WEBVTT 00:00:00.264 --> 00:00:09.873 [♫] 00:00:09.873 --> 00:00:11.922 Narrator: An international team of forensic experts 00:00:11.922 --> 00:00:15.205 is flying in to the scene of a 90 year old crime 00:00:15.205 --> 00:00:18.285 in a far-flung Siberian forest, 00:00:18.285 --> 00:00:20.618 including a leading forensic anthropologist 00:00:20.618 --> 00:00:23.101 and veteran of the 9/11 investigation, 00:00:23.101 --> 00:00:25.085 Anthony Falsetti. 00:00:25.402 --> 00:00:27.891 Anthony Falsetti: It's really vast out here. 00:00:27.891 --> 00:00:32.156 So desolate and so far away from civilization, 00:00:32.156 --> 00:00:34.770 but that's where two bodies are supposed to be. 00:00:36.035 --> 00:00:38.303 Narrator: It's another case of huge significance, 00:00:38.303 --> 00:00:39.910 especially for Russia. 00:00:41.161 --> 00:00:43.475 The remains of long lost members of its royal family 00:00:43.475 --> 00:00:45.593 may have been found at last, 00:00:45.593 --> 00:00:47.494 but nobody knows for sure. 00:00:50.061 --> 00:00:51.608 The truth of what happened to the Romanovs 00:00:51.608 --> 00:00:54.344 has long been blurred by myth and legend. 00:00:55.369 --> 00:00:58.986 Did the royal line of Russia end in a violent murder 00:00:58.986 --> 00:01:02.808 or did an heir to the throne escape and survive? 00:01:07.488 --> 00:01:10.571 The investigation team also includes Dr. Michael Coble, 00:01:10.571 --> 00:01:12.319 a leading forensic DNA expert 00:01:12.319 --> 00:01:13.919 who works for the Pentagon, 00:01:13.919 --> 00:01:16.918 identifying the remains of American soldiers. 00:01:18.619 --> 00:01:21.635 With the American military's laboratories at his full disposal, 00:01:21.635 --> 00:01:25.488 he'll attempt to put names to these mystery bones. 00:01:27.504 --> 00:01:29.638 The team is headed into a remote forest, 00:01:29.638 --> 00:01:33.502 20 kilometers outside the Siberian city of Yekaterinburg. 00:01:34.620 --> 00:01:40.252 [♫] 00:01:40.252 --> 00:01:42.155 This far-flung industiral outpost 00:01:42.155 --> 00:01:45.173 is where the fate of Russia's royal family was written. 00:01:50.438 --> 00:01:51.837 Five children, 00:01:51.837 --> 00:01:54.022 born into royalty. 00:01:56.120 --> 00:01:59.169 The grand duchesses Olga and Tatiana, 00:01:59.169 --> 00:02:01.608 the flower of young womanhood. 00:02:02.541 --> 00:02:05.969 Maria and Anastasia are beguiling teenagers. 00:02:08.734 --> 00:02:10.567 13 year old Crown Prince Alexei 00:02:10.567 --> 00:02:12.944 is the boy born to be king. 00:02:12.944 --> 00:02:15.073 Millions of loyal Russians revere them, 00:02:15.073 --> 00:02:17.450 even worship them. 00:02:19.349 --> 00:02:22.941 A divine family set on Earth to rule the nation. 00:02:22.941 --> 00:02:26.090 But the Romanov line would end with them. 00:02:29.902 --> 00:02:32.602 1917, the Russian Revolution. 00:02:32.602 --> 00:02:34.817 In its wake, a civil war raged, 00:02:34.817 --> 00:02:37.981 the Bolsheviks against the Czarist loyalists. 00:02:41.665 --> 00:02:42.581 [explosions/gunshots] 00:02:43.429 --> 00:02:45.147 The royal family was imprisoned, 00:02:45.147 --> 00:02:46.663 exiled to Siberia, 00:02:46.663 --> 00:02:49.234 under house arrest in Yekaterinburg. 00:02:51.151 --> 00:02:52.700 But their popularity among the masses 00:02:52.700 --> 00:02:56.456 presented a problem for the fledgling Soviet administration. 00:02:58.689 --> 00:03:00.756 The Bolshevik leadership planned a show trial 00:03:00.756 --> 00:03:03.437 and execution for the Czar. 00:03:04.419 --> 00:03:06.420 Perhaps wary of a Populist backlash, 00:03:06.420 --> 00:03:09.901 some accounts say Lenin wanted the rest of the family kept alive 00:03:09.901 --> 00:03:11.883 as political pawns. 00:03:13.617 --> 00:03:16.221 From here, forensic anthropologist Dr. Anthony Falsetti 00:03:16.221 --> 00:03:17.821 and the investigative team 00:03:17.821 --> 00:03:20.864 have to complete their journey on foot, 00:03:20.864 --> 00:03:25.229 to meet the man who may have made a stunning historical find. 00:03:25.229 --> 00:03:28.119 [indistinct greetings] 00:03:28.119 --> 00:03:30.687 Falsetti: So, what did you find? 00:03:30.687 --> 00:03:32.206 Where did you find it? 00:03:32.206 --> 00:03:35.362 [speaking Russian] 00:03:35.362 --> 00:03:37.043 How deep was the burial? 00:03:37.043 --> 00:03:40.443 Narrator: The remains have been moved to a morgue for safekeeping. 00:03:40.443 --> 00:03:43.043 Falsetti: What else did you find with it, any artifcats? 00:03:43.043 --> 00:03:45.209 [translating into Russian] 00:03:45.209 --> 00:03:46.759 [speaking Russian] 00:03:46.759 --> 00:03:48.840 Falsetti: What I'm hearing from these archeologists 00:03:48.840 --> 00:03:50.823 is they have bones, 00:03:50.823 --> 00:03:53.272 maybe some projectiles. 