[Script Info]
Title: 
[Events]
Format: Layer, Start, End, Style, Name, MarginL, MarginR, MarginV, Effect, Text
Dialogue: 0,0:00:08.07,0:00:11.83,Default,,0000,0000,0000,,Let's start our journey today\Nby traveling back in time
Dialogue: 0,0:00:11.83,0:00:14.05,Default,,0000,0000,0000,,to the Stone Age, around 10,000 B.C.
Dialogue: 0,0:00:14.05,0:00:19.38,Default,,0000,0000,0000,,If you don't know what period I'm talking\Nabout, just think of the Flintstones.
Dialogue: 0,0:00:19.38,0:00:24.55,Default,,0000,0000,0000,,It is caveman Fred and his wife Wilma\Nthat started the technological revolution.
Dialogue: 0,0:00:24.55,0:00:27.33,Default,,0000,0000,0000,,Farming, most noticeably\Nthe farming of wheat.
Dialogue: 0,0:00:27.86,0:00:31.85,Default,,0000,0000,0000,,Through the ages, the cultivation \Nand consumption of wheat increases
Dialogue: 0,0:00:31.85,0:00:34.13,Default,,0000,0000,0000,,and is spread all over the world.
Dialogue: 0,0:00:34.93,0:00:38.75,Default,,0000,0000,0000,,Now, we fast forward\Nto something like 2008.
Dialogue: 0,0:00:38.95,0:00:43.21,Default,,0000,0000,0000,,There's more awareness\Naround celiac disease and gluten,
Dialogue: 0,0:00:43.21,0:00:46.05,Default,,0000,0000,0000,,which is present\Nin barley, rye, and wheat.
Dialogue: 0,0:00:46.81,0:00:49.90,Default,,0000,0000,0000,,This results in a completely\Nnew revolution,
Dialogue: 0,0:00:50.19,0:00:54.18,Default,,0000,0000,0000,,and this time, gluten intolerance\Nis spread all over the world.
Dialogue: 0,0:00:54.97,0:00:56.29,Default,,0000,0000,0000,,Or so it seems.
Dialogue: 0,0:00:57.42,0:01:01.30,Default,,0000,0000,0000,,The thing is a lot of people think\Nthey are gluten intolerant,
Dialogue: 0,0:01:01.50,0:01:03.80,Default,,0000,0000,0000,,but they don't really know for sure.
Dialogue: 0,0:01:04.80,0:01:08.54,Default,,0000,0000,0000,,This is due to the inadequate\Ndiagnosis of this intolerance.
Dialogue: 0,0:01:09.87,0:01:14.21,Default,,0000,0000,0000,,Currently, the best way to determine\Nif you are gluten intolerant
Dialogue: 0,0:01:14.21,0:01:17.19,Default,,0000,0000,0000,,is to remove gluten from your diet,
Dialogue: 0,0:01:17.19,0:01:19.82,Default,,0000,0000,0000,,and please read with me from the slide,
Dialogue: 0,0:01:19.82,0:01:22.22,Default,,0000,0000,0000,,and see how you feel without it.
Dialogue: 0,0:01:23.38,0:01:29.14,Default,,0000,0000,0000,,Wow! That is some impressive\Nadvanced technological approach.
Dialogue: 0,0:01:29.57,0:01:31.44,Default,,0000,0000,0000,,We can send people into space,
Dialogue: 0,0:01:31.44,0:01:34.67,Default,,0000,0000,0000,,we can move separate atoms\Nunderneath a microscope,
Dialogue: 0,0:01:34.67,0:01:38.46,Default,,0000,0000,0000,,but we're unable to accurately determine\Nif someone is intolerant
Dialogue: 0,0:01:38.46,0:01:42.96,Default,,0000,0000,0000,,to a compound present in breads,\Npizza, cookies, and whatnot.
Dialogue: 0,0:01:44.53,0:01:48.88,Default,,0000,0000,0000,,Now, imagine an approach\Nthat would be worthy of the 21st century.
Dialogue: 0,0:01:49.39,0:01:51.26,Default,,0000,0000,0000,,We go to the supermarket
Dialogue: 0,0:01:51.26,0:01:54.95,Default,,0000,0000,0000,,and make our way to the section\Nwhere they sell medicines and vitamins.
