did my PhD. So this 00:03:03.280 --> 00:03:08.530 whole campus is located in Hamburg. This is probably also a familiar place to many 00:03:08.530 --> 00:03:15.260 of you. And now this year we are in Leipzig a bit further away for the 36th 00:03:15.260 --> 00:03:21.299 Congress. So I'd like to start with a very broad question. What is the goal of 00:03:21.299 --> 00:03:25.519 ultrafast imaging? And we've heard already that ultrafast imaging is related to 00:03:25.519 --> 00:03:31.550 photography. Now, as many of you know, when you take a picture, with a quite long 00:03:31.550 --> 00:03:36.389 exposure time, you see just a blurry image, for example, in this picture of a 00:03:36.389 --> 00:03:42.510 bowl of water. We can hardly see anything. It looks a bit foggy. But if we choose the 00:03:42.510 --> 00:03:47.019 correct exposure time, which in this case is 100 times shorter in the right picture 00:03:47.019 --> 00:03:51.250 than in the left picture, then we see a clear image and we can see dynamics 00:03:51.250 --> 00:03:55.959 unfold. So we have here, a drop of water that is bouncing back from the bowl and 00:03:55.959 --> 00:04:00.470 also some ripples that are forming on the surface of this bowl. This is only visible 00:04:00.470 --> 00:04:06.329 because we chose the right exposure time. And this is to me really the key of being 00:04:06.329 --> 00:04:13.230 successful in ultrafast imaging to take a clear picture of an object that is moving. 00:04:13.230 --> 00:04:17.130 But it's not enough to say take just a picture. So now imagine you're a sports 00:04:17.130 --> 00:04:20.680 reporter. You get these two pictures and you're supposed to write up what happened. 00:04:20.680 --> 00:04:25.660 So it's complicated. So the top picture is the start, the bottom pictures is the end. 00:04:25.660 --> 00:04:30.500 Just from these two pictures, it's hard to see. But if we see before picture, we can 00:04:30.500 --> 00:04:35.169 see very complex dynamics unfold. There are particles accelerating at high 00:04:35.169 --> 00:04:41.090 velocity laughing coming in from the back. And even particles we did not see in the first 00:04:41.090 --> 00:04:46.330 picture at all somehow are very relevant to our motion. And not only skiing races 00:04:46.330 --> 00:04:52.270 are very dynamic, but most processes in nature are also not static. This is true 00:04:52.270 --> 00:04:55.889 for everything we see around ourselves, but it's especially true for everything 00:04:55.889 --> 00:05:01.370 that is quite small in the microcosm. And in general, we can gain a lot more insight 00:05:01.370 --> 00:05:08.360 from time resolved images. So from ultra short movies. I'd like to show you the 00:05:08.360 --> 00:05:12.270 very first ultrafast movie that was ever taken. Or maybe even the first movie that 00:05:12.270 --> 00:05:19.960 was taken at all. This guy, Eadweard Muybridge lived in the 19th century. And 00:05:19.960 --> 00:05:24.770 very shortly after the invention of a photography method, he tried to answer the 00:05:24.770 --> 00:05:29.900 question does a galloping horse ever lift all of its feet off the ground? Why, it's 00:05:29.900 --> 00:05:34.460 running. To us, it may seem like not so important question, but in the 19th 00:05:34.460 --> 00:05:39.560 century, the horse was the main method of transportation, and horse races were very 00:05:39.560 --> 00:05:45.490 popular. So there was a lot of interest in studying the dynamics of a horse, and this 00:05:45.490 --> 00:05:50.259 process is too fast to see with the naked eye. But Muybridge implemented a stop 00:05:50.259 --> 00:05:55.040 motion technique where the horse as it is running, cuts some wires, that then 00:05:55.040 --> 00:06:00.129 trigger photographs. And with this he was able to take these twelve photographs of 00:06:00.129 --> 00:06:05.220 the horse in motion. That was published under this title in Stanford in the 19th 00:06:05.220 --> 00:06:10.080 century. And we see very clearly in the top row third picture and maybe also 00:06:10.080 --> 00:06:14.330 second picture that indeed the horse lifts all of its legs off the ground, which was 00:06:14.330 --> 00:06:20.170 a new insight at that time. And when we stitch all of these snapshots together, we 00:06:20.170 --> 00:06:24.860 have an ultra fast movie of a horse galloping, which might be seen as the 00:06:24.860 --> 00:06:31.259 first movie that was ever made in the history of mankind. Now, when I say 00:06:31.259 --> 00:06:35.550 ultrafast today, I'm no longer thinking about horses, but about smaller things and 00:06:35.550 --> 00:06:40.570 faster things. But let's go there, very gently. So the time scale that we are all 00:06:40.570 --> 00:06:44.909 familiar with that we can see with the naked eye is something of the order of 00:06:44.909 --> 00:06:50.150 seconds. So, for example, the acceleration of this cheetah, we can see with the naked 00:06:50.150 --> 00:06:56.349 eye. Now, if we zoom in on this motion, we see that there are muscles inside of the 00:06:56.349 --> 00:07:00.930 animal that are contracting as it is running. And this muscle contraction takes 00:07:00.930 --> 00:07:06.810 place within milliseconds. So that's a part of a thousand in one second. But we 00:07:06.810 --> 00:07:11.319 can go even smaller than that to the microsecond. So proteins inside of the 00:07:11.319 --> 00:07:17.180 muscles or in any biologic matter fold and unfold on a timescale of microseconds. 00:07:17.180 --> 00:07:22.509 That's already a part in a million of a second. Now going even smaller, to 00:07:22.509 --> 00:07:29.080 nanoseconds there's certain dynamics that take place within these proteins, for 00:07:29.080 --> 00:07:34.091 example, of how they dissolve in water. But the timescale that I'm interested in 00:07:34.091 --> 00:07:38.969 today is the femtosecond. It's even faster than that it's the timescale where 00:07:38.969 --> 00:07:44.539 individual atoms move in molecules as shown in this animation. Now a 00:07:44.539 --> 00:07:49.199 femtosecond is very short. It's a part in a million of a billion of a second, or as 00:07:49.199 --> 00:07:55.039 we physicists like to call it, ten to the minus 15 seconds because it's easier to 00:07:55.039 --> 00:08:01.689 spell laughing to us. We can - to us - , we can go even faster than that. The time scale of 00:08:01.689 --> 00:08:05.389 electronic motion and in molecules would be an attosecond. I'm just mentioning it 00:08:05.389 --> 00:08:12.340 here because we don't stop at molecules, but nature is even faster than that. But 00:08:12.340 --> 00:08:15.030 for the purpose of this talk, I will mainly focus on processes that take place 00:08:15.030 --> 00:08:21.741 within the femtosecond. So within ten to the minus fifteen seconds. Now, this time 00:08:21.741 --> 00:08:26.410 scale is something that is not really related to what we think about in everyday 00:08:26.410 --> 00:08:31.150 life. But there are certain processes in chemistry, biology and physics that are 00:08:31.150 --> 00:08:36.419 really fundamental and that start at this time scale. Just to give you an idea how 00:08:36.419 --> 00:08:42.070 short a femtosecond is, the width of a human hair is about 100 micrometer. It's 00:08:42.070 --> 00:08:47.130 shown here in an electron microscopic picture. And for light at the speed of 00:08:47.130 --> 00:08:53.750 light, it takes only thirty femtoseconds to cross the hair. So that's how fast a 00:08:53.750 --> 00:08:58.950 femtosecond is. And even although this timescale is so short, there are many 00:08:58.950 --> 00:09:03.730 important processes that start here, I'd like to mention, just two of them. The 00:09:03.730 --> 00:09:07.630 first one is vision in our eyes and our retina there sits a molecule called 00:09:07.630 --> 00:09:13.660 rhodopsin, that is shown here to the left. And when light hits rhodopsin, it starts 00:09:13.660 --> 00:09:18.870 to isomorphise, which is a fancy word for saying it changes its shape. And this 00:09:18.870 --> 00:09:24.760 transmits, in the end, electrical impulses to our brain, which enables us to see. And 00:09:24.760 --> 00:09:28.850 this very first step of vision takes only two hundred femtoseconds to complete. But 00:09:28.850 --> 00:09:33.060 without it, vision would not be possible. Another very fast process that is 00:09:33.060 --> 00:09:39.649 fundamental in nature is photosynthesis, where plants take light and CO2 and 00:09:39.649 --> 00:09:45.740 convert it to other