00:03:53.272 --> 00:03:56.021 What we don't have is any evidence 00:03:56.021 --> 00:03:58.786 of a really controlled excavation, 00:03:58.786 --> 00:04:01.782 and it's quite frankly making me nervous. 00:04:02.632 --> 00:04:04.763 Narrator: So far, there's not much to go on. 00:04:04.763 --> 00:04:06.764 There's no evidence of the crime scene 00:04:06.764 --> 00:04:09.488 and scant documentation to support the find. 00:04:10.172 --> 00:04:12.337 The Romanov case has been plagued by hoaxes 00:04:12.337 --> 00:04:14.421 and coverups over the years 00:04:14.421 --> 00:04:17.585 and this latest find may be no different. 00:04:20.803 --> 00:04:22.266 In the days after the murders, 00:04:22.266 --> 00:04:25.491 newspapers reported only that the Czar had been killed. 00:04:26.992 --> 00:04:29.008 For eight years, the Soviet state maintained 00:04:29.008 --> 00:04:31.906 that the rest of the royal family was alive and well, 00:04:31.906 --> 00:04:34.127 but the coverup failed, 00:04:34.127 --> 00:04:36.824 forcing the Russian government to change its story 00:04:36.824 --> 00:04:39.090 and make a shocking admission. 00:04:40.473 --> 00:04:44.761 All 11 members of the royal household had been executed. 00:04:45.994 --> 00:04:48.893 Then came reported sightings of Prince Alexei 00:04:48.893 --> 00:04:51.655 and Princess Anastasia. 00:04:51.655 --> 00:04:54.974 Were the children dead or alive? 00:04:54.974 --> 00:04:57.875 Sensational rumors kept emerging. 00:05:00.725 --> 00:05:01.784 In Mosow, there was unease 00:05:01.784 --> 00:05:04.520 about the exact fate of the Romanovs. 00:05:05.585 --> 00:05:06.923 An imposter claiming to be Alexei 00:05:06.923 --> 00:05:09.047 was officially investigated. 00:05:09.780 --> 00:05:11.913 Years pass. 00:05:12.849 --> 00:05:14.615 Under Stalin's iron rule, 00:05:14.615 --> 00:05:17.973 it was forbidden to even mention the royal family. 00:05:19.607 --> 00:05:22.040 In the 1950s, a member of the original firing squad 00:05:22.040 --> 00:05:24.305 surfaced in the United States. 00:05:24.305 --> 00:05:25.836 Austrian Rudolf Lacher 00:05:25.836 --> 00:05:27.568 claimed he had been left to guard a truck 00:05:27.568 --> 00:05:29.727 carrying the royal bodies. 00:05:29.727 --> 00:05:32.044 When it got stuck in the mud in the middle of a forest, 00:05:32.044 --> 00:05:35.784 he said he helped a wounded Anastasia escape. 00:05:37.038 --> 00:05:42.041 [pained breathing] 00:05:42.041 --> 00:05:44.923 The Romanov legend and rumors of a surviving heir 00:05:44.923 --> 00:05:47.047 were revived. 00:05:51.292 --> 00:05:54.322 After the Soviet Union collapsed in 1991, 00:05:54.322 --> 00:05:57.222 local academics armed with shovels and vital information 00:05:57.222 --> 00:06:00.306 ventured into the Koptyaki Forest to dig. 00:06:04.521 --> 00:06:07.103 Although the Romanovs executioners were long dead, 00:06:07.103 --> 00:06:10.002 papers handed down by the commander, Yakov Yurovsky, 00:06:10.002 --> 00:06:13.685 held clues as to where the bodies of the royal household might lie. 00:06:17.613 --> 00:06:19.844 The dig revealed a shallow grave, 00:06:19.844 --> 00:06:22.577 skulls, bones, full skeletons, 00:06:22.577 --> 00:06:24.593 but something was missing. 00:06:29.682 --> 00:06:33.679 Peter Sarandinaki: In 1991, nine sets of remains were found. 00:06:33.679 --> 00:06:36.669 There were 11 people that were killed 00:06:36.669 --> 00:06:39.434 in Yekaterinburg that night. 00:06:39.434 --> 00:06:42.454 Two sets of remains were still missing. 00:06:43.436 --> 00:06:44.631 Narrator: The Czar and Czarina, 00:06:44.631 --> 00:06:45.623 three of their daughters, 00:06:45.623 --> 00:06:48.073 and four attendants are identified, 00:06:48.073 --> 00:06:49.827 but two of the youngest royal children 00:06:49.827 --> 00:06:52.527 aren't among the dead. 00:06:56.575 --> 00:06:59.592 Now, little more than 60 meters from the first grave, 00:06:59.592 --> 00:07:01.574 a second find is being investigated 00:07:01.574 --> 00:07:04.590 by leading American forensic experts. 00:07:06.757 --> 00:07:09.457 Locked away in a Yekaterinburg city morgue, 00:07:09.457 --> 00:07:10.767 the newly uncovered bones 00:07:10.767 --> 00:07:12.851 will be subject to the most intensive 00:07:12.851 --> 00:07:17.034 21st century forensic analysis. 00:07:17.034 --> 00:07:27.137 [♫] 00:07:27.137 --> 00:07:29.987 Did all of the Russian royals come to a violent end 00:07:29.987 --> 00:07:32.787 at the hands of Bolshevik executioners? 00:07:33.990 --> 00:07:35.970 Or could the legends be true? 00:07:37.505 --> 00:07:41.394 Did two of Czar Nicholas's children survive? 