(English captions by Andrea Matsumoto, University of Michigan.)
Take venous blood sample into a sequestrated air bottle mixed very well.
Take a drop onto a microscope slide.
We can also as well take a finger prick from the third or ring finger.
Using a microscope slide as a spreader, spread the blood, drop of blood, into a thin form.
Air-dry the smear, the glass smear on a draining rack.
Prepare a thick smear by putting blood from a sequestrated bottle onto the microscope
slide or as well you can use blood from a finger prick.
Using a microscope slide spread the blood out.
Allow it to air dry on the draining rack or in the incubator.
Field's Rapid Staining for Malaria:
Stain the thick smear in a field stain A for five seconds.
And this dehemoglobinizes the red blood cells.
Wash under the tap water or buffered water.
Put it in field stain B for five seconds.
After which you will wash under the tap water or with buffered water.
Air-dry it on the draining rack.
Fix the thin smear in methanol for two seconds.
Allow it to air dry and put it in field stain B for six seconds.
After which you wash under tap water or buffered water.
Put it in field stain A for six seconds.
After which you wash under the tap water to get rid of the excess stain and this stain
is called river staining and this preserve the red blood cells.
The Giemsa Stain:
Stain the thick smear in one in ten dilution of Giemsa for five to fifteen minutes.
Wash the excess stain under tap water or buffered water.
The thin smear is fixed in methanol for two seconds after which it is put in the Giemsa
one in ten dilution for five to fifteen minutes.
Wash off the excess stain under tap water or using buffered water and air dry it on
draining rack.
Examine the thin slides under times hundred (100x) using oil immersion.
It is better to see for the thick film the parasites and the white blood cells.
The parasites will look like a ring form or dots, which appear in pairs.
But in a thin film the parasites are found inside the red blood cell for which we can
use to determine the species of the parasites.