things, among them oxygen. And the very first excitation 00:09:45.740 --> 00:09:53.110 where light hits the plant and it starts to make all this energy available. That 00:09:53.110 --> 00:09:56.690 also takes less than one hundredth femtoseconds to complete. So really the 00:09:56.690 --> 00:10:01.930 fundamental questions of life lie at this timescale. And I'd like to just mention 00:10:01.930 --> 00:10:06.820 that all of these processes are not only very fast, but they also take place in 00:10:06.820 --> 00:10:11.540 very small objects, that are of a size of a few atoms to nanometers, which makes it 00:10:11.540 --> 00:10:15.480 also hard to observe because we cannot see them with the naked eye or with standard 00:10:15.480 --> 00:10:22.240 microscopes. Now, we've seen already that it's important to choose the right 00:10:22.240 --> 00:10:28.190 exposure time to get a clear image of something that's moving, but the kind of 00:10:28.190 --> 00:10:32.510 method that we need for taking such a photograph of something that is moving 00:10:32.510 --> 00:10:37.220 depends a lot on the timescale. So for stuff that is moving within seconds or 00:10:37.220 --> 00:10:43.459 fractions of a second, we can see that with the naked eye, we can use cameras to 00:10:43.459 --> 00:10:48.180 resolve faster motion, very much like Muybridge did with the very first camera. 00:10:48.180 --> 00:10:53.500 Today, of course, we can go much faster to maybe a few microseconds. With very fancy 00:10:53.500 --> 00:10:57.230 cameras called opto-electronic street cameras - i won't go into detail here - we 00:10:57.230 --> 00:11:01.560 can go down to picoseconds. So we are already very close to the motion of 00:11:01.560 --> 00:11:06.899 molecules, but we are not quite there yet. The timescale that we want to investigate 00:11:06.899 --> 00:11:11.060 is a femtosecond. So really a time timescale of molecular motion and 00:11:11.060 --> 00:11:16.550 electronics are not fast enough to reach this timescale. So we need something new. 00:11:16.550 --> 00:11:21.380 And fortunately, we can create light pulses that serve as to say flashes, but 00:11:21.380 --> 00:11:26.199 take snapshots of our moving molecules with femtosecond time resolution and light 00:11:26.199 --> 00:11:31.970 pulses can be made so short. So in the following, I'm going to show you a bit 00:11:31.970 --> 00:11:37.550 more detail on how we can use these ultra short light pulses to take snapshots of 00:11:37.550 --> 00:11:43.699 moving molecules. The first method that I would like to briefly show you is X-Ray 00:11:43.699 --> 00:11:49.120 diffraction, where we have an ultra short pulse, an X-Ray pulse coming in. It hits a 00:11:49.120 --> 00:11:54.899 sample shown here in the red bubbles. That's essentially a molecule that that we 00:11:54.899 --> 00:12:01.541 just place in the beam and it produces a so-called diffraction pattern that we can 00:12:01.541 --> 00:12:07.829 then record on a screen. Now, the whole process is quite complicated. So I like to 00:12:07.829 --> 00:12:15.420 just sketch the very basics of it. We see here X-Ray radiation hitting a crystalline 00:12:15.420 --> 00:12:23.040 sample here to the left and the sample is excited, starts to radiate X-Ray back and 00:12:23.040 --> 00:12:29.199 on the right we can see the X-Rays leaving the sample again. They will interfere and 00:12:29.199 --> 00:12:34.670 we can record this pattern on the screen. So this is what we see here in this 00:12:34.670 --> 00:12:42.570 visualization to the right. With this, we can feed a reconstructionalgorithm that 00:12:42.570 --> 00:12:47.110 allows us to transform back our diffraction pattern that we've seen here 00:12:47.110 --> 00:12:53.570 for for in this case a bio molecule. We can reconstruct from that the image as it 00:12:53.570 --> 00:13:00.899 was in real space. So this is some protein, I believe. X-ray diffraction is 00:13:00.899 --> 00:13:09.149 very nice for resolving small structures with atomic detail. Another method how we 00:13:09.149 --> 00:13:15.560 can take snapshots using ultra short pulses, that I would like to briefly 00:13:15.560 --> 00:13:20.830 introduce is absorption spectroscopy. Now you may know that light contains several 00:13:20.830 --> 00:13:27.540 colors. For example, you've surely have held a prism in hand, and the prism can 00:13:27.540 --> 00:13:32.130 break white light up into all the colors of a rainbow, that we can see with the 00:13:32.130 --> 00:13:38.149 eye. Now we can do the same with X-Ray pulses. Then we cannot see the colors 00:13:38.149 --> 00:13:44.410 anymore. So just let's just stick with a prism here. When we place a molecule in 00:13:44.410 --> 00:13:49.200 front of all these colors, the molecule will block certain colors. That's quantum 00:13:49.200 --> 00:13:56.920 mechanics. You just have to believe it or learn about it in long studies. So the 00:13:56.920 --> 00:14:03.110 molecule is placed in front of all these colors. And to be right, the absorption 00:14:03.110 --> 00:14:08.110 spectrum is recorded and the parts of the spectrum that are very bright correspond 00:14:08.110 --> 00:14:14.480 to the colors that have been blocked by the molecule. And this is a very nice 00:14:14.480 --> 00:14:19.089 technique to investigate ultra short dynamics, because where these lines are 00:14:19.089 --> 00:14:24.880 located is characteristic of the chemical elements that we find in the molecule. For 00:14:24.880 --> 00:14:28.760 example, if we use X-Ray radiation for this specific molecule, that I've shown 00:14:28.760 --> 00:14:33.810 here lysine, that's not so important which molecule it is. We have three different 00:14:33.810 --> 00:14:39.269 atoms in this molecule that are important carbon, nitrogen and oxygen and they 00:14:39.269 --> 00:14:43.880 absorb at very different colors so we can keep them apart when we take the spectrum. 00:14:43.880 --> 00:14:49.120 But not only that, we can take the spectrum at a later time when the molecule 00:14:49.120 --> 00:14:54.420 has moved around a bit and we will see that the colors, the position of the lines 00:14:54.420 --> 00:14:59.829 have changed a tiny bit. So it's really not much and I accelerated it already in 00:14:59.829 --> 00:15:06.070 this visualization quite a bit. But with experimental methods, we can resolve this. 00:15:06.070 --> 00:15:11.420 And this allows us to then trace back to how the molecule was moving in between 00:15:11.420 --> 00:15:16.889 when we took these two snapshots. There are many more methods that you can use to 00:15:16.889 --> 00:15:21.820 take ultrafast images. So we call them probe signals because we probe the 00:15:21.820 --> 00:15:26.889 ultrafast motion of a molecule with such an ultra short pulse. For example, we can 00:15:26.889 --> 00:15:33.009 record photo electrons or we can record fragments of a molecule and many more. But 00:15:33.009 --> 00:15:37.519 I won't go into further detail here because this is not an exhaustive list of 00:15:37.519 --> 00:15:42.320 methods that we can use. I'd rather like to show you how we can take molecular 00:15:42.320 --> 00:15:47.709 movies so how we can combine all these ultrashort pulses to in the end film a 00:15:47.709 --> 00:15:55.050 molecule in action. Now we've already seen in the movie of the horse that we need to 00:15:55.050 --> 00:16:00.990 stitch several snapshots together and then we have a full picture, full motion of a 00:16:00.990 --> 00:16:06.740 molecule. So we just like to do the same, but ten to the 15 times faster, should not 00:16:06.740 --> 00:16:13.029 be too difficult, right? So we use our ultra short pulse. First ultrasound parts 00:16:13.029 --> 00:16:17.980 that we use as a trigger, parts that sets off the motion and the molecule. This 00:16:17.980 --> 00:16:22.410 defines us a certain time zero in our experiment and makes it sort of repeatable 00:16:22.410 --> 00:16:28.300 because we always start the same kind of motion by giving it a small hit and now 00:16:28.300 --> 00:16:34.870 it's just moving around. So we wait for a certain time, a time delay and then come 00:16:34.870 --> 00:16:40.940 in with a probe pulse. The probe pulse takes a snapshot of a molecule. This goes 00:16:40.940 --> 00:16:45.320 to some detector, goes to a kind of complicated reconstruction method that we 00:16:45.320 --> 00:16:51.709 just execute from our screen. And with this, we reconstruct a snapshot of a 00:16:51.709 --> 00:16:56.850 molecule. But this is only one snapshot and we want a whole movie. So we need to 00:16:56.850 --> 00:17:02.240 repeat this process over and over again by shining and more and more probe pulses. 