00:07:42.568 --> 00:07:44.536 Forensic anthropologist Anthony Falsetti 00:07:44.536 --> 00:07:46.619 and DNA expert Michael Coble 00:07:46.619 --> 00:07:48.496 are about to get their first look at the bones 00:07:48.496 --> 00:07:50.513 that may answer these intriuging questions 00:07:50.513 --> 00:07:52.647 once and for all. 00:07:53.713 --> 00:07:56.080 Under lock and key in the Yekaterinburg morgue, 00:07:56.080 --> 00:07:58.328 access to these potentially royal remains 00:07:58.328 --> 00:08:00.327 is tightly controlled. 00:08:02.293 --> 00:08:05.375 In the year 2000, the Romanovs were canonized as martyrs 00:08:05.375 --> 00:08:07.531 by the Russian Orthodox Church. 00:08:07.531 --> 00:08:11.964 If authenticated, these bones could become holy relics. 00:08:17.512 --> 00:08:20.649 Anthony Falsetti is on stranger to these halls. 00:08:20.649 --> 00:08:21.999 He was part of the original team 00:08:21.999 --> 00:08:23.496 assembled in the 1990s 00:08:23.496 --> 00:08:26.631 to investigate the first set of Romanov bones. 00:08:29.946 --> 00:08:33.528 The investigation and its findings proved highly controversial, 00:08:33.528 --> 00:08:36.079 sparking a very public row. 00:08:36.894 --> 00:08:42.513 [indistinct speech] 00:08:42.513 --> 00:08:45.612 Russian scientists used facial reconstruction techniques 00:08:45.612 --> 00:08:48.912 to claim that one of the bodes was that of Anastasia, 00:08:48.912 --> 00:08:51.406 but others were unconvinced. 00:08:51.406 --> 00:08:54.196 Still more troubling was the body count discrepancy. 00:08:54.196 --> 00:08:55.891 Man: Two bodies are still missing 00:08:55.891 --> 00:08:57.397 and it is a mystery. 00:08:57.397 --> 00:08:58.747 Narrator: If two bodies were missing, 00:08:58.747 --> 00:09:02.070 was the entire grave unrelated to the Romanovs? 00:09:02.070 --> 00:09:04.782 There were accusations of political interference 00:09:04.782 --> 00:09:07.331 and rushed, rash conclusions. 00:09:08.681 --> 00:09:10.230 In the end, the Russian Orthodox Church 00:09:10.230 --> 00:09:12.979 rejected the DNA evidence as tainted 00:09:12.979 --> 00:09:14.791 and refused to acknowledge that the remains 00:09:14.791 --> 00:09:17.074 were in fact the Romanovs. 00:09:21.305 --> 00:09:24.489 But now this new find could change all that, 00:09:24.489 --> 00:09:27.637 and this time the team wants to avoid the storm of publicity 00:09:27.637 --> 00:09:31.068 that engulfed investigations in 1991. 00:09:31.068 --> 00:09:34.296 But the Romanov mystery is an enduring fascination. 00:09:34.296 --> 00:09:38.551 [♫] 00:09:38.551 --> 00:09:42.789 Man: We arrive into the lab and there's a massive number of cameras 00:09:42.789 --> 00:09:45.289 and people doing interviews 00:09:45.289 --> 00:09:48.709 while we're trying to look at the remains. 00:09:48.709 --> 00:09:51.794 Narrator: The start of the latest investigation makes the evening news. 00:09:51.794 --> 00:09:55.610 [speaking Russian] 00:09:55.610 --> 00:09:57.307 But once the cameras have gone, 00:09:57.307 --> 00:09:59.575 a critical story emerges. 00:10:01.656 --> 00:10:03.030 Falsetti: Once the cameras were gone, 00:10:03.030 --> 00:10:04.963 we get down to work 00:10:04.963 --> 00:10:07.080 and what I discover is that 00:10:07.080 --> 00:10:09.128 these 44 fragments, 00:10:09.128 --> 00:10:13.290 many we're not going to be able to identify as being human. 00:10:13.290 --> 00:10:15.394 Perhaps they're not. 00:10:15.394 --> 00:10:18.316 There's just not enough material here. 00:10:19.016 --> 00:10:19.931 Narrator: These shattered remains 00:10:19.931 --> 00:10:22.015 look nothing like the nearly complete skeletons 00:10:22.015 --> 00:10:24.072 found in 1991. 00:10:24.072 --> 00:10:26.980 Broken, almost unrecognizable. 00:10:31.696 --> 00:10:33.579 But careful inspection by expert eyes 00:10:33.579 --> 00:10:37.329 reveals the fragmentary remains are human. 00:10:39.445 --> 00:10:41.554 Man: Two people. 00:10:41.554 --> 00:10:44.188 Narrator: But exactly who are they the remains of? 00:10:44.188 --> 00:10:48.635 Could these be the bones of Alexei, Maria, or Anastasia, 00:10:48.635 --> 00:10:51.251 or do they belong to someone else entirely? 00:10:54.819 --> 00:10:55.881 Falsetti: The mystery within the mystery 00:10:55.881 --> 00:10:58.281 is what happened to Anastasia? 00:10:58.281 --> 00:10:59.281 Did she escape? 00:10:59.281 --> 00:11:01.686 Or, is she here? 00:11:03.318 --> 00:11:06.934 Right now, I can't tell whether I've two females 00:11:06.934 --> 00:11:09.881 or a male and a female, 00:11:09.881 --> 00:11:11.798 or whether these fragments 00:11:11.798 --> 00:11:16.002 are part of the other bones that were already recovered. 00:11:19.633 --> 00:11:22.482 Narrator: Handling and photographing what may be the bones of saints 00:11:22.482 --> 00:11:24.259 is a delicate matter. 00:11:24.259 --> 00:11:25.692 Each fragment must be handled 00:11:25.692 --> 00:11:28.742 with the greatest respect and sensitivity. 