[Script Info]
Title: 
[Events]
Format: Layer, Start, End, Style, Name, MarginL, MarginR, MarginV, Effect, Text
Dialogue: 0,0:00:10.47,0:00:14.09,Default,,0000,0000,0000,,you may be wondering what your doing at\Nthe front of a bioengineering madam i a
Dialogue: 0,0:00:14.09,0:00:18.35,Default,,0000,0000,0000,,total strangers talking to you right now\Nbut i did talk about some of the on some
Dialogue: 0,0:00:18.35,0:00:22.79,Default,,0000,0000,0000,,experiments that herbert boyer and\Nstanley commented in the nation
Dialogue: 0,0:00:22.79,0:00:24.82,Default,,0000,0000,0000,,you might be thinking to yourself
Dialogue: 0,0:00:24.82,0:00:29.30,Default,,0000,0000,0000,,uh... the seventies in america when the\Ndinosaurs lived
Dialogue: 0,0:00:29.30,0:00:30.28,Default,,0000,0000,0000,,boring
Dialogue: 0,0:00:30.28,0:00:32.96,Default,,0000,0000,0000,,well had never gotten a vaccine are
Dialogue: 0,0:00:32.96,0:00:35.29,Default,,0000,0000,0000,,groceries record he started
Dialogue: 0,0:00:35.29,0:00:38.25,Default,,0000,0000,0000,,that complex to some of the experiments\Nthat these two had
Dialogue: 0,0:00:38.25,0:00:40.05,Default,,0000,0000,0000,,halsted setting
Dialogue: 0,0:00:40.05,0:00:44.62,Default,,0000,0000,0000,,where they accept and values that cut\Ndat label to in three days
Dialogue: 0,0:00:44.62,0:00:46.39,Default,,0000,0000,0000,,continue bacterial d{\u1}n{\u0}a_
Dialogue: 0,0:00:46.39,0:00:49.84,Default,,0000,0000,0000,,the hanging at the field of genetic\Nengineering and thanks to all the work
Dialogue: 0,0:00:49.84,0:00:51.03,Default,,0000,0000,0000,,that they did
Dialogue: 0,0:00:51.03,0:00:58.03,Default,,0000,0000,0000,,we have medications for what that means\Nthat protective
Dialogue: 0,0:00:58.32,0:01:01.36,Default,,0000,0000,0000,,you know i exist on the inside
Dialogue: 0,0:01:01.36,0:01:06.51,Default,,0000,0000,0000,,so the first thing that clinton boy it\Ndid was they checked d{\u1}n{\u0}a_ from driving
Dialogue: 0,0:01:06.51,0:01:08.96,Default,,0000,0000,0000,,headed up
Dialogue: 0,0:01:08.96,0:01:11.73,Default,,0000,0000,0000,,as you might think you guys that's going\Non in this to you
Dialogue: 0,0:01:11.73,0:01:15.81,Default,,0000,0000,0000,,here some d{\u1}n{\u0}a_ from the front\Nencouraging traits that affect how the
Dialogue: 0,0:01:15.81,0:01:19.01,Default,,0000,0000,0000,,front develops had survived
Dialogue: 0,0:01:19.01,0:01:21.35,Default,,0000,0000,0000,,now there's a piece that d{\u1}n{\u0}a_
Dialogue: 0,0:01:21.35,0:01:25.65,Default,,0000,0000,0000,,in this genome whose job listing code\Nfor certain proteins that the front uses
Dialogue: 0,0:01:25.65,0:01:26.90,Default,,0000,0000,0000,,to survive
Dialogue: 0,0:01:26.90,0:01:30.32,Default,,0000,0000,0000,,we're gonna cut this peace out using a\Nrestriction enzyme
Dialogue: 0,0:01:30.32,0:01:32.54,Default,,0000,0000,0000,,this is the thing that way discovered\Nits
Dialogue: 0,0:01:32.54,0:01:34.88,Default,,0000,0000,0000,,kind of like a pair of scissors
Dialogue: 0,0:01:34.88,0:01:36.13,Default,,0000,0000,0000,,cutting the d{\u1}n{\u0}a_ act
Dialogue: 0,0:01:36.13,0:01:38.96,Default,,0000,0000,0000,,specific points we can get different\Npieces
Dialogue: 0,0:01:38.96,0:01:42.16,Default,,0000,0000,0000,,now there are times are different kinds\Nrestriction enzymes and they'll come in
Dialogue: 0,0:01:42.16,0:01:44.09,Default,,0000,0000,0000,,different places in d{\u1}n{\u0}a_
Dialogue: 0,0:01:44.09,0:01:47.45,Default,,0000,0000,0000,,are and send their resume tuesday is\Ncalled echo are one