00:17:02.240 --> 00:17:08.040 And this will create more and more snapshots of a molecule. And in the end, 00:17:08.040 --> 00:17:12.209 we could stitch all of these together and we would arrive at the same image that you 00:17:12.209 --> 00:17:18.430 see in the in the middle where the molecules is happily moving around. There 00:17:18.430 --> 00:17:24.790 is one little problem: The probe pulse typically destroys the molecule. This is 00:17:24.790 --> 00:17:28.500 very different. This is very different from taking pictures of a horse. The horse 00:17:28.500 --> 00:17:36.289 normally survives. laughting So the probe pulse destroys the molecule. It just goes away. 00:17:36.289 --> 00:17:41.830 So for each of these snapshots we need to use a new molecule. So we typically have a 00:17:41.830 --> 00:17:47.970 stream of samples that is falling from the top to the bottom in our experiment. And 00:17:47.970 --> 00:17:51.690 then we have to carefully align two pulses a trigger pulse and a probe pulse that 00:17:51.690 --> 00:17:57.610 come together and take a snapshot of this molecule. And of course, we have to find a 00:17:57.610 --> 00:18:03.120 method on how to make identical molecules available in - Yeah - you see, there's a 00:18:03.120 --> 00:18:07.860 lot of complications with doing these experiments that I'm completely leaving 00:18:07.860 --> 00:18:15.090 out here. So now we want to take a molecular movie and we know that we want 00:18:15.090 --> 00:18:19.700 to have ultra short pulses to do so. But I didn't tell you yet what kind of light 00:18:19.700 --> 00:18:24.750 source we need. So there are many light sources all around us. We have here lights 00:18:24.750 --> 00:18:29.450 from lamps. I have a light in my laser pointer with light from the sun. But we 00:18:29.450 --> 00:18:35.380 need quite specific light sources to take these snapshots of molecular motion. We've 00:18:35.380 --> 00:18:37.970 already established that we want ultrashort pulses because else we cannot 00:18:37.970 --> 00:18:44.260 resolve femtosecond dynamics, but for the proper kind of wavelength that we need I 00:18:44.260 --> 00:18:47.980 would like to quickly remind you of the electromagnetic spectrum that you've 00:18:47.980 --> 00:18:54.090 probably seen at some point in high school. So, so light, as you see here in 00:18:54.090 --> 00:18:58.970 the bottom picture is an electromagnetic wave that comes in different wavelengths. 00:18:58.970 --> 00:19:05.440 They can be quite long as in the case of radio waves to the very left. Then we have 00:19:05.440 --> 00:19:08.470 the region of visible light shown here as the rainbow that we can perceive with our 00:19:08.470 --> 00:19:14.270 eyes. And then we have wavelengths that are too short to see with our eyes. First, 00:19:14.270 --> 00:19:18.880 UV radiation, that gives us a tan in the summer if we leave our house and then we 00:19:18.880 --> 00:19:25.400 have X-ray radiation, soft and hard X-ray radiation that have atomic wavelength. So 00:19:25.400 --> 00:19:30.570 the wavelength is really on the order of the size of an atom. So what kind of 00:19:30.570 --> 00:19:37.080 wavelength do we need to study ultra short dynamics - ultra fast dynamics? We can 00:19:37.080 --> 00:19:43.321 first think about what kind of wavelength we need when we want to construct an ultra 00:19:43.321 --> 00:19:49.600 short pulse. I've drawn here two pulses to the left, a slightly longer pulse to the 00:19:49.600 --> 00:19:53.980 right, a shorter pulse. And now if you think about squeezing the left parts 00:19:53.980 --> 00:19:58.610 together such that it becomes shorter and shorter, you see visually that the 00:19:58.610 --> 00:20:03.890 wavelength also needs to shrink. So we need shorter wavelengths for the shorter 00:20:03.890 --> 00:20:10.350 the pulse we want to make. So this will be located somewhere here in this region of 00:20:10.350 --> 00:20:15.450 the electromagnetic spectrum. And another important thing that we need to keep in 00:20:15.450 --> 00:20:21.250 mind is if we want to take pictures by X-ray diffraction, we are limited, so we 00:20:21.250 --> 00:20:27.429 can only resolve structures that are about the same size as the wavelength we used to 00:20:27.429 --> 00:20:31.919 take our diffraction image. So if we want to take a picture of something with atomic 00:20:31.919 --> 00:20:37.549 resolution, our wavelength needs to be of atomic size as well. And this places us in 00:20:37.549 --> 00:20:47.169 the region of X-Rays drawn here, that have a wavelength of less than a nanometer. So 00:20:47.169 --> 00:20:51.510 we can establish that we want small wavelengths in general. We have two 00:20:51.510 --> 00:20:55.840 additional requirements that would just touch upon very briefly. First, we need 00:20:55.840 --> 00:21:01.460 very brilliant pulses because the pulses are so short, we need to have a lot of 00:21:01.460 --> 00:21:06.760 light in the short pulse. You can think about taking a picture in a dark room with 00:21:06.760 --> 00:21:12.270 a bad camera. You won't see anything. So we need very bright flashes of light. 00:21:12.270 --> 00:21:16.159 Another requirement is we need coherent laser light. So we cannot just use any 00:21:16.159 --> 00:21:20.130 light, but it needs to have certain properties like laser light. 00:21:20.130 --> 00:21:25.110 Unfortunately, the lasers that you can buy commercially do not operate in the region 00:21:25.110 --> 00:21:29.110 of the electromagnetic spectrum that we are interested in. So we need to come up 00:21:29.110 --> 00:21:34.830 with something new. And I will show you how we can generate ultra short pulses 00:21:34.830 --> 00:21:39.380 both in the laboratory where we can generate pulses that are very short and 00:21:39.380 --> 00:21:46.650 extend up to maybe the soft X-ray region. And another method to generate ultra short 00:21:46.650 --> 00:21:53.380 pulses is at free electron laser sources, where we can go really to the hard X-Ray 00:21:53.380 --> 00:21:59.480 regime. But first I'd like to go to the laboratory. So in the laboratory, it's 00:21:59.480 --> 00:22:03.380 possible to generate an ultrashort pulse by using a process that's called high 00:22:03.380 --> 00:22:08.201 harmonic generation. In high harmonic generation we start off of a high 00:22:08.201 --> 00:22:13.470 intensity pulse, that's a red pulse coming in from the left, which which is focused 00:22:13.470 --> 00:22:19.350 in a gas cell. And from there, it generates new frequencies of light. So the 00:22:19.350 --> 00:22:24.770 light that comes out is no longer red, but it's violet, blue. We cannot see it with 00:22:24.770 --> 00:22:27.980 the naked eye. So that's an artist's impression of how high harmonic generation 00:22:27.980 --> 00:22:33.470 works. Before going into more detail about why this method is so good at producing 00:22:33.470 --> 00:22:38.770 ultra short pulses, I'd like to mention that this is only possible because we have 00:22:38.770 --> 00:22:44.120 the high intensity driving pulses, the red laser pulses available. This goes back to 00:22:44.120 --> 00:22:47.270 work by Donna Strickland and Gerard Mourou, who were awarded the Nobel Prize 00:22:47.270 --> 00:22:54.950 in the year 2018 in physics for this work that has been done in the 80s. Now we're 00:22:54.950 --> 00:22:59.690 coming to the only equation of his talk, which is this equation that relates the 00:22:59.690 --> 00:23:07.500 energy width and the time duration of a ultra short pulse. By the law of fourier 00:23:07.500 --> 00:23:13.150 limits we cannot have pulses that are very short in time and at the same time very 00:23:13.150 --> 00:23:17.700 narrow in energy. But we need to choose one. So if we want to have policies that 00:23:17.700 --> 00:23:23.640 are very short in time like the pulse that I've shown here on the bottom, that is 00:23:23.640 --> 00:23:26.669 actually only two hundred fifty attoseconds long, so even shorter than a 00:23:26.669 --> 00:23:33.059 femtosecond, then we need to have a very broad width in energy. And this means 00:23:33.059 --> 00:23:37.450 combining a lot of different colors inside of this pulse. And this is what makes high 00:23:37.450 --> 00:23:41.580 harmonic generation so efficient at creating ultra short pulses, because the 00:23:41.580 --> 00:23:47.419 spectrum that the colors that come out of high harmonic generation are shown here 00:23:47.419 --> 00:23:52.080 and they really span a long width. So we get a lot of different colors with about 00:23:52.080 --> 00:23:57.669 the same intensity. And you can think of it like putting them all back together 00:23:57.669 --> 00:24:04.990 into one attosecond pulse. That is very short in time. This method has really made 00:24:04.990 --> 00:24:08.950 a big breakthrough in the generation of ultra short laser pulses we see here a 00:24:08.950 --> 00:24:15.300 plot of a time duration of laser pulses versus the year, and we see that since the 00:24:15.300 --> 00:24:23.520 invention of the laser, here in the mid 60s, there was a first technological 00:24:23.520 --> 00:24:28.920 progress and shorter and shorter pulses could be generated. But then in the 80s, 00:24:28.920 --> 00:24:34.399 there was a limit that had been reached of about five femtoseconds, I believe. And we 00:24:34.399 --> 00:24:39.710 could not really go farther than that and only with high harmonic generation, that 00:24:39.710 --> 00:24:45.419 sets in here shortly before the year 2000, we were able to generate pulses that are 00:24:45.419 --> 00:24:51.760 of a femtosecond duration. So that really touch the timescale of molecular motion. 00:24:51.760 --> 00:24:57.200 The current world record is a pulse, that is only 43 attoseconds long, established 00:24:57.200 --> 00:25:02.289 in the year 2017. So that's really the timescale of electrons and we can do all 00:25:02.289 --> 00:25:06.649 sorts of nice experiments with it where we directly observe electronic motion in 00:25:06.649 --> 00:25:12.470 atoms and molecules. This is all very nice, but it has one limitation: We cannot 00:25:12.470 --> 00:25:17.169 go to hard X-rays, at least not right now. So high harmonic generation cannot produce 00:25:17.169 --> 00:25:23.000 the kind of very short wavelengths that we need in order to to do X-ray diffraction 00:25:23.000 --> 00:25:29.429 experiments with atomic resolution. So if we want to have ultra short pulses that 00:25:29.429 --> 00:25:35.730 have X-Ray wavelengths, we need to build right now very complex, very big machines, 00:25:35.730 --> 00:25:43.080 the so-called free electon lasers. Now, this would be a specific light source that 00:25:43.080 --> 00:25:48.059 can produce ultra short pulses with X-ray wavelengths in itself. The X-Ray 00:25:48.059 --> 00:25:52.720 wavelengths is not so new. We know how to take X-ray images for about one hundred 00:25:52.720 --> 00:25:58.910 and thirty years and already in the 50s. Rosalind Franklin, who is looking at a 00:25:58.910 --> 00:26:05.110 microscope here, was able to take a picture of DNA, an X-ray diffraction 00:26:05.110 --> 00:26:11.880 pattern of a DNA double helix that was successful in revealing the double helix 00:26:11.880 --> 00:26:19.370 structure of our genetic code. But this is not a time resolved measurement. So think 00:26:19.370 --> 00:26:24.650 of it as you have a molecule that is in crystalline form, so it's not moving 00:26:24.650 --> 00:26:32.480 around and we can just take an X-ray image of it, it's not going anywhere. But if we 00:26:32.480 --> 00:26:36.860 want - if we want to take a picture of something that is moving, we need to have 00:26:36.860 --> 00:26:43.400 very short pulses. But we still need the same number of what we call photons, light 00:26:43.400 --> 00:26:48.890 particles. Or think of it as we need more brilliant X-ray flashes of light than we 00:26:48.890 --> 00:26:55.140 could obtain before. And there was very nice technological development in the past 00:26:55.140 --> 00:27:01.690 50 years or so, where we were able to go from the X-ray tube to newer light sources 00:27:01.690 --> 00:27:07.260 called Synchrotron, and today, free electro lasers that always increase the 00:27:07.260 --> 00:27:12.180 peak brilliance in an exponential way. So we can take really brilliant, really 00:27:12.180 --> 00:27:17.270 bright X-ray flashes right now. I cannot go into the details of all of that, but I 00:27:17.270 --> 00:27:21.350 found a very nice talk from two years ago, but actually explains everything from 00:27:21.350 --> 00:27:28.590 Synchrotron to FELs still available online if you're interested in this work. And as 00:27:28.590 --> 00:27:32.110 always, if something is failing scaling exponentially, most of you will be 00:27:32.110 --> 00:27:37.840 familiar with Moore's Law, that tells us about the exponential scaling of 00:27:37.840 --> 00:27:45.169 transistors. If something grows this fast, it really opens up a new series of 00:27:45.169 --> 00:27:49.299 experiments of new technological applications that no one has thought of 00:27:49.299 --> 00:27:55.600 before. And the same is true with free electron lasers. So I'm going to focus 00:27:55.600 --> 00:28:00.450 just on the most brilliant light sources for X-rays. Right now, the free electron 00:28:00.450 --> 00:28:06.230 lasers that are at the top right here of this graph have been around for maybe 10 00:28:06.230 --> 00:28:13.220 years or so. I cannot go into a lot of detail on how to generate ultrashort 00:28:13.220 --> 00:28:18.220 pulses with X-Rays. So I'd like to give you just a very broad picture of how 00:28:18.220 --> 00:28:23.909 this works. First, we need a bunch of electrons, that is accelerated to 00:28:23.909 --> 00:28:29.640 relativistic speed. This sounds very easy, but is actually part of a two kilometer 00:28:29.640 --> 00:28:35.490 long accelerator, that we have to build and maintain. Now we have this bunch here 00:28:35.490 --> 00:28:41.230 of electrons shown in red and it's really fast and now we can bring it into 00:28:41.230 --> 00:28:46.519 something that is called an undulator. That's a series of alternating magnets, 00:28:46.519 --> 00:28:52.270 shown here in green on blue for the alternating magnets. And you may remember, 00:28:52.270 --> 00:28:57.100 that when we put an electron, that is as a charged particle, into a magnetic field, 00:28:57.100 --> 00:29:02.820 the Lorence force will drive it away. And if you have alternating magnets, then the 00:29:02.820 --> 00:29:08.890 electron will go on a sort of wiggly path in this undulator. And the electron is a 00:29:08.890 --> 00:29:13.890 charged particle as it is wiggling around wherever it turns around, it will emit 00:29:13.890 --> 00:29:18.309 radiation, that happens to be in the X-ray region of the electromagnetic spectrum, 00:29:18.309 --> 00:29:23.260 which is exactly what we want. We can watch this little movie here to see a 00:29:23.260 --> 00:29:28.800 better picture. So this is the undulating seeing from the side. We now go inside of 00:29:28.800 --> 00:29:35.880 the undulator. We have a series of alternating magnets. Now the electron 00:29:35.880 --> 00:29:42.430 bunch shows up and you see the wiggly motion as it passes the different magnets. 00:29:42.430 --> 00:29:48.390 And you see the bright X-Ray flash that is formed and gets stronger and stronger as 00:29:48.390 --> 00:29:54.779 the electron bunch passes the undulator. So we need several of these magnet pairs to 00:29:54.779 --> 00:30:00.130 in the end, get the very bright X-Ray flash. And at the end of the undulator we 00:30:00.130 --> 00:30:06.240 dump the electron, we don't really need this electron bunch anymore and continue 00:30:06.240 --> 00:30:13.830 with a very bright X-Ray flash. This whole process is a bit stochastic in nature, but 00:30:13.830 --> 00:30:18.890 it's amplifying itself in because of the undulator. This is why the longer the 00:30:18.890 --> 00:30:29.020 undulator is, the more bright X-Ray flashes we can generate. This whole thing 00:30:29.020 --> 00:30:33.149 is kind of complicated to build, it's a very complex machine. So right now there 00:30:33.149 --> 00:30:37.740 are only very few free electron lasers in the world. First one in California called 00:30:37.740 --> 00:30:43.850 LCLS 1, currently being upgraded to LCLS 2. There are several in Europe. There's 00:30:43.850 --> 00:30:49.330 one in Switzerland, in Italy and Hamburg. So there's a Flash that does not operate 00:30:49.330 --> 00:30:54.559 in the hot X-ray regime, but was kind of first free electron laser. That's the most 00:30:54.559 --> 00:30:58.919 recent addition to the free electron laser zoo. It's the European XFEL also located 00:30:58.919 --> 00:31:04.399 in Hamburg. And then we have some of these light sources in