00:11:28.742 --> 00:11:35.069 [♫] 00:11:35.069 --> 00:11:38.069 Then, after intricate examination, 00:11:38.069 --> 00:11:39.784 a breakthrough. 00:11:40.353 --> 00:11:42.047 Falsetti: It doesn't look like much. 00:11:42.047 --> 00:11:45.414 It is a portion of the pelvis. 00:11:45.414 --> 00:11:47.696 It is our os-cox. 00:11:47.696 --> 00:11:49.313 And, as it turns out, 00:11:49.313 --> 00:11:51.199 it is from a female. 00:11:51.199 --> 00:11:55.165 We can deduce that by the sciatic notch. 00:11:55.165 --> 00:11:57.647 In males, it would be more narrow. 00:11:57.647 --> 00:11:59.730 In females, 00:11:59.730 --> 00:12:01.495 it is quite wide. 00:12:01.495 --> 00:12:02.631 That is critical. 00:12:02.631 --> 00:12:04.766 We know we have two people. 00:12:04.766 --> 00:12:08.065 Now we know that one of them is female. 00:12:08.353 --> 00:12:11.347 Narrator: A male and a female. 00:12:11.347 --> 00:12:13.613 Body parts from two individuals. 00:12:13.613 --> 00:12:16.703 But are they the missing Romanov children? 00:12:17.303 --> 00:12:19.542 Anthony Falsetti and the forensic anthropologists 00:12:19.542 --> 00:12:22.009 will have to dig deeper. 00:12:24.491 --> 00:12:26.275 For Mike Coble and the DNA team, 00:12:26.275 --> 00:12:27.934 there's another problem. 00:12:27.934 --> 00:12:30.831 These bones show evidence of burning. 00:12:32.699 --> 00:12:34.730 Michael Coble: Looking at the remains, 00:12:34.730 --> 00:12:35.929 I'm beginning to think 00:12:35.929 --> 00:12:38.329 this is going to be a very difficult case. 00:12:38.329 --> 00:12:39.930 When, when the bone is burned, 00:12:39.930 --> 00:12:41.611 there's a lot of heat generated 00:12:41.611 --> 00:12:43.617 which is not very good for DNA. 00:12:43.617 --> 00:12:46.466 Take our sample from down here. 00:12:46.466 --> 00:12:48.671 2.5, 3 centimeter cut. 00:12:48.671 --> 00:12:50.172 Narrator: Michael Coble selects fragments 00:12:50.172 --> 00:12:53.304 most likely to yield readable DNA. 00:12:54.273 --> 00:12:57.888 Minute pieces will be cut off and sent to his lab in America 00:12:57.888 --> 00:13:00.030 and to other researchers around the world 00:13:00.030 --> 00:13:01.606 for independent analysis. 00:13:01.606 --> 00:13:03.118 Coble: These are the fragments that we think... 00:13:03.118 --> 00:13:04.873 Narrator: But teasing out the 90 year old genes 00:13:04.873 --> 00:13:06.584 that may lie dormant in the fragments 00:13:06.584 --> 00:13:08.166 will take weeks 00:13:08.166 --> 00:13:10.783 and the chances that the DNA has survived intact 00:13:10.783 --> 00:13:12.616 are slim. 00:13:12.616 --> 00:13:19.651 [♫] 00:13:19.651 --> 00:13:20.665 For the forensic team, 00:13:20.665 --> 00:13:22.579 a few pieces of badly damaged bone 00:13:22.579 --> 00:13:24.921 isn't much to go on. 00:13:26.421 --> 00:13:29.004 But, in the town where the Romanov family met their end, 00:13:29.004 --> 00:13:31.554 there are more leads to pursue. 00:13:31.554 --> 00:13:38.402 [♫] 00:13:38.402 --> 00:13:40.551 Local archeologist Sergei Prokofiev 00:13:40.551 --> 00:13:41.635 was one of the excavators 00:13:41.635 --> 00:13:44.863 who found the second lot of bones in 2007. 00:13:46.377 --> 00:13:48.260 He has kept and carefully stored evidence 00:13:48.260 --> 00:13:50.399 taken from the grave in the forest. 00:13:51.051 --> 00:13:52.630 Among the artifacts he's preserved 00:13:52.630 --> 00:13:55.332 are fragments of a wooden crate, 00:13:55.332 --> 00:13:56.782 quantities of ash, 00:13:56.782 --> 00:13:58.846 and some pieces of pottery. 00:13:59.580 --> 00:14:01.329 Each is a clue to what might have happened 00:14:01.329 --> 00:14:03.137 when the remains were buried. 00:14:03.137 --> 00:14:09.619 [♫] 00:14:09.619 --> 00:14:11.501 The ash corroborates with burn marks 00:14:11.501 --> 00:14:13.391 found on the bones. 00:14:14.757 --> 00:14:17.587 A grim picture is beginning to materialize. 00:14:17.587 --> 00:14:19.086 Falsetti: What kind of container... 00:14:22.352 --> 00:14:23.012 [Grunt] 00:14:23.012 --> 00:14:26.590 Narrator: The pottery shards are perhaps the most compelling evidence. 00:14:26.590 --> 00:14:29.125 They match similar fragments found in the nearby grave 00:14:29.125 --> 00:14:31.715 uncovered in 1991. 00:14:31.715 --> 00:14:32.996 Falsetti: These are the ones from 1991? 00:14:32.996 --> 00:14:36.045 Sergei Prokofiev: Da [continues in Russian] 00:14:36.045 --> 00:14:37.362 Narrator: Other pieces of the puzzle 00:14:37.362 --> 00:14:39.962 will have to be found and put together correctly 00:14:39.962 --> 00:14:42.376 before a true picture can emerge 00:14:42.376 --> 00:14:44.278 of exactly what happened in the forest 00:14:44.278 --> 00:14:48.059 outside Yekaterinburg more than 90 years ago. 00:14:48.059 --> 00:14:52.099 [indistinct speech] 00:14:52.099 --> 00:14:55.309 The most important pieces to that puzzle: 00:14:56.925 --> 00:14:58.825 three bullets. 