0:00:10.469,0:00:14.089
you may be wondering what your doing at[br]the front of a bioengineering madam i a
0:00:14.089,0:00:18.349
total strangers talking to you right now[br]but i did talk about some of the on some
0:00:18.349,0:00:22.789
experiments that herbert boyer and[br]stanley commented in the nation
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you might be thinking to yourself
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uh... the seventies in america when the[br]dinosaurs lived
0:00:29.300,0:00:30.279
boring
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well had never gotten a vaccine are
0:00:32.960,0:00:35.290
groceries record he started
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that complex to some of the experiments[br]that these two had
0:00:38.250,0:00:40.050
halsted setting
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where they accept and values that cut[br]dat label to in three days
0:00:44.620,0:00:46.390
continue bacterial dna_
0:00:46.390,0:00:49.840
the hanging at the field of genetic[br]engineering and thanks to all the work
0:00:49.840,0:00:51.030
that they did
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we have medications for what that means[br]that protective
0:00:58.320,0:01:01.360
you know i exist on the inside
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so the first thing that clinton boy it[br]did was they checked dna_ from driving
0:01:06.510,0:01:08.960
headed up
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as you might think you guys that's going[br]on in this to you
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here some dna_ from the front[br]encouraging traits that affect how the
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front develops had survived
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now there's a piece that dna_
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in this genome whose job listing code[br]for certain proteins that the front uses
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to survive
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we're gonna cut this peace out using a[br]restriction enzyme
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this is the thing that way discovered[br]its
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kind of like a pair of scissors
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cutting the dna_ act
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specific points we can get different[br]pieces
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now there are times are different kinds[br]restriction enzymes and they'll come in
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different places in dna_
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are and send their resume tuesday is[br]called echo are one
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now this piece but dna_ has more than[br]one spot
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that echo are one can cut as shown by[br]the dot lines
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so we're going to end up with other[br]pieces of dna_ in addition to the one
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we actually want
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now that's actually ok because[br]articulate that probably did
0:02:01.729,0:02:05.710
what's going on in the two is really[br]really discarding after pieces dna_'s
0:02:05.710,0:02:12.710
just like it
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now here's a tip
0:02:14.129,0:02:15.599
plasmid
0:02:15.599,0:02:20.400
basically circulating of dna_ that can[br]exist in bacteria any basically impose
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for certain traits that affect how[br]bacteria look actor civilian anti at our
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dna_ from the frog dna_ try[br]bacterial dna_
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we need to cut the president clinton
0:02:31.859,0:02:35.529
so we've got to add are echo are one[br]scissors
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and they're going to cut
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at the site
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you let this reaction proceeds letting[br]all the ingredients the classmates
0:02:45.439,0:02:49.379
restriction enzyme and somewhat[br]interesting at cnn_ tel all the
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plastic cup like that's
0:02:51.049,0:02:53.909
now we need at the dna_ please
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and allow the pieces to joint
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here's what's going on in the to camp
0:02:59.950,0:03:01.230
i've got pieces earth
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try to dna_
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and i had cracked cut classmates like[br]this