Dialogue: 0,0:01:47.45,0:01:50.31,Default,,0000,0000,0000,,now this piece but d{\u1}n{\u0}a_ has more than\None spot
Dialogue: 0,0:01:50.31,0:01:53.73,Default,,0000,0000,0000,,that echo are one can cut as shown by\Nthe dot lines
Dialogue: 0,0:01:53.73,0:01:57.01,Default,,0000,0000,0000,,so we're going to end up with other\Npieces of d{\u1}n{\u0}a_ in addition to the one
Dialogue: 0,0:01:57.01,0:01:58.29,Default,,0000,0000,0000,,we actually want
Dialogue: 0,0:01:58.29,0:02:01.73,Default,,0000,0000,0000,,now that's actually ok because\Narticulate that probably did
Dialogue: 0,0:02:01.73,0:02:05.71,Default,,0000,0000,0000,,what's going on in the two is really\Nreally discarding after pieces d{\u1}n{\u0}a_'s
Dialogue: 0,0:02:05.71,0:02:12.71,Default,,0000,0000,0000,,just like it
Dialogue: 0,0:02:12.89,0:02:14.13,Default,,0000,0000,0000,,now here's a tip
Dialogue: 0,0:02:14.13,0:02:15.60,Default,,0000,0000,0000,,plasmid
Dialogue: 0,0:02:15.60,0:02:20.40,Default,,0000,0000,0000,,basically circulating of d{\u1}n{\u0}a_ that can\Nexist in bacteria any basically impose
Dialogue: 0,0:02:20.40,0:02:25.100,Default,,0000,0000,0000,,for certain traits that affect how\Nbacteria look actor civilian anti at our
Dialogue: 0,0:02:25.100,0:02:29.64,Default,,0000,0000,0000,,d{\u1}n{\u0}a_ from the frog d{\u1}n{\u0}a_ try\Nbacterial d{\u1}n{\u0}a_
Dialogue: 0,0:02:29.64,0:02:31.86,Default,,0000,0000,0000,,we need to cut the president clinton
Dialogue: 0,0:02:31.86,0:02:35.53,Default,,0000,0000,0000,,so we've got to add are echo are one\Nscissors
Dialogue: 0,0:02:35.53,0:02:37.20,Default,,0000,0000,0000,,and they're going to cut
Dialogue: 0,0:02:37.20,0:02:41.57,Default,,0000,0000,0000,,at the site
Dialogue: 0,0:02:41.57,0:02:45.44,Default,,0000,0000,0000,,you let this reaction proceeds letting\Nall the ingredients the classmates
Dialogue: 0,0:02:45.44,0:02:49.38,Default,,0000,0000,0000,,restriction enzyme and somewhat\Ninteresting at c{\u1}n{\u0}n_ tel all the
Dialogue: 0,0:02:49.38,0:02:51.05,Default,,0000,0000,0000,,plastic cup like that's
Dialogue: 0,0:02:51.05,0:02:53.91,Default,,0000,0000,0000,,now we need at the d{\u1}n{\u0}a_ please
Dialogue: 0,0:02:53.91,0:02:57.92,Default,,0000,0000,0000,,and allow the pieces to joint
Dialogue: 0,0:02:57.92,0:02:59.95,Default,,0000,0000,0000,,here's what's going on in the to camp
Dialogue: 0,0:02:59.95,0:03:01.23,Default,,0000,0000,0000,,i've got pieces earth
Dialogue: 0,0:03:01.23,0:03:03.79,Default,,0000,0000,0000,,try to d{\u1}n{\u0}a_
Dialogue: 0,0:03:03.79,0:03:08.49,Default,,0000,0000,0000,,and i had cracked cut classmates like\Nthis
Dialogue: 0,0:03:08.49,0:03:10.18,Default,,0000,0000,0000,,i need to ad
Dialogue: 0,0:03:10.18,0:03:11.39,Default,,0000,0000,0000,,the sky
Dialogue: 0,0:03:11.39,0:03:13.08,Default,,0000,0000,0000,,into this president
Dialogue: 0,0:03:13.08,0:03:14.56,Default,,0000,0000,0000,,and the way and they do that
Dialogue: 0,0:03:14.56,0:03:18.24,Default,,0000,0000,0000,,is the eddie an enzyme called d{\u1}n{\u0}a_\Nlike case
Dialogue: 0,0:03:18.24,0:03:22.60,Default,,0000,0000,0000,,this enzyme tenet acts like teak and it\Nfixes the d{\u1}n{\u0}a_ in six th
Dialogue: 0,0:03:22.60,0:03:27.11,Default,,0000,0000,0000,,into the test name
Dialogue: 0,0:03:27.11,0:03:29.30,Default,,0000,0000,0000,,nine other important thing at that
Dialogue: 0,0:03:29.30,0:03:33.66,Default,,0000,0000,0000,,the present itself is that it resent\Nmarker than a series of situation in