00:14:59.791 --> 00:15:01.037 Exhumed from the grave, 00:15:01.037 --> 00:15:02.836 these projectiles may be the very bullets 00:15:02.836 --> 00:15:06.190 that killed the heir to the Romanov throne. 00:15:06.190 --> 00:15:07.640 [gunshot] 00:15:08.313 --> 00:15:11.963 If they match the bullets found in the nearby grave in 1991, 00:15:11.963 --> 00:15:14.478 a conclusive forensic connection could be made 00:15:14.478 --> 00:15:17.277 between the two graves. 00:15:17.277 --> 00:15:25.710 [♫] 00:15:25.710 --> 00:15:28.700 The bullets are taken for expert analysis. 00:15:30.633 --> 00:15:32.083 When fired from a gun, 00:15:32.083 --> 00:15:33.982 a bullet is scored with distinctive scratches 00:15:33.982 --> 00:15:36.429 as it travels through the barrel. 00:15:38.578 --> 00:15:42.148 These so-called ball markings are like fingerprints. 00:15:45.915 --> 00:15:49.496 The first of the three bullets found in the 2007 grave excavation 00:15:49.496 --> 00:15:52.760 is too damaged for analysis, 00:15:53.794 --> 00:15:56.976 but the other two have survived intact. 00:15:59.176 --> 00:16:03.791 Initial tests show the other two bullets have nearly identical ball marks, 00:16:03.791 --> 00:16:07.140 implying that the same kind of gun was used. 00:16:08.025 --> 00:16:09.449 Tech [interpreted from Russian]: We can determine that the bullets 00:16:09.449 --> 00:16:13.154 were shot from the same weapon, a browning pistol. 00:16:13.154 --> 00:16:15.837 Narrator: The type of weapon may have been identified, 00:16:15.837 --> 00:16:20.797 but the results have exposed yet another twist in the tale. 00:16:20.797 --> 00:16:22.530 Tech [interpreted from Russian]: These bullets are different in caliber 00:16:22.530 --> 00:16:25.137 than the ones found in 1991. 00:16:26.286 --> 00:16:29.935 Narrator: The bullets don't match those found in the earlier grave, 00:16:29.935 --> 00:16:32.284 meaning a different gun had to have been used 00:16:32.284 --> 00:16:35.694 to kill those buried in the other grave nearby. 00:16:36.494 --> 00:16:39.427 Falsetti: We know that this gun is from the same time period, 00:16:39.427 --> 00:16:44.025 but we don't have a direct tie to the '91 finds. 00:16:45.940 --> 00:16:47.399 Narrator: No link. 00:16:47.399 --> 00:16:50.862 A new and previously unknown weapon. 00:16:53.811 --> 00:16:57.428 If there's no hard evidence linking the graves to the same crime, 00:16:57.428 --> 00:17:01.727 then perhaps the remains in the latest grave belong to nameless victims 00:17:01.727 --> 00:17:05.092 and have nothing to do with the slaughter of the Romanov family. 00:17:05.092 --> 00:17:10.625 [♫] 00:17:10.625 --> 00:17:14.522 So far, forensic investigations haven't been able to link the graves 00:17:14.522 --> 00:17:17.286 or the remains found inside them. 00:17:18.918 --> 00:17:24.750 In Russia, the forensic investigator Anthony Falsetti's trail is running cold. 00:17:29.916 --> 00:17:32.015 But the answer to the lost bones' identity 00:17:32.015 --> 00:17:33.947 and to what really happened to Anastasia 00:17:33.947 --> 00:17:35.980 and the rest of the Romanov children 00:17:35.980 --> 00:17:38.563 may be inside this box. 00:17:41.529 --> 00:17:43.946 These are the samples DNA expert Michael Coble 00:17:43.946 --> 00:17:47.127 selected back at the Yekaterinburg Morgue. 00:17:47.127 --> 00:17:50.027 They've made the journey halfway around the world to the United States, 00:17:50.027 --> 00:17:53.452 sealed in contamination-proof containers. 00:17:54.284 --> 00:17:58.982 Other samples are on their way to Austria and other labs around the world. 00:17:58.982 --> 00:18:02.780 [♫] 00:18:02.780 --> 00:18:05.540 The package is brought into a sterile room. 00:18:07.157 --> 00:18:09.107 At each stage, proper procedures are followed 00:18:09.107 --> 00:18:12.788 to make sure that the samples aren't damaged or contaminated. 00:18:17.906 --> 00:18:21.857 The tiny samples inside weigh just a few grams each. 00:18:21.857 --> 00:18:26.298 Are these the remains of Alexei, Maria, or Anastasia Romanov? 00:18:35.562 --> 00:18:39.044 To find out, viable DNA will have to be extracted, 00:18:39.044 --> 00:18:40.895 then traced back through the generations 00:18:40.895 --> 00:18:44.477 to genetic markers shared by a select group of people: 00:18:44.477 --> 00:18:48.077 the intermarried households of European royalty. 00:18:48.077 --> 00:18:50.360 [♫] 00:18:50.360 --> 00:18:52.841 The Romanov children, like the British royal family, 00:18:52.841 --> 00:18:55.521 were descended from Queen Victoria. 00:18:55.521 --> 00:18:57.421 The DNA in this royal bloodline 00:18:57.421 --> 00:19:01.260 bears unique identifying genetic characteristics. 