WEBVTT

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(English captions by Andrea Matsumoto, University of Michigan.)

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Prepare sputum smear by taking the sputum
from the sputum container using the loop,

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which has been sterilized.

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Spread it on the microscope slide very wide,
about two to three centimeter diameter and

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then flame the loop again.

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Flame the sputum smear by fixing it.

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These are the spirit lamp and then the stains
required for the staining procedure.

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Cover the smear with Carbol Fuchsin.

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And heat it with the spirit lamp intermittently
until it steams but should not boil or the

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steam should not get dried and should be done
intermittently for five minutes.

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Tip the steam off and allow it to cool before
washing the excess stain from the slide of

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the smear.

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Cover the smear with the decolorizer is twenty
percent sulfuric acid for two minutes.

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After two minutes tip off the decolorizer
and wash it under the tap water or distilled

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water.

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Cover the smear with the counterstain with
methylene blue for two minutes.

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No heating.

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Wash it under the tap water or distilled water.

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And air dry it on the draining rack.

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Put oil immersion on the smeared slide and
then examine it under the times hundred (100X) objective

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of the microscope.

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And you are expected to see reddish or pinkish
bacilli, which can appear in a form of a cocci

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or it can also appear as a spiral or it can
appear as a dot.