Dialogue: 0,0:01:55.97,0:01:59.16,Default,,0000,0000,0000,,Usually in that section, you can \Nalso find pregnancy tests.
Dialogue: 0,0:02:00.24,0:02:03.59,Default,,0000,0000,0000,,What is easier than buying a small device,
Dialogue: 0,0:02:03.59,0:02:07.81,Default,,0000,0000,0000,,peeing on it, and just\Nreading out the results?
Dialogue: 0,0:02:07.81,0:02:11.62,Default,,0000,0000,0000,,What if you could buy such a test,\Nnot only for gluten intolerance,
Dialogue: 0,0:02:11.62,0:02:14.05,Default,,0000,0000,0000,,but also for cat allergy\Nand other allergies?
Dialogue: 0,0:02:14.94,0:02:18.58,Default,,0000,0000,0000,,What if you could bring a small tester\Nwith you when you go hiking
Dialogue: 0,0:02:18.58,0:02:21.61,Default,,0000,0000,0000,,to test for lyme disease\Nwhen you've been bitten by a tick?
Dialogue: 0,0:02:23.10,0:02:27.24,Default,,0000,0000,0000,,The reality is that currently\Nblood tests are most common
Dialogue: 0,0:02:27.24,0:02:30.15,Default,,0000,0000,0000,,to determine if you have\Nan allergy or a disease.
Dialogue: 0,0:02:30.24,0:02:32.64,Default,,0000,0000,0000,,Maybe some of you have experienced this.
Dialogue: 0,0:02:32.97,0:02:37.60,Default,,0000,0000,0000,,So the first step is to make \Nan appointment and go to the doctor
Dialogue: 0,0:02:37.60,0:02:39.66,Default,,0000,0000,0000,,and have some of your blood collected.
Dialogue: 0,0:02:40.41,0:02:43.56,Default,,0000,0000,0000,,Then they have your blood,\Nand the fun begins.
Dialogue: 0,0:02:43.56,0:02:48.15,Default,,0000,0000,0000,,Your sample goes through a lengthy process\Nwith various analytical steps,
Dialogue: 0,0:02:48.15,0:02:51.03,Default,,0000,0000,0000,,but in the end, some result\Nthat the doctor can interpret.
Dialogue: 0,0:02:52.03,0:02:54.63,Default,,0000,0000,0000,,Finally, the doctor can come and tell you
Dialogue: 0,0:02:54.63,0:03:00.94,Default,,0000,0000,0000,,that you might be a little allergic\Nto almost everything they tested,
Dialogue: 0,0:03:00.94,0:03:03.58,Default,,0000,0000,0000,,as happened to a friend of mine.
Dialogue: 0,0:03:03.58,0:03:07.62,Default,,0000,0000,0000,,So obviously, the current method\Nhas some drawbacks.
Dialogue: 0,0:03:08.36,0:03:11.68,Default,,0000,0000,0000,,Firstly, you need\Nto actually go to the doctor,
Dialogue: 0,0:03:11.68,0:03:14.88,Default,,0000,0000,0000,,which is not that convenient\Nwhen you're in the middle of a hike.
Dialogue: 0,0:03:15.99,0:03:19.39,Default,,0000,0000,0000,,Furthermore, the method \Nalso takes quite a long time.
Dialogue: 0,0:03:19.69,0:03:22.04,Default,,0000,0000,0000,,The tests themselves\Ntake a couple of hours,
Dialogue: 0,0:03:22.05,0:03:25.12,Default,,0000,0000,0000,,and it's usually days\Nuntil you get back your results.
Dialogue: 0,0:03:25.89,0:03:27.57,Default,,0000,0000,0000,,For me, the biggest problem
Dialogue: 0,0:03:27.57,0:03:30.42,Default,,0000,0000,0000,,was that I wasn't allowed\Nto eat anything beforehand,
Dialogue: 0,0:03:30.42,0:03:33.11,Default,,0000,0000,0000,,and I almost passed out\Nbecause I was so hungry.
Dialogue: 0,0:03:34.51,0:03:37.92,Default,,0000,0000,0000,,Some people are uncomfortable\Nwith all these barriers.
Dialogue: 0,0:03:38.03,0:03:41.65,Default,,0000,0000,0000,,Others have their blood tested\Ntoo late or not at all.
Dialogue: 0,0:03:42.50,0:03:47.73,Default,,0000,0000,0000,,Especially in cases such as lyme disease,\Nthis can cause some serious health issues.
