[Script Info]
Title: 
[Events]
Format: Layer, Start, End, Style, Name, MarginL, MarginR, MarginV, Effect, Text
Dialogue: 0,0:00:04.00,0:00:08.00,Default,,0000,0000,0000,,(English captions by Andrea Matsumoto, University of Michigan.)
Dialogue: 0,0:00:15.00,0:00:25.00,Default,,0000,0000,0000,,Prepare sputum smear by taking the sputum\Nfrom the sputum container using the loop,
Dialogue: 0,0:00:25.00,0:00:30.00,Default,,0000,0000,0000,,which has been sterilized.
Dialogue: 0,0:00:30.00,0:00:39.00,Default,,0000,0000,0000,,Spread it on the microscope slide very wide,\Nabout two to three centimeter diameter and
Dialogue: 0,0:00:39.00,0:00:42.00,Default,,0000,0000,0000,,then flame the loop again.
Dialogue: 0,0:00:42.00,0:00:52.00,Default,,0000,0000,0000,,Flame the sputum smear by fixing it.
Dialogue: 0,0:00:52.00,0:01:11.00,Default,,0000,0000,0000,,These are the spirit lamp and then the stains\Nrequired for the staining procedure.
Dialogue: 0,0:01:11.00,0:01:27.00,Default,,0000,0000,0000,,Cover the smear with Carbol Fuchsin.
Dialogue: 0,0:01:27.00,0:01:35.00,Default,,0000,0000,0000,,And heat it with the spirit lamp intermittently\Nuntil it steams but should not boil or the
Dialogue: 0,0:01:35.00,0:02:03.00,Default,,0000,0000,0000,,steam should not get dried and should be done\Nintermittently for five minutes.
Dialogue: 0,0:02:03.00,0:02:13.00,Default,,0000,0000,0000,,Tip the steam off and allow it to cool before\Nwashing the excess stain from the slide of
Dialogue: 0,0:02:13.00,0:02:21.00,Default,,0000,0000,0000,,the smear.
Dialogue: 0,0:02:21.00,0:02:46.00,Default,,0000,0000,0000,,Cover the smear with the decolorizer is twenty\Npercent sulfuric acid for two minutes.
Dialogue: 0,0:02:46.00,0:02:55.00,Default,,0000,0000,0000,,After two minutes tip off the decolorizer\Nand wash it under the tap water or distilled
Dialogue: 0,0:02:55.00,0:03:05.00,Default,,0000,0000,0000,,water.
Dialogue: 0,0:03:05.00,0:03:15.00,Default,,0000,0000,0000,,Cover the smear with the counterstain with\Nmethylene blue for two minutes.
Dialogue: 0,0:03:15.00,0:03:21.00,Default,,0000,0000,0000,,No heating.
Dialogue: 0,0:03:21.00,0:03:29.00,Default,,0000,0000,0000,,Wash it under the tap water or distilled water.
Dialogue: 0,0:03:29.00,0:03:39.00,Default,,0000,0000,0000,,And air dry it on the draining rack.
Dialogue: 0,0:03:39.00,0:03:50.00,Default,,0000,0000,0000,,Put oil immersion on the smeared slide and\Nthen examine it under the times hundred (100X) objective
Dialogue: 0,0:03:50.00,0:03:52.00,Default,,0000,0000,0000,,of the microscope.
Dialogue: 0,0:03:52.00,0:04:00.00,Default,,0000,0000,0000,,And you are expected to see reddish or pinkish\Nbacilli, which can appear in a form of a cocci
Dialogue: 0,0:04:00.00,0:04:04.00,Default,,0000,0000,0000,,or it can also appear as a spiral or it can\Nappear as a dot.