Asia, in Korea, South 00:31:04.399 --> 00:31:10.730 Korea, Japan, and one currently under construction in Shanghai. I'd like to show 00:31:10.730 --> 00:31:15.740 you a bit more details about the European X-ray free electron laser, because it's 00:31:15.740 --> 00:31:23.980 closest to us, and at least closest to where I work. So the European XFEL is a 00:31:23.980 --> 00:31:30.049 three point four kilometer long machine that is funded by in total 12 countries, 00:31:30.049 --> 00:31:36.350 So Germany and Russia paying the most and then the other 10 countries also providing 00:31:36.350 --> 00:31:41.130 to the construction and maintenance costs. This machine starts at the DESY campus, 00:31:41.130 --> 00:31:47.340 but as shown here to the right of the picture. And then we have first an 00:31:47.340 --> 00:31:51.580 accelerator line for the electrons that it's already one point seven kilometers 00:31:51.580 --> 00:31:58.450 long and where we add electrons reach their relativistic speed. Then the 00:31:58.450 --> 00:32:04.600 undulate comes in, so the range of magnets where we X-Ray flashes are produced. The x 00:32:04.600 --> 00:32:09.330 X-Ray flashes then cross the border to Schleswig-Holstein, laughter shown here, on the 00:32:09.330 --> 00:32:16.450 other side in a new federal state. They reach the experimental hall. We have in 00:32:16.450 --> 00:32:21.049 total six experimental end stations at the European XFEL that provide different 00:32:21.049 --> 00:32:24.419 instrumentation, depending on which kind of system you want to study, you need 00:32:24.419 --> 00:32:30.960 slightly different instruments. And it's not only for taking molecular movies, but 00:32:30.960 --> 00:32:36.010 the XFEL is used, among others, for material science, for the imaging of bio 00:32:36.010 --> 00:32:40.950 molecules, for femtosecond chemistry, all sorts of things. So really wide range of 00:32:40.950 --> 00:32:46.580 applications. It's right now the the fastest such light source can take twenty 00:32:46.580 --> 00:32:51.580 seven thousand flashes per second, which is great because every flash is one 00:32:51.580 --> 00:32:56.110 picture. So if we want to take a lot of snapshots, if you want to generate a lot 00:32:56.110 --> 00:33:01.179 of data in a short time, it's great to have as many flashes per second as 00:33:01.179 --> 00:33:08.720 possible. And as you can imagine, it's kind of expensive since there are so few 00:33:08.720 --> 00:33:15.450 free electron lasers in the world to take measurements there. The complete price tag 00:33:15.450 --> 00:33:19.919 for constructing this machine, it took eight years and cost one point two billion 00:33:19.919 --> 00:33:25.580 euros, which may seem a lot, but it's the same amount that we spend on concert halls 00:33:25.580 --> 00:33:39.029 in Hamburg. loud laughter applause So kind of comparable. Now, when you factor in maintenance and so on, 00:33:39.029 --> 00:33:45.639 I think a minute of X-Ray beam at such an XFEL cost several thousands of tens of 00:33:45.639 --> 00:33:51.570 thousands of euros in the end. So getting measurement time is complicated and there 00:33:51.570 --> 00:33:56.710 are committees that select the most fruitful approaches and so on. So in order 00:33:56.710 --> 00:34:04.000 to not to waste or do taxpayers money. With this, I'd like to make a small 00:34:04.000 --> 00:34:08.020 comparison of the light sources that I've introduced now. So I introduced the 00:34:08.020 --> 00:34:12.810 laboratory light sources and the XFEL light source. In general, in the 00:34:12.810 --> 00:34:17.600 laboratory we can generate very short pulses of less than 100 attoseconds by now 00:34:17.600 --> 00:34:22.980 and in the XFEL we are limited to something about 10 femtoseconds right now. 00:34:22.980 --> 00:34:29.630 In terms of brilliance the XFELs can go to much more bright pulses, simply because 00:34:29.630 --> 00:34:32.960 they are bigger machines and high harmonic generation in itself is a kind of 00:34:32.960 --> 00:34:38.870 inefficient process. In terms of wavelength X-Ray free electron lasers 00:34:38.870 --> 00:34:42.620 enable us to reach these very short wavelengths with X-Rays, that we need to 00:34:42.620 --> 00:34:48.290 get atomic resolution of defractive images. In the laboratory we are a bit 00:34:48.290 --> 00:34:54.850 more limited to maybe the soft X-ray region. There's another important thing to 00:34:54.850 --> 00:34:59.890 keep in mind when we do experiments, that's the control of pulse parameters. So 00:34:59.890 --> 00:35:03.040 is every pulse that comes out of my machine the same as the one that came out 00:35:03.040 --> 00:35:08.520 of my machine before. And since the XFEL produces pulses by what is in the end, a 00:35:08.520 --> 00:35:13.630 stochastic process, that's not really the case. So the control of possible 00:35:13.630 --> 00:35:20.400 parameters is not really given. This is much better in the laboratory. And in 00:35:20.400 --> 00:35:23.280 terms of cost and availability, it would of course, be nice if we could do more 00:35:23.280 --> 00:35:29.620 experiments in the lab. Then at the XFEL simply because we XFEL ls so expensive to 00:35:29.620 --> 00:35:35.730 build and maintain and we have so few of them in the world. And you can see this 00:35:35.730 --> 00:35:41.840 tunnel here. It stretches for two kilometers or so, all packed with very 00:35:41.840 --> 00:35:53.260 expensive equipment. So I'd like to show you a brief example of what we can learn 00:35:53.260 --> 00:35:58.500 in ultrafast science. So this is a theoretical work that we did in our group. 00:35:58.500 --> 00:36:03.720 So no experimental data, but still nice to see. This is concerned with an organic 00:36:03.720 --> 00:36:09.730 solar cell. So we all know solar cells. They convert sunlight to electric energy 00:36:09.730 --> 00:36:14.480 that we can use in our devices. The nice thing about organic solar cells is that 00:36:14.480 --> 00:36:20.900 they are foldable, very lightweight, and we can produce them cheaply. The way that 00:36:20.900 --> 00:36:25.630 such a solar cell works is we have light shining in and at the bottom of the solar 00:36:25.630 --> 00:36:29.410 cell there sits an electrode that collects all the charges and creates an electric 00:36:29.410 --> 00:36:33.570 current. Now light creates a charge that somehow needs to travel down there to this 00:36:33.570 --> 00:36:42.000 electrode and in fact, many of these charges. So the important thing where we 00:36:42.000 --> 00:36:46.640 build such an organic solar cell is that we need a way to efficiently transport 00:36:46.640 --> 00:36:55.240 these charges. And we can do so by putting polymers inside. A polymer is just a 00:36:55.240 --> 00:36:59.670 molecule that is made up of two different or two or more different smaller 00:36:59.670 --> 00:37:05.100 molecules. And one such polymer, which should be very efficient at transporting 00:37:05.100 --> 00:37:10.170 these charges is BT-1T, that is shown here of a name is not so important, it's an 00:37:10.170 --> 00:37:15.080 abbreviation. Because in BT-1T when we create a charge at one end of a molecule 00:37:15.080 --> 00:37:20.180 here at the top, it travels very quickly to the other side of a molecule and you 00:37:20.180 --> 00:37:26.880 can imagine stacking several of these BT-1T or especially of the Ts together, 00:37:26.880 --> 00:37:31.170 putting it in this material. And then we have a very efficient flow of energy in 00:37:31.170 --> 00:37:41.880 our organic solar cell. So what we did was we calculated the ultrafast charge 00:37:41.880 --> 00:37:48.161 migration in BT-1T, shown here to the right. The pink thing is the charge 00:37:48.161 --> 00:37:53.570 density that was created by an initial ionization of the molecule. And now I show 00:37:53.570 --> 00:37:58.110 you the movie, how this charge is moving around in a molecule so you can see 00:37:58.110 --> 00:38:02.820 individual atoms moving, the whole molecules vibrating a bit. And the charge, 00:38:02.820 --> 00:38:10.570 if you look closely, is locating on the right half of a molecule within about 250 00:38:10.570 --> 00:38:16.780 femtoseconds. Now, we cannot observe this charge migration directly by looking at 00:38:16.780 --> 00:38:21.290 this pink charge density that I've drawn here, because it's at least for us, not 00:38:21.290 --> 00:38:26.151 experimentally observable directly. So we need an indirect measurement, an X-Ray 00:38:26.151 --> 00:38:30.050 absorption spectroscopy that I showed you in the beginning could be such a 00:38:30.050 --> 00:38:35.310 measurement. Because in the X-Ray absorption spectrum of BT-1T that I've 00:38:35.310 --> 00:38:41.030 shown here in the bottom left, we see distinct peaks depending on where the 00:38:41.030 --> 00:38:47.070 charge is located. Initially the charge is located at the top sulfur atom here and 00:38:47.070 --> 00:38:53.780 this molecule and we will see a peek at this color. Once the charge moves away to 00:38:53.780 --> 00:38:58.060 the bottom of a molecule to the other half, we will see a peak at the place 00:38:58.060 --> 00:39:02.680 where nothing is right now because the charge is not there. But if I start this 00:39:02.680 --> 00:39:09.600 movie, we will again see very fast charge transfer. So within about two hundred 00:39:09.600 --> 00:39:14.050 femtoseconds, the charge goes from one end to the molecule to the other end of a 00:39:14.050 --> 00:39:19.470 molecule. And it would be really nice to see this in action in the future XFEL 00:39:19.470 --> 00:39:25.720 experiment. But the process is very long. You need to apply for time at an XFEL. You 00:39:25.720 --> 00:39:29.850 need to evaluate all the data. So maybe a couple of years from now we will have the 00:39:29.850 --> 00:39:37.730 data available. Right now we are stuck with this movie, that we calculated. Now, 00:39:37.730 --> 00:39:43.590 towards the end of my talk, I'd like to go beyond the molecular movie. So I've shown 00:39:43.590 --> 00:39:47.860 you now how to generate the light pulses and an example of what we can study with 00:39:47.860 --> 00:39:53.540 these light pulses. But this is not all we can do: So when you think of a chemical 00:39:53.540 --> 00:39:58.420 reaction, you might remember high school chemistry or something like this, which is 00:39:58.420 --> 00:40:03.460 always foaming and exploding and nobody really knows what is going on. So a 00:40:03.460 --> 00:40:08.480 chemical reaction quite naturally involves molecular dynamics, for example, the 00:40:08.480 --> 00:40:13.180 decomposition of a molecule to go from here, from the left side to the right 00:40:13.180 --> 00:40:18.180 side, the molecules will somehow need to rearrange so all the atoms will have moved 00:40:18.180 --> 00:40:24.340 quite a bit. We've seen already how we can trigger these chemical reactions or these 00:40:24.340 --> 00:40:29.770 molecular motion that was part of a molecular movie. But it would be really 00:40:29.770 --> 00:40:34.430 cool if we could control the reaction with light. So the way to do this, it's not 00:40:34.430 --> 00:40:38.920 currently something that is possible, but maybe in the near future, would be to 00:40:38.920 --> 00:40:44.440 implement a sort of optimisation feedback loop. So we would record the fragments of 00:40:44.440 --> 00:40:48.970 our reaction, send it to an optimization routine that will also be quite 00:40:48.970 --> 00:40:53.620 complicated and will need to take into account the whole theory of how light and 00:40:53.620 --> 00:40:58.770 matter, interact and so on. And this optimization routine would then generate a 00:40:58.770 --> 00:41:03.800 new sequence of ultra short pulses and with this feedback loop, it might be 00:41:03.800 --> 00:41:10.100 possible to find the right pulses to control chemical reactions, taking into 00:41:10.100 --> 00:41:17.640 account the quantum nature of this motion and so on. Right now this is not possible. 00:41:17.640 --> 00:41:23.651 First, because the whole process of how we can generate these ultra short pulses is 00:41:23.651 --> 00:41:28.760 not so well controlled that we could actually implement it in such a loop. And 00:41:28.760 --> 00:41:33.000 also the step optimization routine is more complex than it looks like here in this 00:41:33.000 --> 00:41:39.490 picture. So this is something that people are working on at the moment, but this 00:41:39.490 --> 00:41:44.300 would be something like the ultra fast wishlist for next Christmas, not this 00:41:44.300 --> 00:41:49.710 Christmas. So we've succeeded in taking a molecular movie, but we would also like to 00:41:49.710 --> 00:41:55.270 be able to direct a molecular movie. So to go beyond just watching nature, but 00:41:55.270 --> 00:42:01.460 controlling nature because this is what humans like to best, laughing fortunately or 00:42:01.460 --> 00:42:06.230 unfortunately, it depends. So I'd like to just show you that this is really an ultra 00:42:06.230 --> 00:42:12.220 fast developing field. There's lots of new research papers every day, every week 00:42:12.220 --> 00:42:18.070 coming in, studying all sorts of systems. When you just take a quick and dirty 00:42:18.070 --> 00:42:22.820 metric of how important ultrafast science is this is the number of articles per year 00:42:22.820 --> 00:42:28.500 that mentioned ultrafast in Google Scholar, it's exponentially growing. At 00:42:28.500 --> 00:42:30.780 the same time, the number of total publications in Google Scholar is more or 00:42:30.780 --> 00:42:34.990 less constant, so the blue line here outgrows the green line considerably since 00:42:34.990 --> 00:42:45.070 about ten years. So what remains to be done? We've seen that we have light 00:42:45.070 --> 00:42:50.020 sources available to generate ultra short pulses, but as always, when you have 00:42:50.020 --> 00:42:55.540 better machines, bigger machines, you can take more fancy experiments. So it would 00:42:55.540 --> 00:43:00.580 be really nice to develop both lab based sources and free electron laser sources so 00:43:00.580 --> 00:43:06.360 that we can take more, more interesting, more complex experiments. Another 00:43:06.360 --> 00:43:10.060 important challenge, that's what people in my research group where I work are working 00:43:10.060 --> 00:43:16.750 on is to improve theoretical calculations because I did not go into a lot of detail 00:43:16.750 --> 00:43:21.730 on how to calculate these things, but it's essentially quantum mechanics and quantum 00:43:21.730 --> 00:43:26.530 mechanics skales very unfavorably. So going from a very small molecule like the 00:43:26.530 --> 00:43:32.490 glycene molecule here to something like a protein is not doable. Simply, it cannot 00:43:32.490 --> 00:43:38.100 compute this with quantum mechanics. So we need all sorts of new methodology to - 00:43:38.100 --> 00:43:45.100 Yeah - to better describe larger systems. We would in general like to study not only 00:43:45.100 --> 00:43:49.410 small molecules and not only take movies of small molecules, but really study large 00:43:49.410 --> 00:43:55.710 systems like this is the FMO complex, that is a central in photosynthesis or solid 00:43:55.710 --> 00:44:01.410 states that are here shown in this crystal structure simply because this is more 00:44:01.410 --> 00:44:07.790 interesting for biological, chemical applications. And finally, as I've shown 00:44:07.790 --> 00:44:13.460 you, it would be cool to directly control chemical reactions with light. So to find 00:44:13.460 --> 00:44:20.810 a way how to replace this mess with a clean light pulse. With this, I'm at the 00:44:20.810 --> 00:44:26.240 end. I'd like to quickly summarize femtodynamics, really fundamental in 00:44:26.240 --> 00:44:31.500 biology, chemistry and physics. So more or less, the origin of life is on this 00:44:31.500 --> 00:44:36.840 timescale. We can take molecular movies with ultrashort laser pulses and we can 00:44:36.840 --> 00:44:41.650 generate these pulses in the laboratory or add free electron lasers with different 00:44:41.650 --> 00:44:46.820 characteristics. And we would like to not only understand these ultra fast 00:44:46.820 --> 00:44:51.920 phenomena, but we would also like to be able to control them in the future. With 00:44:51.920 --> 00:44:54.160 this, I'd like to thank you for your attention and thank the supporting 00:44:54.160 --> 00:45:04.570 institutions here that funded my PhD work. 00:45:04.570 --> 00:45:10.820 applause 00:45:10.820 --> 00:45:17.380 Herald: Well, that was an interesting talk. I enjoyed it very much. I guess this 00:45:17.380 --> 00:45:23.450 will spark some questions. If you want to ask Caroline a question, please line up 00:45:23.450 --> 00:45:28.900 behind the microphones. We have three in the isles between the seats if you want to 00:45:28.900 --> 00:45:36.070 leave, please do so in the door here in the front. And until we get questions from 00:45:36.070 --> 00:45:39.570 the audience, do we have questions from the Internet? 