Dialogue: 0,0:03:48.15,0:03:51.70,Default,,0000,0000,0000,,If the infection is not detected\Nand treated in time,
Dialogue: 0,0:03:51.70,0:03:55.21,Default,,0000,0000,0000,,you might end up in a wheelchair\Nfor the remainder of your life,
Dialogue: 0,0:03:55.21,0:03:57.58,Default,,0000,0000,0000,,experiencing excruciating joint pain.
Dialogue: 0,0:03:58.48,0:04:02.82,Default,,0000,0000,0000,,Now, I am a student here\Nat the Delft University of Technology,
Dialogue: 0,0:04:02.82,0:04:06.54,Default,,0000,0000,0000,,and I am part of a team participating\Nin a worldwide competition.
Dialogue: 0,0:04:07.19,0:04:11.40,Default,,0000,0000,0000,,And it is our dream to realize\Nthe simple but sophisticated test,
Dialogue: 0,0:04:11.40,0:04:14.28,Default,,0000,0000,0000,,I mentioned before,\Nfor allergies and diseases.
Dialogue: 0,0:04:15.23,0:04:17.77,Default,,0000,0000,0000,,I hope by now you understand
Dialogue: 0,0:04:17.77,0:04:20.43,Default,,0000,0000,0000,,why it is so important\Nto improve the current methods
Dialogue: 0,0:04:20.43,0:04:22.78,Default,,0000,0000,0000,,and that we have your full support.
Dialogue: 0,0:04:23.76,0:04:27.17,Default,,0000,0000,0000,,There is just this tiny\Naspect of our project
Dialogue: 0,0:04:27.17,0:04:29.95,Default,,0000,0000,0000,,that will probably make you\Na little less supportive.
Dialogue: 0,0:04:30.50,0:04:33.90,Default,,0000,0000,0000,,That's the fact that it's based\Non a genetically modified bacterium.
Dialogue: 0,0:04:34.95,0:04:38.27,Default,,0000,0000,0000,,What? Genetic modification?
Dialogue: 0,0:04:38.50,0:04:40.33,Default,,0000,0000,0000,,It's a scary subject,
Dialogue: 0,0:04:40.33,0:04:44.88,Default,,0000,0000,0000,,and people usually freak out a little\Nwhen this topic is mentioned,
Dialogue: 0,0:04:44.88,0:04:47.47,Default,,0000,0000,0000,,or synthetic biology\Nin general is mentioned.
Dialogue: 0,0:04:48.08,0:04:50.91,Default,,0000,0000,0000,,So I'd like to examine\Nit a little further.
Dialogue: 0,0:04:51.92,0:04:58.03,Default,,0000,0000,0000,,Now, as a kid I was, and I still am,\Na very passionate reader.
Dialogue: 0,0:04:58.51,0:05:00.94,Default,,0000,0000,0000,,The Harry Potter books,\NThe Da Vinci Code series,
Dialogue: 0,0:05:01.23,0:05:03.75,Default,,0000,0000,0000,,Steven King thrillers - I read them all.
Dialogue: 0,0:05:04.68,0:05:08.00,Default,,0000,0000,0000,,Sometimes I would get so lost in a story
Dialogue: 0,0:05:08.00,0:05:11.06,Default,,0000,0000,0000,,that I could not shake it off me\Nwhile already in bed.
Dialogue: 0,0:05:11.65,0:05:13.40,Default,,0000,0000,0000,,Then this funny thing happened,
Dialogue: 0,0:05:13.40,0:05:17.51,Default,,0000,0000,0000,,something most of us have experienced\Nat some point in our lives.
Dialogue: 0,0:05:17.93,0:05:20.80,Default,,0000,0000,0000,,You start to imagine\Nmonsters under your bed.
Dialogue: 0,0:05:21.95,0:05:24.43,Default,,0000,0000,0000,,Without an actual cause to be afraid,
Dialogue: 0,0:05:24.43,0:05:27.71,Default,,0000,0000,0000,,your brain comes up\Nwith these very creative, scary thoughts.
Dialogue: 0,0:05:28.56,0:05:33.12,Default,,0000,0000,0000,,Now, we're all adults here, and we can\Nlaugh at these little childish problems.