00:19:01.260 --> 00:19:07.329 [♫] 00:19:07.329 --> 00:19:11.358 Michael Coble and his team are more used to dealing with modern remains. 00:19:12.157 --> 00:19:14.674 These bone fragments are nearly a century old, 00:19:14.674 --> 00:19:18.274 and teasing viable DNA out of them won't be easy. 00:19:20.139 --> 00:19:25.138 DNA is fragile and easily damaged by the ravages of time. 00:19:27.005 --> 00:19:29.188 They've endured almost a century below ground 00:19:29.188 --> 00:19:32.721 in a climate of extremes, well below freezing in winter 00:19:32.721 --> 00:19:35.653 and swelteringly hot in summer. 00:19:35.653 --> 00:19:43.435 [♫] 00:19:43.435 --> 00:19:45.384 To extract and test the DNA, 00:19:45.384 --> 00:19:48.135 most of the powdered sample will be used up. 00:19:48.852 --> 00:19:51.186 If this first attempt fails, 00:19:51.186 --> 00:19:53.085 there might not be enough material left over 00:19:53.085 --> 00:19:56.318 for a second definitive DNA analysis, 00:19:58.367 --> 00:20:03.661 and the mystery of Russia's royal grave may never be solved. 00:20:13.809 --> 00:20:15.359 An international effort is underway 00:20:15.359 --> 00:20:20.163 to solve the 90 year old murder mystery of the Russian royal family. 00:20:21.795 --> 00:20:25.195 American forensic anthropologist, Anthony Falsetti, has a lead 00:20:25.195 --> 00:20:27.812 that may help him piece together separate shreds of evidence 00:20:27.812 --> 00:20:29.897 recovered from the crime scene. 00:20:30.713 --> 00:20:34.213 It's a testimony from a long dead witness. 00:20:37.495 --> 00:20:39.861 Years ago, it would have been unthinkable, 00:20:39.861 --> 00:20:41.777 but now an American investigator 00:20:41.777 --> 00:20:45.826 has high level access to Moscow's state archive. 00:20:46.576 --> 00:20:50.175 Falsetti: Here I am, an American scientist, two floors below ground, 00:20:50.175 --> 00:20:53.224 a hundred years worth of communist secrets. 00:20:53.224 --> 00:20:56.368 Narrator: The keeper of those secrets is Dr. Ludmila Levkova. 00:20:59.733 --> 00:21:03.764 Giant blast doors designed to protect the Kremlin's most precious records 00:21:03.764 --> 00:21:06.704 from nuclear attacks swing open. 00:21:08.587 --> 00:21:09.303 Falsetti: We're down here. 00:21:09.303 --> 00:21:13.869 It's 30 feet, walls are two feet thick, and this is Lenin's archive. 00:21:13.869 --> 00:21:17.602 Narrator: If Soviet archives hold the missing pieces to the Romanov puzzle, 00:21:17.602 --> 00:21:19.589 this is where they'll be. 00:21:19.589 --> 00:21:22.721 Falsetti: So, these are Lenin's documents? These originals? 00:21:22.721 --> 00:21:26.114 Ludmila Levkova: Telegrama. [speaking indistinctly] 00:21:26.114 --> 00:21:27.782 Falsetti: This one's from Copenhagen, correct? 00:21:27.782 --> 00:21:28.314 Ludmila Levkova: Yeah. 00:21:28.314 --> 00:21:29.062 Falsetti: Okay. 00:21:29.062 --> 00:21:31.410 Rumor here going that the Czar has been murdered. 00:21:31.410 --> 00:21:33.822 Kindly wire facts. 00:21:33.822 --> 00:21:36.004 Narrator: This inquiry was one of many sent to Lenin 00:21:36.004 --> 00:21:38.604 in the weeks and days before the killing. 00:21:38.604 --> 00:21:39.918 This was his reply. 00:21:39.918 --> 00:21:42.050 Falsetti: "Rumor not true. Czar safe. 00:21:42.050 --> 00:21:45.250 All rumors are only lie of capitalist press." 00:21:45.250 --> 00:21:47.615 And it's signed Lenin. 00:21:51.047 --> 00:21:52.381 Narrator: When Lenin wrote this reply, 00:21:52.381 --> 00:21:56.265 the royal family was alive, asleep. 00:21:56.265 --> 00:21:58.593 But they had only hours left to live. 00:21:59.022 --> 00:22:01.546 By daybreak on the 17th of July, 00:22:01.546 --> 00:22:04.273 the deed had been done. 00:22:04.273 --> 00:22:07.881 But exactly what happened in the cellar is still open to question. 00:22:08.632 --> 00:22:10.896 Only the executioners knew, 00:22:10.896 --> 00:22:14.370 and their statements are on file in another basement. 00:22:15.488 --> 00:22:19.204 The report filed by the head executioner, Commander Yakov Yurovsky, 00:22:19.204 --> 00:22:21.985 is regarded as the most accurate account, 00:22:21.985 --> 00:22:24.000 and makes chilling reading. 00:22:24.000 --> 00:22:26.286 Likova [interpreted from Russian]: Yurovsky said this about 00:22:26.286 --> 00:22:28.649 the execution of the emperor's family. 00:22:29.781 --> 00:22:31.731 They stood along the wall, 00:22:31.731 --> 00:22:34.913 and here he said the following words to them: 00:22:34.913 --> 00:22:38.145 "The Reign of the Romanovs has reached its end. 00:22:38.145 --> 00:22:40.411 Despite the fact that relatives both outside 00:22:40.411 --> 00:22:43.526 and inside the country are trying to liberate them, 00:22:43.526 --> 00:22:48.359 the Euro-Soviet of the workers' deputies has decreed they must be shot." 00:22:48.359 --> 00:22:51.906 [♫] 00:22:51.906 --> 00:22:54.472 Gregg King: When the executioners opened fire, 00:22:54.472 --> 00:22:56.338 there were a number of problems. 