00:45:39.570 --> 00:45:45.311 Signal angel: Yes. Big fat random user is curious about the design of the X-Ray 00:45:45.311 --> 00:45:48.460 detector. Do you have any information on that? 00:45:48.460 --> 00:45:56.030 Caroline: That's also very complex. I'm not a big expert in detectors. At this 00:45:56.030 --> 00:45:58.460 point, I really recommend watching the talk from two years ago, that explains a 00:45:58.460 --> 00:46:05.140 lot more about the X-Ray detectors. So what I know about the X-Ray detector is 00:46:05.140 --> 00:46:09.990 that it's very complicated to process all the data because when you have 27000 00:46:09.990 --> 00:46:16.370 flashes of light, it produces, I think terabytes of data within seconds and you 00:46:16.370 --> 00:46:21.030 need to somehow be able to store them and analyze them. So there is also a lot of 00:46:21.030 --> 00:46:24.190 technology involved in the design of these detectors. 00:46:24.190 --> 00:46:30.060 Herald: Thank you. So the first question from microphone 2 in the middle. 00:46:30.060 --> 00:46:34.110 Microphone 2: So my question is. Herald: Please go close to the microphone. 00:46:34.110 --> 00:46:38.860 Microphone 2: My question is regarding the synchronization of the detector units when 00:46:38.860 --> 00:46:45.030 you're pointing to free electron laser so you can achieve this in synchronization. 00:46:45.030 --> 00:46:50.700 Caroline: This is also very complicated. It's easier to do in the lab. So you're 00:46:50.700 --> 00:46:54.800 talking about the synchronization of essentially the first pulse and the second 00:46:54.800 --> 00:47:00.540 pulse. Right. So in the lab, you typically generate the second pulse from part of the 00:47:00.540 --> 00:47:05.310 first pulse. So you have a very natural alignment, at least in time of these two 00:47:05.310 --> 00:47:10.190 pulses. The X-ray free electron lasers have special timing tools that allow you 00:47:10.190 --> 00:47:16.830 to find out how much is the time delay between your two pulses. But it's true 00:47:16.830 --> 00:47:21.520 that this is complicated to achieve and this limits the experimental time 00:47:21.520 --> 00:47:25.860 resolution to something that is even larger than the time duration of the 00:47:25.860 --> 00:47:30.540 pulses. Herald: So now next question from 00:47:30.540 --> 00:47:35.930 microphone number 3. Microphone 3: Yes, i remember in the 00:47:35.930 --> 00:47:42.810 beginning, you explained that your measuring method usually destroys your 00:47:42.810 --> 00:47:49.700 molecules. That's a bit of a contradiction to your idea to control. laughing 00:47:49.700 --> 00:47:59.090 Caroline: In principle, yes. But, so in the case of control, we would like to use 00:47:59.090 --> 00:48:04.650 a second pulse, that does not destroy the molecule. But for example, at least 00:48:04.650 --> 00:48:11.140 destroys it in a controlled way, for example. loud laughter So there's a difference between 00:48:11.140 --> 00:48:15.250 just blowing up your molecule and breaking apart a certain part, but yet that we are 00:48:15.250 --> 00:48:19.770 interested in. And that's what we would like to do in the control case. So we 00:48:19.770 --> 00:48:24.490 would like to be able to to control, for example, the fragmentation of a molecule 00:48:24.490 --> 00:48:30.230 such that we only get the important part out and everything else just goes away. 00:48:30.230 --> 00:48:34.430 Microphone3: Thank you. Herald: So then another question for 00:48:34.430 --> 00:48:39.240 microphone 2 in the middle. Microphone 2: So thank you for the talk. I 00:48:39.240 --> 00:48:44.470 was interested in how large structures or molecules can you imagine with this lab 00:48:44.470 --> 00:48:48.520 contributions and with this XFEL thing? Caroline: Sorry. Can you repeat? 00:48:48.520 --> 00:48:54.790 Microphone 2: So how large molecules can you imagine in this laboratory with this 00:48:54.790 --> 00:48:59.120 high harmonic measures? Caroline: So how large is not really the 00:48:59.120 --> 00:49:05.630 fundamental problem? People have taken snapshots of viruses or bigger bio 00:49:05.630 --> 00:49:11.870 molecules. If you want to - the problem is rather how small can we get? So yeah, to 00:49:11.870 --> 00:49:16.370 take pictures of a very small molecule. Currently we cannot take a picture of an 00:49:16.370 --> 00:49:21.780 individual small molecule, but what people do is they create crystals of a small 00:49:21.780 --> 00:49:25.260 molecule, sticking several of them together and then taking images of this 00:49:25.260 --> 00:49:30.371 whole crystal for single particles, I think right now about the scale of a virus 00:49:30.371 --> 00:49:34.180 nanometers. Microphone 2: Thank you. 00:49:34.180 --> 00:49:38.190 Herald: OK. Do we have another question from the Internet? 00:49:38.190 --> 00:49:43.740 Signal Angel: We have. So this is concerning your permanent destruction of 00:49:43.740 --> 00:49:49.450 forces, I guess. How do you isolate single atoms and molecules for analysing between 00:49:49.450 --> 00:49:55.860 the different exposures? Caroline: Yes, excellent question. So 00:49:55.860 --> 00:50:01.440 molecules can be made available in the gas phase by - so if you have them in a solid 00:50:01.440 --> 00:50:08.030 somewhere and you heat that up, they will evaporate from that surface. This is how 00:50:08.030 --> 00:50:11.760 you can get them in the gas phase. This, of course, assumes that you have a 00:50:11.760 --> 00:50:15.940 molecule, that is actually stable in the gas space, which is not true for all 00:50:15.940 --> 00:50:22.450 molecules. And then the, the hard thing is to align all three things. So the pump 00:50:22.450 --> 00:50:27.540 pulse, the probe pulse and the molecule all need to be there at the same time. 00:50:27.540 --> 00:50:32.600 There are people doing whole PhD theses on how to design gas nozzles that can provide 00:50:32.600 --> 00:50:37.670 this stream of molecules. Signal Angel: So you basically really 00:50:37.670 --> 00:50:41.190 having a stream coming from a nozzle? Caroline: Yes. 00:50:41.190 --> 00:50:43.300 Signal Angel: It's a very thin stream, I guess. 00:50:43.300 --> 00:50:47.620 Caroline: Yes. Signal Angel: Then you're exposing it like 00:50:47.620 --> 00:50:49.990 in a regular interval. Caroline: And of course you try to hit as 00:50:49.990 --> 00:50:56.180 many molecules as possible. So this is especially important when you do pictures 00:50:56.180 --> 00:50:59.760 of crystallized molecules because crystallizing these molecules is a lot of 00:50:59.760 --> 00:51:04.480 work. You don't want to waste like 99 percent that just fall away and you never 00:51:04.480 --> 00:51:07.990 take snapshots of them. Signal Angel: Thanks. 00:51:07.990 --> 00:51:10.990 Herald: So another question from microphone 3. 00:51:10.990 --> 00:51:18.810 Microphone 3: How do you construct this movie? I mean, for every pulse to have a 00:51:18.810 --> 00:51:25.240 new molecule and for every molecule is oriented differently in space and has 00:51:25.240 --> 00:51:31.190 different oscillation modes. How do correlate them? I mean, in the movie, I 00:51:31.190 --> 00:51:35.100 mean, every molecule is different than the previous one. 00:51:35.100 --> 00:51:41.400 Caroline: Yes. Excellent question. That's, So first, what people can do is align 00:51:41.400 --> 00:51:48.430 molecules. So especially molecules that are more or less linear. You can force 00:51:48.430 --> 00:51:55.410 them to be oriented in a certain way. And then there's also a bit of a secret in the 00:51:55.410 --> 00:52:00.220 trigger pulse that first sets off this motion. For example, if this trigger pulse 00:52:00.220 --> 00:52:04.600 is a very strong proto ionization, then this will kill off any sorts of 00:52:04.600 --> 00:52:08.910 vibrational states that you have had before in the molecule. So in this sense, 00:52:08.910 --> 00:52:12.840 the trigger parts really defines a time zero, that should be reproducible for any 00:52:12.840 --> 00:52:17.950 molecule that shows up in the stream and the rest is statistics. 00:52:17.950 --> 00:52:23.580 Microphone 3: Thank you. Herald: So there's another question on 00:52:23.580 --> 00:52:27.070 microphone 3. Microphone 3: Are there any pre pulses or 00:52:27.070 --> 00:52:31.420 ghosts, You need to get rid of? Caroline: Sorry. Again. 