Dialogue: 0,0:05:33.19,0:05:35.09,Default,,0000,0000,0000,,I mean come on, monsters under the bed?
Dialogue: 0,0:05:35.82,0:05:37.68,Default,,0000,0000,0000,,We've grown over troubles like that.
Dialogue: 0,0:05:39.41,0:05:40.64,Default,,0000,0000,0000,,Or have we?
Dialogue: 0,0:05:42.11,0:05:47.17,Default,,0000,0000,0000,,I think synthetic biology is the adult\Nversion of the monster under the bed.
Dialogue: 0,0:05:48.20,0:05:50.32,Default,,0000,0000,0000,,We read something about it \Nin the newspaper,
Dialogue: 0,0:05:50.32,0:05:55.33,Default,,0000,0000,0000,,and it gives us this uneasy feeling,\Nwithout actually knowing what it is.
Dialogue: 0,0:05:56.42,0:05:59.29,Default,,0000,0000,0000,,Mostly, I think\Nit's a fear of the unknown.
Dialogue: 0,0:06:00.71,0:06:03.43,Default,,0000,0000,0000,,If you go onto the street\Nand ask random passersby
Dialogue: 0,0:06:03.43,0:06:06.82,Default,,0000,0000,0000,,what they know about\Nand think of synthetic biology,
Dialogue: 0,0:06:07.47,0:06:09.77,Default,,0000,0000,0000,,the answers are usually a little vague.
Dialogue: 0,0:06:09.77,0:06:14.38,Default,,0000,0000,0000,,Like, I've heard people calling it\N"manipulation of nature."
Dialogue: 0,0:06:14.92,0:06:18.43,Default,,0000,0000,0000,,This is often mentioned\Nin combination with GMO plants.
Dialogue: 0,0:06:19.36,0:06:24.04,Default,,0000,0000,0000,,It is especially terms like "manipulation"\Nthat have this negative ring to it.
Dialogue: 0,0:06:25.04,0:06:31.35,Default,,0000,0000,0000,,I'd like you to step back from these\Nnegative vibes and get back to the core.
Dialogue: 0,0:06:32.27,0:06:34.82,Default,,0000,0000,0000,,Synthetic biology, what is it?
Dialogue: 0,0:06:35.75,0:06:37.86,Default,,0000,0000,0000,,Now, we live in the 21st century,
Dialogue: 0,0:06:37.86,0:06:40.40,Default,,0000,0000,0000,,so we can just look up\Nthe definition online.
Dialogue: 0,0:06:40.40,0:06:43.87,Default,,0000,0000,0000,,But this just results\Nin some very elaborate sentences
Dialogue: 0,0:06:43.87,0:06:46.40,Default,,0000,0000,0000,,that actually explain very little.
Dialogue: 0,0:06:46.95,0:06:49.77,Default,,0000,0000,0000,,So I'd like to use iGEM as an example.
Dialogue: 0,0:06:51.38,0:06:54.45,Default,,0000,0000,0000,,iGEM is the worldwide competition\NI mentioned before,
Dialogue: 0,0:06:55.05,0:06:58.78,Default,,0000,0000,0000,,and iGEM stands for "International\NGenetically Engineered Machines."
Dialogue: 0,0:07:00.20,0:07:06.49,Default,,0000,0000,0000,,On the iGEM website it says, "For over ten\Nyears, we've been encouraging students
Dialogue: 0,0:07:06.49,0:07:10.43,Default,,0000,0000,0000,,to work together\Nto solve real-world challenges
Dialogue: 0,0:07:10.43,0:07:13.89,Default,,0000,0000,0000,,by building genetically engineered\Nbiological systems
Dialogue: 0,0:07:14.53,0:07:17.50,Default,,0000,0000,0000,,with standard, interchangeable parts."
Dialogue: 0,0:07:18.41,0:07:22.22,Default,,0000,0000,0000,,Now, a core compound of synthetic biology,
Dialogue: 0,0:07:22.22,0:07:25.66,Default,,0000,0000,0000,,actually all of us, is DNA,
Dialogue: 0,0:07:25.66,0:07:27.83,Default,,0000,0000,0000,,the code in ourselves\Nthat forms the blueprint
Dialogue: 0,0:07:27.83,0:07:31.43,Default,,0000,0000,0000,,for what we look like\Nand for all cellular processes.