Dialogue: 0,0:03:33.66,0:03:35.02,Default,,0000,0000,0000,,haiti antibiotic
Dialogue: 0,0:03:35.02,0:03:36.50,Default,,0000,0000,0000,,contact recycling
Dialogue: 0,0:03:36.50,0:03:38.52,Default,,0000,0000,0000,,this will come in handy in a little bit
Dialogue: 0,0:03:38.52,0:03:45.52,Default,,0000,0000,0000,,after a little while level couple types\Nof d{\u1}n{\u0}a_ going on
Dialogue: 0,0:03:45.68,0:03:48.50,Default,,0000,0000,0000,,the people non
Dialogue: 0,0:03:48.50,0:03:54.13,Default,,0000,0000,0000,,but will come from it
Dialogue: 0,0:03:54.13,0:03:56.93,Default,,0000,0000,0000,,you know
Dialogue: 0,0:03:56.93,0:04:01.22,Default,,0000,0000,0000,,guardsmen classmates that just caused a\Nmom
Dialogue: 0,0:04:01.22,0:04:07.41,Default,,0000,0000,0000,,at the
Dialogue: 0,0:04:07.41,0:04:10.50,Default,,0000,0000,0000,,and seat
Dialogue: 0,0:04:10.50,0:04:15.17,Default,,0000,0000,0000,,thing about these guys is that they'll\Nactually die if you bad detect recycling
Dialogue: 0,0:04:15.17,0:04:19.55,Default,,0000,0000,0000,,antibiotic which we talked about bailey\Nit to them
Dialogue: 0,0:04:19.55,0:04:24.02,Default,,0000,0000,0000,,webinar invite you to get the class and\Ninside a vehicle and with that because
Dialogue: 0,0:04:24.02,0:04:27.48,Default,,0000,0000,0000,,all the cells down and then quickly he\Ndid them up
Dialogue: 0,0:04:27.48,0:04:29.27,Default,,0000,0000,0000,,creating little holes
Dialogue: 0,0:04:29.27,0:04:30.86,Default,,0000,0000,0000,,and the cell membrane
Dialogue: 0,0:04:30.86,0:04:36.01,Default,,0000,0000,0000,,then the president's concert through
Dialogue: 0,0:04:36.01,0:04:37.51,Default,,0000,0000,0000,,and get into the south
Dialogue: 0,0:04:37.51,0:04:40.38,Default,,0000,0000,0000,,these are the kind of bacteria that we\Nhave now
Dialogue: 0,0:04:40.38,0:04:41.36,Default,,0000,0000,0000,,bacteria
Dialogue: 0,0:04:41.36,0:04:45.57,Default,,0000,0000,0000,,that got the practically
Dialogue: 0,0:04:45.57,0:04:48.22,Default,,0000,0000,0000,,but you also get bacteria that cut
Dialogue: 0,0:04:48.22,0:04:49.63,Default,,0000,0000,0000,,the president's that
Dialogue: 0,0:04:49.63,0:04:50.57,Default,,0000,0000,0000,,we get one
Dialogue: 0,0:04:50.57,0:04:51.77,Default,,0000,0000,0000,,say
Dialogue: 0,0:04:51.77,0:04:52.68,Default,,0000,0000,0000,,this one with
Dialogue: 0,0:04:52.68,0:04:54.89,Default,,0000,0000,0000,,the wrong piece of d{\u1}n{\u0}a_ way
Dialogue: 0,0:04:54.89,0:04:58.99,Default,,0000,0000,0000,,this i was just a plane parts that\Nwithout any frightening
Dialogue: 0,0:04:58.99,0:05:01.03,Default,,0000,0000,0000,,more than likely will
Dialogue: 0,0:05:01.03,0:05:04.57,Default,,0000,0000,0000,,get ones that just didn't pick up any of\Nthe president's no end in eighteen x
Dialogue: 0,0:05:04.57,0:05:08.62,Default,,0000,0000,0000,,display t_-cells on some tight recycling\Non their plates which is basically some
Dialogue: 0,0:05:08.62,0:05:11.29,Default,,0000,0000,0000,,fancy jello that has antibiotic in it
Dialogue: 0,0:05:11.29,0:05:14.68,Default,,0000,0000,0000,,so these guys won't be able to grow
Dialogue: 0,0:05:14.68,0:05:16.91,Default,,0000,0000,0000,,but these guys well
Dialogue: 0,0:05:16.91,0:05:17.96,Default,,0000,0000,0000,,plated
Dialogue: 0,0:05:17.96,0:05:20.23,Default,,0000,0000,0000,,people live
Dialogue: 0,0:05:20.23,0:05:22.37,Default,,0000,0000,0000,,transformed this tiny plasmids