00:22:56.338 --> 00:23:00.105 Amongst them, they were in a basement room with stone walls, 00:23:00.105 --> 00:23:04.124 which sent the bullets sort of ricocheting around the room. 00:23:05.375 --> 00:23:07.641 Likova [interpreted from Russian]: The firing, Yurovsky says, 00:23:07.641 --> 00:23:10.140 becomes ever so confused, 00:23:10.140 --> 00:23:12.174 and when this confused fire ended, 00:23:12.174 --> 00:23:15.407 the shooters saw the daughters were still alive. 00:23:16.073 --> 00:23:18.474 They shot the girls, but nothing happened. 00:23:18.474 --> 00:23:20.900 They weren't able to kill them. 00:23:20.900 --> 00:23:23.216 Narrator: Incredibly, eyewitness accounts agree 00:23:23.216 --> 00:23:25.113 that the duchesses seemed to be protected 00:23:25.113 --> 00:23:29.771 by jewel-filled corsets that acted like bulletproof vests. 00:23:33.521 --> 00:23:35.795 Statements by surviving firing squad members 00:23:35.795 --> 00:23:39.195 recorded in the 1960s confirmed this. 00:23:41.578 --> 00:23:44.736 [Recording] It appeared they'd sewn diamonds into their bras, 00:23:44.736 --> 00:23:47.481 a variety of necklaces, pearls, etc. etc. 00:23:47.481 --> 00:23:48.712 Bullets were bouncing off. 00:23:48.712 --> 00:23:50.962 There was somebody, well, so to say, 00:23:50.962 --> 00:23:53.196 as if they are not finally killed. 00:23:53.196 --> 00:23:56.299 This woman, Anastasia. 00:23:56.982 --> 00:23:58.880 Narrator: A botched execution. 00:23:58.880 --> 00:24:01.866 Anastasia may have survived the initial gunfire. 00:24:01.866 --> 00:24:03.415 Could she really have cheated death 00:24:03.415 --> 00:24:05.938 with a gem-laden corset? 00:24:08.221 --> 00:24:09.727 And if that was true, 00:24:09.727 --> 00:24:11.927 then what else might be possible? 00:24:11.927 --> 00:24:14.027 Falsetti: All the accounts go on to say that 00:24:14.027 --> 00:24:18.595 everyone was finally killed with a gunshot wound to the head. 00:24:18.595 --> 00:24:20.329 But what if that's a lie? 00:24:20.329 --> 00:24:22.965 What if there were co-conspirators? 00:24:24.197 --> 00:24:27.213 Narrator: Co-conspirators that could've helped her escape. 00:24:27.213 --> 00:24:30.063 It's another unsubstantiated story. 00:24:30.063 --> 00:24:32.696 [girl crying] 00:24:32.696 --> 00:24:35.396 The forensic investigator decides to stage an experiment, 00:24:35.396 --> 00:24:38.533 to put the first part of the story to the test. 00:24:39.699 --> 00:24:43.146 Falsetti: Could jewels stop a bullet? 00:24:45.312 --> 00:24:46.457 Narrator: Diamonds are the hardest 00:24:46.457 --> 00:24:49.189 naturally occurring substance known to man, 00:24:49.189 --> 00:24:50.438 but aren't practical 00:24:50.438 --> 00:24:52.825 or within the budget of this experiment. 00:24:53.525 --> 00:24:55.347 Scoring a respectable 8 out of 10 00:24:55.347 --> 00:24:57.215 on the gem hardness scale, 00:24:57.215 --> 00:25:00.730 zirconias are a more realistic alternative. 00:25:01.546 --> 00:25:02.690 The replica corset is finished 00:25:02.690 --> 00:25:05.754 with rose quartz and carnelian. 00:25:06.370 --> 00:25:10.052 Bullets will smash into it at 1,000 feet per second. 00:25:10.052 --> 00:25:12.402 Will they be deflected? 00:25:17.951 --> 00:25:20.216 Interior Ministry Colonel Vladimir Solovyov, 00:25:20.216 --> 00:25:23.011 is the leader of the Romanov investigation. 00:25:23.011 --> 00:25:24.343 He's got special permission 00:25:24.343 --> 00:25:29.123 to handle the actual guns fired by Yurovsky and his execution squad. 00:25:31.390 --> 00:25:35.272 Col Solovyov [interpreted from Russian]: Czar Nicholas II was killed with this pistol. 00:25:35.272 --> 00:25:38.754 This second pistol was also in the basement of the Ipatiev House 00:25:38.754 --> 00:25:43.086 and it may have been used to kill other members of the Czar's family. 00:25:43.086 --> 00:25:48.636 This pistol was also used in all the events in the Ipatiev House. 00:25:50.377 --> 00:25:51.829 Falsetti: It's a grotesque feeling, 00:25:51.829 --> 00:25:54.838 to be in the presence, to hold the weapons, 00:25:54.838 --> 00:25:56.967 that killed this family. 00:25:59.150 --> 00:26:01.965 Narrator: The original guns can't be used in the experiment, 00:26:01.965 --> 00:26:04.164 but the investigation has provided duplicates, 00:26:04.164 --> 00:26:05.764 guns from the same era 00:26:05.764 --> 00:26:08.365 but with less iconic value. 00:26:09.431 --> 00:26:11.046 The reconstructed jewel corsets 00:26:11.046 --> 00:26:14.059 are placed on a mannequin. 00:26:15.391 --> 00:26:19.543 For safety, only the shooter is allowed in the room. 00:26:23.191 --> 00:26:25.155 [gunshot] 00:26:28.755 --> 00:26:29.629 [gunshot] 00:26:29.629 --> 00:26:32.021 Could a diamond corset have shielded Anastasia 00:26:32.021 --> 00:26:34.749 from a hail of bullets? 