Dialogue: 0,0:07:32.26,0:07:35.74,Default,,0000,0000,0000,,Some of the techniques we employ\Ninvolve genetic engineering,
Dialogue: 0,0:07:35.74,0:07:38.81,Default,,0000,0000,0000,,which comes down to the cutting\Nand pasting of this DNA.
Dialogue: 0,0:07:39.48,0:07:44.45,Default,,0000,0000,0000,,Additionally, we can also read DNA codes\Nand write DNA sequences ourselves.
Dialogue: 0,0:07:46.07,0:07:49.54,Default,,0000,0000,0000,,However, I can imagine\Nthis to still be a little vague.
Dialogue: 0,0:07:49.75,0:07:54.06,Default,,0000,0000,0000,,So let's discuss the three things\Nthat biological systems are very good at,
Dialogue: 0,0:07:54.32,0:07:58.48,Default,,0000,0000,0000,,which is making, breaking, and detecting.
Dialogue: 0,0:07:59.34,0:08:05.48,Default,,0000,0000,0000,,For example, there was this iGEM team\Nthat focused on creating an alternate way
Dialogue: 0,0:08:05.48,0:08:07.62,Default,,0000,0000,0000,,for the production\Nof an antimalarial drug.
Dialogue: 0,0:08:08.23,0:08:09.69,Default,,0000,0000,0000,,There's making.
Dialogue: 0,0:08:09.86,0:08:12.66,Default,,0000,0000,0000,,Another team focused\Non obtaining a protein
Dialogue: 0,0:08:12.66,0:08:15.33,Default,,0000,0000,0000,,able to efficiently\Nand quickly degrade plastic
Dialogue: 0,0:08:15.33,0:08:18.60,Default,,0000,0000,0000,,to treat, for example,\Nthe plastic soup in the ocean.
Dialogue: 0,0:08:18.60,0:08:19.73,Default,,0000,0000,0000,,Breaking.
Dialogue: 0,0:08:20.08,0:08:25.57,Default,,0000,0000,0000,,Last but not least, we hope\Nthat our simple but sophisticated test
Dialogue: 0,0:08:25.83,0:08:29.62,Default,,0000,0000,0000,,can be used to detect\Nantibiotic-resistant bacteria,
Dialogue: 0,0:08:29.98,0:08:33.45,Default,,0000,0000,0000,,so that the antibiotic usage\Ncan be adapted accordingly.
Dialogue: 0,0:08:34.68,0:08:40.64,Default,,0000,0000,0000,,As you've probably noticed, I am \Na student in the synthetic biology field,
Dialogue: 0,0:08:40.74,0:08:42.97,Default,,0000,0000,0000,,so of course, I think\Nit's going to be useful.
Dialogue: 0,0:08:44.26,0:08:48.95,Default,,0000,0000,0000,,However, I'm also very aware of the risks\Nthat go paired with genetic engineering,
Dialogue: 0,0:08:49.64,0:08:52.28,Default,,0000,0000,0000,,meaning we can actually change DNA.
Dialogue: 0,0:08:53.58,0:08:57.57,Default,,0000,0000,0000,,With the wrong intention,\Nthis can cause some serious health risks.
Dialogue: 0,0:08:58.98,0:09:01.32,Default,,0000,0000,0000,,Let's compare it to a pair of scissors.
Dialogue: 0,0:09:02.20,0:09:05.69,Default,,0000,0000,0000,,As it just so happens,\NI have a pair with me.
Dialogue: 0,0:09:06.58,0:09:11.27,Default,,0000,0000,0000,,As a child, we all learned that we should\Nnot run around while carrying a pair,
Dialogue: 0,0:09:11.27,0:09:13.97,Default,,0000,0000,0000,,and that if we really\Nhave to walk with them,
Dialogue: 0,0:09:13.97,0:09:16.24,Default,,0000,0000,0000,,we should do it like this, right?
Dialogue: 0,0:09:16.67,0:09:20.14,Default,,0000,0000,0000,,And we don't start out\Nby using the real thing, too.
Dialogue: 0,0:09:20.42,0:09:22.93,Default,,0000,0000,0000,,I still remember the struggles
Dialogue: 0,0:09:22.93,0:09:26.48,Default,,0000,0000,0000,,when I tried to cut anything\Nwith blunt, childproof scissors.