Dialogue: 0,0:05:22.37,0:05:25.50,Default,,0000,0000,0000,,onto this plate which has kept recycling\Nit
Dialogue: 0,0:05:25.50,0:05:28.99,Default,,0000,0000,0000,,have likened that what they're saying\Nthey need to be here overnight and allow
Dialogue: 0,0:05:28.99,0:05:30.37,Default,,0000,0000,0000,,our bacteria to grow
Dialogue: 0,0:05:30.37,0:05:37.37,Default,,0000,0000,0000,,watered down in a game in the morning
Dialogue: 0,0:05:37.42,0:05:39.02,Default,,0000,0000,0000,,said he didn't have
Dialogue: 0,0:05:39.02,0:05:42.52,Default,,0000,0000,0000,,definitely got some colonies regularly\Nremain
Dialogue: 0,0:05:42.52,0:05:44.75,Default,,0000,0000,0000,,cell not a problem that we have
Dialogue: 0,0:05:44.75,0:05:49.42,Default,,0000,0000,0000,,is we need everyone distinguish that\Nactually had them at these primates
Dialogue: 0,0:05:49.42,0:05:50.73,Default,,0000,0000,0000,,from the one
Dialogue: 0,0:05:50.73,0:05:52.03,Default,,0000,0000,0000,,that night
Dialogue: 0,0:05:52.03,0:05:55.01,Default,,0000,0000,0000,,vis a man who don't have an idea demand
Dialogue: 0,0:05:55.01,0:05:56.51,Default,,0000,0000,0000,,resulting famine deal
Dialogue: 0,0:05:56.51,0:06:00.36,Default,,0000,0000,0000,,this is the same techniques that we did\Nat the very beginning of this
Dialogue: 0,0:06:00.36,0:06:02.01,Default,,0000,0000,0000,,maintenance enemies back
Dialogue: 0,0:06:02.01,0:06:05.17,Default,,0000,0000,0000,,smith has been spent on these bacteria
Dialogue: 0,0:06:05.17,0:06:10.65,Default,,0000,0000,0000,,then reading is the same restriction\Nenzymes
Dialogue: 0,0:06:10.65,0:06:12.49,Default,,0000,0000,0000,,you might be able to notice
Dialogue: 0,0:06:12.49,0:06:14.21,Default,,0000,0000,0000,,dot proponents say
Dialogue: 0,0:06:14.21,0:06:18.73,Default,,0000,0000,0000,,is a different sizes then that means\Nthat comes in the background
Dialogue: 0,0:06:18.73,0:06:22.63,Default,,0000,0000,0000,,surrounding areas isn't something larger\Nbelmont
Dialogue: 0,0:06:22.63,0:06:27.32,Default,,0000,0000,0000,,needs a technique adela electrophoresis\Nand let's get it is it's gonna separate
Dialogue: 0,0:06:27.32,0:06:29.81,Default,,0000,0000,0000,,are different pieces of d{\u1}n{\u0}a_ half
Dialogue: 0,0:06:29.81,0:06:32.97,Default,,0000,0000,0000,,and from this experiment will be able to\Ntell
Dialogue: 0,0:06:32.97,0:06:35.60,Default,,0000,0000,0000,,the president's that took up the\Nfrightening
Dialogue: 0,0:06:35.60,0:06:37.17,Default,,0000,0000,0000,,from the ones that did it
Dialogue: 0,0:06:37.17,0:06:44.17,Default,,0000,0000,0000,,bought from the ones that just closed up\Non themselves
Dialogue: 0,0:06:44.64,0:06:47.71,Default,,0000,0000,0000,,deflating techniques that i don't know
Dialogue: 0,0:06:47.71,0:06:51.46,Default,,0000,0000,0000,,feel their lives
Dialogue: 0,0:06:51.46,0:06:54.18,Default,,0000,0000,0000,,galina being made
Dialogue: 0,0:06:54.18,0:06:56.11,Default,,0000,0000,0000,,very faint
Dialogue: 0,0:06:56.11,0:06:59.50,Default,,0000,0000,0000,,well i thought that i printed a gag\Nthanks for stopping by
Dialogue: 0,0:06:59.50,0:07:03.27,Default,,0000,0000,0000,,of the american aircon she'd seen or\Nsome of the cloning techniques he's not
Dialogue: 0,0:07:03.27,0:07:04.49,Default,,0000,0000,0000,,today
Dialogue: 0,0:07:04.49,0:07:07.15,Default,,0000,0000,0000,,disheveled and they say the internet\Nengineering
Dialogue: 0,0:07:07.15,0:07:14.13,Default,,0000,0000,0000,,ta waiters
Dialogue: 0,0:07:14.13,0:07:15.07,Default,,0000,0000,0000,,k