00:52:31.420 --> 00:52:36.550 Microphone 3: You have to control pre pulses or ghosts during this effect for 00:52:36.550 --> 00:52:39.560 measurement. Caroline: That I'm not really sure of, 00:52:39.560 --> 00:52:42.090 since I'm not really conducting experiments, but probably. 00:52:42.090 --> 00:52:49.510 Herald: And another one from the middle, from microphone 2 please. 00:52:49.510 --> 00:52:55.430 Microphone 2: I suppose if you apply for experimentation time at the XFEL laser, 00:52:55.430 --> 00:53:02.000 you have to submit very detailed plans and time lines and everything. And you will 00:53:02.000 --> 00:53:07.950 get the time window for your experiment, I guess. what's going to happen if you're 00:53:07.950 --> 00:53:13.760 not completely finished within that time window? Are they easy possibilities to 00:53:13.760 --> 00:53:19.180 extend the time or are they do they just say, well, you had your three weeks, 00:53:19.180 --> 00:53:23.120 you're out apply in 2026? Caroline: Yeah, I think it's a regular 00:53:23.120 --> 00:53:26.930 case, that you're not finished with your experiments by the time your beam time 00:53:26.930 --> 00:53:31.870 ends. That's how it usually goes. It's also unfortunatly not free weeks, but it's 00:53:31.870 --> 00:53:38.531 rather like 60 hours delivered in five shifts of twelve hours. So, yeah, you 00:53:38.531 --> 00:53:43.250 write a very detailed proposal of what you would like to do. Submit it to a panel of 00:53:43.250 --> 00:53:50.620 experts, both scientists and technicians. So they decide, is it interesting enough 00:53:50.620 --> 00:53:54.980 from a scientific point of view and is it feasible from a technical point of view? 00:53:54.980 --> 00:54:00.320 And then once you are there, you more or less set up your experiment and do as much 00:54:00.320 --> 00:54:06.490 as you can. If you want to come back, you need to submit an additional proposal. So, 00:54:06.490 --> 00:54:10.590 yeah, I think most experimental groups try to have several of these proposals running 00:54:10.590 --> 00:54:15.490 at the same time, so that there is not a two year delay between your data 00:54:15.490 --> 00:54:21.070 acquisition. But yes. No possibility to extend. It's booked already for the 00:54:21.070 --> 00:54:27.890 complete next year. The schedule is fixed. Herald: So I don't see any more people 00:54:27.890 --> 00:54:33.060 queuing up. If you want to pose a question, please do so now. In the 00:54:33.060 --> 00:54:35.460 meantime, I would ask the signal angel if there's another question from the 00:54:35.460 --> 00:54:38.240 Internet. Signal Angel: I have a question about the 00:54:38.240 --> 00:54:44.650 dimensions of all those machines. The undulator seems to be rather long and 00:54:44.650 --> 00:54:48.910 contain a lot of magnets. Do you have an idea how long it is and how many of those 00:54:48.910 --> 00:54:55.221 electromagnets are in there? Caroline: Yeah. Sorry, I didn't mention 00:54:55.221 --> 00:54:57.221 it. It's about, I think one hundred and seventy meters long in the case of the 00:54:57.221 --> 00:55:03.390 European XFEL. I'm not sure about the dimension of the individual magnets, but 00:55:03.390 --> 00:55:09.100 it's probably also in the hundreds of magnets, magnet pairs. 00:55:09.100 --> 00:55:16.080 Herald: So is there more - excuse me, there is a question on the microphone 00:55:16.080 --> 00:55:20.350 number 3. Microphone 3: Yeah. Hi. It's regarding the 00:55:20.350 --> 00:55:22.350 harmonic light. Herald: Please go closer to the 00:55:22.350 --> 00:55:24.350 microphone. Microphone 3: The harmonic light generator 00:55:24.350 --> 00:55:26.870 that you were showing at the very beginning, just before the one that won 00:55:26.870 --> 00:55:32.700 the Nobel Prize. And can you also produce light in the visible range? Or it has to 00:55:32.700 --> 00:55:38.050 be in the visible range? Caroline: The high harmonic generation? So 00:55:38.050 --> 00:55:43.160 in the in the visible range, you cannot create pulses that are so short that they 00:55:43.160 --> 00:55:50.960 would be interesting for what I'm doing. The pulse that comes in is already quite 00:55:50.960 --> 00:55:54.791 short. So it's already femtoseconds long. They just convert it into something that 00:55:54.791 --> 00:56:01.850 is fractions of a femtosecond long. And yeah. In the indivisible range that's kind 00:56:01.850 --> 00:56:05.800 of a limit how short your pulse can be. Microphone 3: So it is not a good 00:56:05.800 --> 00:56:11.060 candidate for hyperspectral light source. We need another kind of technique, I 00:56:11.060 --> 00:56:16.360 guess. Caroline: Well, I mean you are kind of 00:56:16.360 --> 00:56:21.070 limited what short pulses you can generate with which wavelength. 00:56:21.070 --> 00:56:26.441 Microphone 3: Thank you. Herald: So again, a question to the Signal 00:56:26.441 --> 00:56:29.020 Angel. Are there more questions from the internet? 00:56:29.020 --> 00:56:34.590 Signal Angel: Yes I have another one about the lifetime of the molecules in the beam? 00:56:34.590 --> 00:56:39.800 How fast are they degrading or how fast are they destructed? 00:56:39.800 --> 00:56:44.290 Caroline: So probably the question is about how fast they are destructed before 00:56:44.290 --> 00:56:53.420 - so either before our pulses hit the molecule the molecules should be stable 00:56:53.420 --> 00:56:57.330 enough to survive in the gas phase from the point where they are evaporated until 00:56:57.330 --> 00:57:01.370 the point where the pump and the probe pulse come together, because otherwise it 00:57:01.370 --> 00:57:07.560 doesn't make sense to study this molecule in the gas phase. When the probe pulse 00:57:07.560 --> 00:57:12.690 hits and it flows apart, I guess pico seconds until the whole molecules ... 00:57:12.690 --> 00:57:21.780 Microphone 3: Like instantaneous. Herald: So I don't see any more questions 00:57:21.780 --> 00:57:26.040 on the microphones and we have a few minutes left. So if there are more 00:57:26.040 --> 00:57:30.030 questions from the Internet, we can take maybe one or two more. 00:57:30.030 --> 00:57:42.560 Signal Angel: Give me a second. Herald: For people leaving already, please 00:57:42.560 --> 00:57:46.760 look if you have taken trash and bottles with you. 00:57:46.760 --> 00:57:52.390 Signal Angel: So this one very, very technical question. How do you compensate 00:57:52.390 --> 00:57:58.210 the electronic signal that the electronic signal reaction is probably slower than x 00:57:58.210 --> 00:58:05.240 ray or light or spectrum changes at one moment or at one particular moment, that 00:58:05.240 --> 00:58:10.170 was interesting to analyze. I do not understand the question, though. 00:58:10.170 --> 00:58:13.240 Caroline: I think I understand the question, but I don't have the answer 00:58:13.240 --> 00:58:16.860 because again, I'm a - that's the problem of speaking to a technical audience, you 00:58:16.860 --> 00:58:23.710 get a lot of these very technical question. Yeah. The data analysis is not 00:58:23.710 --> 00:58:32.810 instantaneous. So the data is transported somewhere safe in my imagination. And then 00:58:32.810 --> 00:58:37.180 taken from there. So this data analysis does not have to take place on the same 00:58:37.180 --> 00:58:41.330 timescale as the data acquisition, which I guess is also because of the problem that 00:58:41.330 --> 00:58:47.530 was mentioned in the question. Herald: I might interrupt here. Maybe it's 00:58:47.530 --> 00:58:55.680 also about the signal transmission, like the signal rising of the signal of the 00:58:55.680 --> 00:59:01.900 electrical signal transmissions. Because this would probably require bandwidths of 00:59:01.900 --> 00:59:06.680 several megahertz, gigahertz, I don't know, to transport these very fast 00:59:06.680 --> 00:59:09.680 results. Caroline: Yeah. I think that's also a 00:59:09.680 --> 00:59:15.130 problem of constructing the right detector. That has been solved apparently 00:59:15.130 --> 00:59:20.690 because they can take these images. laughter Herald: And on the other hand, we have 10 00:59:20.690 --> 00:59:28.760 gigabit either nets. So we get faster and faster electronics. More questions from 00:59:28.760 --> 00:59:39.921 the Internet? Does not look like it, also the time is running out. So let's thank 00:59:39.921 --> 00:59:42.760 Caroline for her marvelous talk. Applause, final music 00:59:42.760 --> 01:00:13.000 subtitles created by c3subtitles.de in the year 2019. Join, and help us!