Dialogue: 0,0:09:27.60,0:09:31.55,Default,,0000,0000,0000,,My point is a pair of scissors\Ncan be dangerous,
Dialogue: 0,0:09:31.55,0:09:35.96,Default,,0000,0000,0000,,but when they are used responsibly,\Nthey form a very handy, valuable tool.
Dialogue: 0,0:09:36.60,0:09:40.61,Default,,0000,0000,0000,,And the exact same thing\Nis true for synthetic biology.
Dialogue: 0,0:09:42.38,0:09:44.81,Default,,0000,0000,0000,,There is, of course, this one difference.
Dialogue: 0,0:09:45.48,0:09:49.67,Default,,0000,0000,0000,,And that is that it is easier for you\Nto trust me with this pair of scissors
Dialogue: 0,0:09:49.67,0:09:52.74,Default,,0000,0000,0000,,than it is for you to trust\Nme with synthetic biology.
Dialogue: 0,0:09:53.95,0:09:55.22,Default,,0000,0000,0000,,Why?
Dialogue: 0,0:09:56.32,0:10:01.16,Default,,0000,0000,0000,,Well, if I go walking around like this\Nwith my pair of scissors,
Dialogue: 0,0:10:01.62,0:10:03.93,Default,,0000,0000,0000,,you all know I am doing it wrong
Dialogue: 0,0:10:03.93,0:10:06.97,Default,,0000,0000,0000,,because you have been taught\Nhow to carry them as well.
Dialogue: 0,0:10:07.87,0:10:10.50,Default,,0000,0000,0000,,However, in the case of synthetic biology,
Dialogue: 0,0:10:10.50,0:10:14.13,Default,,0000,0000,0000,,it's much more unlikely that you can tell\Nwhen I'm making a mistake.
Dialogue: 0,0:10:14.42,0:10:18.34,Default,,0000,0000,0000,,Most of you are not familiar\Nwith the safety rules I've been taught
Dialogue: 0,0:10:18.34,0:10:21.01,Default,,0000,0000,0000,,to practice synthetic biology responsibly.
Dialogue: 0,0:10:21.91,0:10:26.08,Default,,0000,0000,0000,,But should this stop us\Nfrom curing diseases?
Dialogue: 0,0:10:26.57,0:10:27.94,Default,,0000,0000,0000,,Saving environments?
Dialogue: 0,0:10:29.73,0:10:34.16,Default,,0000,0000,0000,,And generally trying to make\Nthe world a better place?
Dialogue: 0,0:10:35.84,0:10:37.62,Default,,0000,0000,0000,,I hope you agree with me
Dialogue: 0,0:10:37.62,0:10:41.74,Default,,0000,0000,0000,,that synthetic biology\Ncan be of huge benefit to all humankind.
Dialogue: 0,0:10:42.31,0:10:44.22,Default,,0000,0000,0000,,Yes, there are risks,
Dialogue: 0,0:10:44.44,0:10:48.83,Default,,0000,0000,0000,,but as long as it's practiced responsibly,\Nit poses no threat to us.
Dialogue: 0,0:10:49.86,0:10:54.68,Default,,0000,0000,0000,,In that sense, our fear of the unknown\Nmight actually be a bigger threat
Dialogue: 0,0:10:54.68,0:10:56.52,Default,,0000,0000,0000,,than synthetic biology itself.
Dialogue: 0,0:10:57.67,0:11:01.26,Default,,0000,0000,0000,,To quote from Dan Brown's "Inferno,"
Dialogue: 0,0:11:01.26,0:11:05.14,Default,,0000,0000,0000,,"Only one form of contagion\Ntravels faster than a virus.
Dialogue: 0,0:11:06.34,0:11:07.55,Default,,0000,0000,0000,,And that's fear."
Dialogue: 0,0:11:08.97,0:11:11.68,Default,,0000,0000,0000,,So I hope you all sleep well tonight.
Dialogue: 0,0:11:12.40,0:11:15.03,Default,,0000,0000,0000,,The monsters under your bed\Nare just shadows,
Dialogue: 0,0:11:15.03,0:11:17.07,Default,,0000,0000,0000,,and we can shine a light on them.
Dialogue: 0,0:11:17.07,0:11:18.36,Default,,0000,0000,0000,,Thank you.
Dialogue: 0,0:11:18.73,0:11:21.70,Default,,0000,0000,0000,,(Applause)