0:00:09.000,0:00:13.000
(English captions by Andrea Matsumoto, University of Michigan.)

0:00:15.000,0:00:20.000
These are the items we require in the stool[br]concentration technique.

0:00:20.000,0:00:27.000
We need the stool sample, the fine mesh of[br]pore size three fifty to four fifteen micrometer,

0:00:27.000,0:00:43.000
the fifteen mL centrifuge tube, a glass applicator[br]for emulsifying the stool sample, the beaker

0:00:43.000,0:00:58.000
for the filtrates, the ten percent Formol-saline[br]for mixing the stool, then the diethyl ether.

0:00:58.000,0:01:12.000
Add ten percent Formol-saline to the stool,[br]about a gram of the stool sample.

0:01:12.000,0:01:21.000
Emulsify the stool sample with the applicator.

0:01:21.000,0:01:31.000
Emulsify it very well to get a smooth substance.

0:01:31.000,0:01:53.000
Sift the stool sample through the fine mesh[br]into the beaker.

0:01:53.000,0:01:57.000
Discard the stool sample.

0:01:57.000,0:02:03.000
The debris is discarded under the tap water[br]because it has been disinfected already with

0:02:03.000,0:02:07.000
the Formol.

0:02:07.000,0:02:17.000
Transfer seven mils of stool filtrate into[br]the fifteen mil centrifuge tube.

0:02:17.000,0:02:30.000
Rinse whatever is left in the beaker into[br]the centrifuge tube.

0:02:30.000,0:02:40.000
Add three mils of the diethyl ether to the[br]seven mil of the stool filtrate in the fifteen

0:02:40.000,0:02:47.000
mil centrifuge tube making it to a total of[br]ten mL.

0:02:47.000,0:02:53.000
And we will see two layers: the ether layer[br]and then the Formol-saline layer.

0:02:53.000,0:03:06.000
Cover the centrifuge tube with a lid and mix[br]the two layers very well.

0:03:06.000,0:03:12.000
And thus the ether is to dissolve in the fat,[br]which is present in the stool sample, to release

0:03:12.000,0:03:16.000
the parasites.

0:03:16.000,0:03:30.000
Put it in the centrifuge tube balancing it.

0:03:30.000,0:03:40.000
Centrifuge at thousand five hundred (1500) RPM for[br]two to five minutes.

0:03:40.000,0:03:45.000
Okay take out the centrifuge tube and you[br]will see four layers.

0:03:45.000,0:03:51.000
The first layer is the ether layer, the debris[br]layer, which is the insoluble pad, and then

0:03:51.000,0:04:04.000
Formol-saline layer, and then the sediment[br]which contains the parasite.

0:04:04.000,0:04:17.000
Break through the debris layer and discard[br]the first three layers under the tap water,

0:04:17.000,0:04:22.000
which have already been treated with Formol.

0:04:22.000,0:04:29.000
So the sediment will contain the parasites[br]of the egg or the larvae and then cysts were

0:04:29.000,0:04:36.000
suspended with part of the Formol-saline or[br]saline.

0:04:36.000,0:04:43.000
Use a disposable pipet to transmit it very[br]well and transfer a drop onto the microscope

0:04:43.000,0:04:45.000
slide.

0:04:45.000,0:04:57.000
Cover the drop of smear with the cover slip[br]and examine it under the microscope using

0:04:57.000,0:05:01.000
the times ten objective (10X).

0:05:01.000,0:05:05.000
Low light allows better to see the parasite[br]of the egg, the cyst, and the larvae.