0:00:01.059,0:00:02.430 This here is a model of a sarcomere. 0:00:03.359,0:00:03.670 A Sarcomere 0:00:03.960,0:00:06.840 is a contractile unit for skeletal muscle fibers. 0:00:07.369,0:00:08.359 This covering 0:00:08.609,0:00:11.310 which is acting as a dust cover, but it actually represents 0:00:11.649,0:00:16.559 the sarcoplasmic reticulum, which is a modified ER which is storing calcium, 0:00:16.569,0:00:18.510 which is crucial for muscle contraction. 0:00:18.700,0:00:23.069 The parts of this you should know. This is the transverse tubule or T tubule. 0:00:23.229,0:00:24.799 These are the terminal cisternae. 0:00:24.930,0:00:28.159 Off to the sides of that, and they make this triad structure. 0:00:28.709,0:00:31.209 OK. So, then you can look at this. This is the actual sarcomere. 0:00:31.540,0:00:31.989 The sarcomere’s 0:00:32.200,0:00:33.009 boundaries 0:00:33.290,0:00:34.169 are shown at 0:00:34.500,0:00:37.380 the left and right. This is the Z discs 0:00:37.560,0:00:39.970 and they're actually shaped like the letter Z. 0:00:40.799,0:00:42.189 OK. So, what a sarcomere is 0:00:42.419,0:00:45.479 creating the striations that you see under a microscope. 0:00:45.490,0:00:47.619 Now, the striations, these light and dark 0:00:47.799,0:00:50.240 bands that the muscle has 0:00:50.349,0:00:53.990 as a result of these thin and thick myofilaments. 0:00:54.509,0:00:57.549 So, this is a thin myofilament containing mostly actine, 0:00:58.139,0:01:00.639 and this is the thick myofilament containing myosine. 0:01:01.709,0:01:05.279 So, what happens is the acting is attached to the Z discs, right? 0:01:05.290,0:01:08.699 You can see that on both sides, the thin myofilament is attached here. 0:01:09.389,0:01:13.580 The thick myofilament is connected here in the middle, this is called the M line. 0:01:14.029,0:01:17.300 And during the muscle contraction, the thick filaments, 0:01:17.309,0:01:20.540 the myosin, walk the actine towards the middle, 0:01:21.110,0:01:23.089 and it happens on both sides simultaneously. 0:01:23.099,0:01:27.220 So, the whole thing shortens the Z discs actually get closer together. 0:01:27.760,0:01:31.699 So, what's creating the striated appearance when you look at it under a microscope? 0:01:32.529,0:01:34.260 And when you look at this area here, 0:01:34.699,0:01:38.209 just this region here will appear light under a microscope. 0:01:38.330,0:01:40.889 That's called an eye band or light band. 0:01:41.419,0:01:44.660 It appears light because it only has thin myofilaments, 0:01:45.739,0:01:48.559 this area from here to here, 0:01:49.459,0:01:53.449 that will appear dark under a microscope. And that's called the A band or dark band. 0:01:53.459,0:01:55.360 The reason why it appears dark is because 0:01:55.370,0:01:57.830 it contains thick myofilaments and they're thicker, 0:01:57.839,0:01:58.440 of course. 0:01:59.010,0:02:01.540 There is a special area right here, 0:02:01.769,0:02:04.440 where there is no overlap with thick and thin. 0:02:04.449,0:02:08.320 You can see just this middle region where it's just thick myofilaments 0:02:08.740,0:02:10.070 That's called the H band. 0:02:10.080,0:02:13.350 And if you actually look at it from up top, it looks like the letter H. 0:02:14.110,0:02:14.610 All right. 0:02:15.589,0:02:19.630 Additionally, there's some accessory proteins here with the thin myofilament. 0:02:19.639,0:02:21.869 You will learn about this in lecture troponin, 0:02:22.240,0:02:24.970 tropomyosin. That's not something we really go over 0:02:25.360,0:02:26.050 in lab. 0:02:26.500,0:02:27.979 But those are the parts of a sarcomere.