WEBVTT
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you may be wondering what your doing at
the front of a bioengineering madam i a
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total strangers talking to you right now
but i did talk about some of the on some
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experiments that herbert boyer and
stanley commented in the nation
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you might be thinking to yourself
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uh... the seventies in america when the
dinosaurs lived
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boring
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well had never gotten a vaccine are
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groceries record he started
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that complex to some of the experiments
that these two had
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halsted setting
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where they accept and values that cut
dat label to in three days
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continue bacterial dna_
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the hanging at the field of genetic
engineering and thanks to all the work
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that they did
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we have medications for what that means
that protective
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you know i exist on the inside
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so the first thing that clinton boy it
did was they checked dna_ from driving
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headed up
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as you might think you guys that's going
on in this to you
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here some dna_ from the front
encouraging traits that affect how the
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front develops had survived
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now there's a piece that dna_
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in this genome whose job listing code
for certain proteins that the front uses
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to survive
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we're gonna cut this peace out using a
restriction enzyme
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this is the thing that way discovered
its
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kind of like a pair of scissors
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cutting the dna_ act
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specific points we can get different
pieces
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now there are times are different kinds
restriction enzymes and they'll come in
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different places in dna_
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are and send their resume tuesday is
called echo are one
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now this piece but dna_ has more than
one spot
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that echo are one can cut as shown by
the dot lines
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so we're going to end up with other
pieces of dna_ in addition to the one
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we actually want
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now that's actually ok because
articulate that probably did
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what's going on in the two is really
really discarding after pieces dna_'s
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just like it
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now here's a tip
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plasmid
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basically circulating of dna_ that can
exist in bacteria any basically impose
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for certain traits that affect how
bacteria look actor civilian anti at our
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dna_ from the frog dna_ try
bacterial dna_
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we need to cut the president clinton
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so we've got to add are echo are one
scissors
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and they're going to cut
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at the site
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you let this reaction proceeds letting
all the ingredients the classmates
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restriction enzyme and somewhat
interesting at cnn_ tel all the
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plastic cup like that's
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now we need at the dna_ please
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and allow the pieces to joint
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here's what's going on in the to camp
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i've got pieces earth
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try to dna_
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and i had cracked cut classmates like
this
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i need to ad
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the sky
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into this president
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and the way and they do that
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is the eddie an enzyme called dna_
like case
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this enzyme tenet acts like teak and it
fixes the dna_ in six th
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into the test name
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nine other important thing at that
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the present itself is that it resent
marker than a series of situation in
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haiti antibiotic
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contact recycling
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this will come in handy in a little bit
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after a little while level couple types
of dna_ going on
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the people non
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but will come from it
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you know
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guardsmen classmates that just caused a
mom
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at the
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and seat
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thing about these guys is that they'll
actually die if you bad detect recycling
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antibiotic which we talked about bailey
it to them
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webinar invite you to get the class and
inside a vehicle and with that because
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all the cells down and then quickly he
did them up
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creating little holes
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and the cell membrane
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then the president's concert through
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and get into the south
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these are the kind of bacteria that we
have now
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bacteria
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that got the practically
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but you also get bacteria that cut
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the president's that
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we get one
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say
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this one with
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the wrong piece of dna_ way
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this i was just a plane parts that
without any frightening
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more than likely will
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get ones that just didn't pick up any of
the president's no end in eighteen x
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display t_-cells on some tight recycling
on their plates which is basically some
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fancy jello that has antibiotic in it
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so these guys won't be able to grow
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but these guys well
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plated
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people live
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transformed this tiny plasmids
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onto this plate which has kept recycling
it
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have likened that what they're saying
they need to be here overnight and allow
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our bacteria to grow
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watered down in a game in the morning
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said he didn't have
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definitely got some colonies regularly
remain
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cell not a problem that we have
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is we need everyone distinguish that
actually had them at these primates
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from the one
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that night
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vis a man who don't have an idea demand
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resulting famine deal
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this is the same techniques that we did
at the very beginning of this
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maintenance enemies back
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smith has been spent on these bacteria
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then reading is the same restriction
enzymes
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you might be able to notice
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dot proponents say
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is a different sizes then that means
that comes in the background
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surrounding areas isn't something larger
belmont
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needs a technique adela electrophoresis
and let's get it is it's gonna separate
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are different pieces of dna_ half
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and from this experiment will be able to
tell
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the president's that took up the
frightening
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from the ones that did it
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bought from the ones that just closed up
on themselves
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deflating techniques that i don't know
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feel their lives
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galina being made
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very faint
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well i thought that i printed a gag
thanks for stopping by
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of the american aircon she'd seen or
some of the cloning techniques he's not
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today
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disheveled and they say the internet
engineering
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ta waiters
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k