WEBVTT

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(English captions by Andrea Matsumoto, University of Michigan.)

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Take venous blood sample into a sequestrated
air bottle mixed very well.

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Take a drop onto a microscope slide.

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We can also as well take a finger prick from
the third or ring finger.

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Using a microscope slide as a spreader, spread
the blood, drop of blood, into a thin form.

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Air-dry the smear, the glass smear on a draining
rack.

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Prepare a thick smear by putting blood from
a sequestrated bottle onto the microscope

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slide or as well you can use blood from a
finger prick.

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Using a microscope slide spread the blood
out.

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Allow it to air dry on the draining rack or
in the incubator.

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Field's Rapid Staining for Malaria:

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Stain the thick smear in a field stain A for
five seconds.

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And this dehemoglobinizes the red blood cells.

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Wash under the tap water or buffered water.

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Put it in field stain B for five seconds.

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After which you will wash under the tap water
or with buffered water.

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Air-dry it on the draining rack.

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Fix the thin smear in methanol for two seconds.

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Allow it to air dry and put it in field stain
B for six seconds.

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After which you wash under tap water or buffered
water.

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Put it in field stain A for six seconds.

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After which you wash under the tap water to
get rid of the excess stain and this stain

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is called river staining and this preserve
the red blood cells.

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The Giemsa Stain:

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Stain the thick smear in one in ten dilution
of Giemsa for five to fifteen minutes.

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Wash the excess stain under tap water or buffered
water.

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The thin smear is fixed in methanol for two
seconds after which it is put in the Giemsa

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one in ten dilution for five to fifteen minutes.

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Wash off the excess stain under tap water
or using buffered water and air dry it on

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draining rack.

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Examine the thin slides under times hundred
(100x) using oil immersion.

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It is better to see for the thick film the
parasites and the white blood cells.

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The parasites will look like a ring form or
dots, which appear in pairs.

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But in a thin film the parasites are found
inside the red blood cell for which we can

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use to determine the species of the parasites.