0:03:08.489,0:03:10.180
i need to ad
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the sky
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into this president
0:03:13.079,0:03:14.560
and the way and they do that
0:03:14.560,0:03:18.239
is the eddie an enzyme called dna_[br]like case
0:03:18.239,0:03:22.599
this enzyme tenet acts like teak and it[br]fixes the dna_ in six th
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into the test name
0:03:27.109,0:03:29.299
nine other important thing at that
0:03:29.299,0:03:33.659
the present itself is that it resent[br]marker than a series of situation in
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haiti antibiotic
0:03:35.019,0:03:36.499
contact recycling
0:03:36.499,0:03:38.519
this will come in handy in a little bit
0:03:38.519,0:03:45.519
after a little while level couple types[br]of dna_ going on
0:03:45.679,0:03:48.499
the people non
0:03:48.499,0:03:54.129
but will come from it
0:03:54.129,0:03:56.929
you know
0:03:56.929,0:04:01.219
guardsmen classmates that just caused a[br]mom
0:04:01.219,0:04:07.409
at the
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and seat
0:04:10.499,0:04:15.169
thing about these guys is that they'll[br]actually die if you bad detect recycling
0:04:15.169,0:04:19.549
antibiotic which we talked about bailey[br]it to them
0:04:19.549,0:04:24.020
webinar invite you to get the class and[br]inside a vehicle and with that because
0:04:24.020,0:04:27.479
all the cells down and then quickly he[br]did them up
0:04:27.479,0:04:29.270
creating little holes
0:04:29.270,0:04:30.860
and the cell membrane
0:04:30.860,0:04:36.009
then the president's concert through
0:04:36.009,0:04:37.509
and get into the south
0:04:37.509,0:04:40.380
these are the kind of bacteria that we[br]have now
0:04:40.380,0:04:41.360
bacteria
0:04:41.360,0:04:45.570
that got the practically
0:04:45.570,0:04:48.219
but you also get bacteria that cut
0:04:48.219,0:04:49.629
the president's that
0:04:49.629,0:04:50.569
we get one
0:04:50.569,0:04:51.770
say
0:04:51.770,0:04:52.680
this one with
0:04:52.680,0:04:54.889
the wrong piece of dna_ way
0:04:54.889,0:04:58.990
this i was just a plane parts that[br]without any frightening
0:04:58.990,0:05:01.029
more than likely will
0:05:01.029,0:05:04.569
get ones that just didn't pick up any of[br]the president's no end in eighteen x
0:05:04.569,0:05:08.619
display t_-cells on some tight recycling[br]on their plates which is basically some
0:05:08.619,0:05:11.289
fancy jello that has antibiotic in it
0:05:11.289,0:05:14.679
so these guys won't be able to grow
0:05:14.679,0:05:16.910
but these guys well
0:05:16.910,0:05:17.960
plated
0:05:17.960,0:05:20.229
people live
0:05:20.229,0:05:22.370
transformed this tiny plasmids
0:05:22.370,0:05:25.500
onto this plate which has kept recycling[br]it
0:05:25.500,0:05:28.990
have likened that what they're saying[br]they need to be here overnight and allow
0:05:28.990,0:05:30.369
our bacteria to grow
0:05:30.369,0:05:37.369
watered down in a game in the morning
0:05:37.419,0:05:39.020
said he didn't have
0:05:39.020,0:05:42.520
definitely got some colonies regularly[br]remain
0:05:42.520,0:05:44.750
cell not a problem that we have
0:05:44.750,0:05:49.419
is we need everyone distinguish that[br]actually had them at these primates
0:05:49.419,0:05:50.729
from the one
0:05:50.729,0:05:52.029
that night
0:05:52.029,0:05:55.009
vis a man who don't have an idea demand
0:05:55.009,0:05:56.509
resulting famine deal
0:05:56.509,0:06:00.360
this is the same techniques that we did[br]at the very beginning of this
0:06:00.360,0:06:02.009
maintenance enemies back
0:06:02.009,0:06:05.169
smith has been spent on these bacteria
0:06:05.169,0:06:10.650
then reading is the same restriction[br]enzymes
0:06:10.650,0:06:12.490
you might be able to notice
0:06:12.490,0:06:14.210
dot proponents say
0:06:14.210,0:06:18.729
is a different sizes then that means[br]that comes in the background
0:06:18.729,0:06:22.630
surrounding areas isn't something larger[br]belmont
0:06:22.630,0:06:27.320
needs a technique adela electrophoresis[br]and let's get it is it's gonna separate
0:06:27.320,0:06:29.809
are different pieces of dna_ half
0:06:29.809,0:06:32.969
and from this experiment will be able to[br]tell
0:06:32.969,0:06:35.600
the president's that took up the[br]frightening
0:06:35.600,0:06:37.169
from the ones that did it
0:06:37.169,0:06:44.169
bought from the ones that just closed up[br]on themselves
0:06:44.639,0:06:47.710
deflating techniques that i don't know
0:06:47.710,0:06:51.460
feel their lives
0:06:51.460,0:06:54.179
galina being made
0:06:54.179,0:06:56.110
very faint
0:06:56.110,0:06:59.499
well i thought that i printed a gag[br]thanks for stopping by
0:06:59.499,0:07:03.269
of the american aircon she'd seen or[br]some of the cloning techniques he's not
0:07:03.269,0:07:04.490
today
0:07:04.490,0:07:07.150
disheveled and they say the internet[br]engineering
0:07:07.150,0:07:14.129
ta waiters
0:07:14.129,0:07:15.069
k