00:26:39.597 --> 00:26:42.113 Falsetti: We had different powered weapons. 00:26:42.113 --> 00:26:44.181 They were fired sequentially, 00:26:44.181 --> 00:26:47.028 and what we have on the first two 00:26:47.028 --> 00:26:48.472 is clearly some fragmentation. 00:26:48.472 --> 00:26:50.622 There was, these projectiles 00:26:50.622 --> 00:26:52.900 did not pass through on these first two. 00:26:52.900 --> 00:26:56.415 There's an impact site here from the second. 00:26:56.415 --> 00:26:58.632 But none of these were what we would consider 00:26:58.632 --> 00:27:01.597 penetrating gunshot wounds. 00:27:01.597 --> 00:27:04.710 This acts like a bulletproof vest. 00:27:06.726 --> 00:27:09.605 Maybe the legend is true. 00:27:11.872 --> 00:27:13.689 Narrator: Perhaps the bones found in the forest 00:27:13.689 --> 00:27:17.454 aren't from people killed in the basement that night after all. 00:27:17.454 --> 00:27:19.586 A gunshot aimed at the heart, 00:27:19.586 --> 00:27:23.452 deflected by jewels sewn beneath the clothes. 00:27:25.052 --> 00:27:27.202 The accounts that bullets ricocheted, 00:27:27.202 --> 00:27:29.116 that Maria or Anastasia and Alexei 00:27:29.116 --> 00:27:31.547 perhaps survived the initial gunfire 00:27:31.547 --> 00:27:33.851 are becoming plausible. 00:27:34.434 --> 00:27:35.858 For almost a century, these accounts have been 00:27:35.858 --> 00:27:40.196 the basis for many spurious claims to the Russian throne. 00:27:47.662 --> 00:27:50.861 Anna Anderson's claim was the most famous. 00:27:50.861 --> 00:27:54.786 She went to her grave claiming to be the last Grand Duchess Anastasia. 00:27:55.702 --> 00:27:57.534 She convinced many people, 00:27:57.534 --> 00:28:00.232 including the family of Peter Sarandinaki 00:28:00.232 --> 00:28:03.383 whose ancestors were part of the Czar's inner circle. 00:28:03.383 --> 00:28:08.382 [♫] 00:28:08.382 --> 00:28:11.465 Peter Sarandinaki: My mother-in-law, to her dying day, 00:28:11.465 --> 00:28:17.534 to her last day believed that Anna Anderson was Anastasia. 00:28:18.268 --> 00:28:22.266 For instance, she had a triangular injury on her foot 00:28:22.266 --> 00:28:25.501 which was probably a bayonet stab. 00:28:25.501 --> 00:28:27.782 She also had the same 00:28:27.782 --> 00:28:30.969 stubbed toe problem as Anastasia. 00:28:32.101 --> 00:28:36.396 She had the same ear as Anastasia. 00:28:37.178 --> 00:28:40.214 There were a lot of similarities between the two. 00:28:42.530 --> 00:28:44.857 Narrator: Despite her persuasive story, 00:28:44.857 --> 00:28:46.573 DNA testing after her death 00:28:46.573 --> 00:28:48.878 revealed her true identity. 00:28:49.677 --> 00:28:51.263 She was not a royal, 00:28:51.263 --> 00:28:54.246 but a former factory worker from Poland. 00:28:58.626 --> 00:29:00.459 But determining whether the latest remains 00:29:00.459 --> 00:29:02.259 are those of the Romanov family 00:29:02.259 --> 00:29:04.942 or simply another case of mistaken identity 00:29:04.942 --> 00:29:07.142 will take time. 00:29:07.142 --> 00:29:08.641 The DNA is so degraded 00:29:08.641 --> 00:29:10.960 that it must be copied or amplified 00:29:10.960 --> 00:29:13.300 to recover and identify any surviving fragments 00:29:13.300 --> 00:29:15.850 of genetic information that remain. 00:29:19.465 --> 00:29:21.800 At his lab outside Washington D.C., 00:29:21.800 --> 00:29:23.898 DNA expert Michael Coble and his team 00:29:23.898 --> 00:29:26.564 are using a process called PCR, 00:29:26.564 --> 00:29:29.710 or Polymerase Chain Reaction. 00:29:30.056 --> 00:29:32.665 By exposing the faint traces of DNA that remain 00:29:32.665 --> 00:29:34.527 to a bacterial enzyme, 00:29:34.527 --> 00:29:36.891 a single piece of DNA can be multiplied, 00:29:36.891 --> 00:29:39.384 generating millions of copies. 00:29:43.340 --> 00:29:45.125 But even after amplification, 00:29:45.125 --> 00:29:47.439 there are no guarantees that any readable DNA 00:29:47.439 --> 00:29:50.819 will emerge to make identification possible. 00:29:50.819 --> 00:29:53.935 For now, the identity of the two individuals in the grave 00:29:53.935 --> 00:29:56.249 remains a mystery. 00:29:56.249 --> 00:30:03.400 [♫] 00:30:03.400 --> 00:30:06.066 While the search for viable DNA continues in America, 00:30:06.066 --> 00:30:09.081 in Russia the search for clues has come to an end. 00:30:16.821 --> 00:30:18.668 Forensic expert Anthony Falsetti 00:30:18.690 --> 00:30:20.689 is going back with more questions than answers 00:30:20.689 --> 00:30:22.206 about the most recent grave. 00:30:23.134 --> 00:30:25.160 In the 1991 excavation, 00:30:25.377 --> 00:30:27.745 nearly intact, full skeletons were found. 00:30:29.062 --> 00:30:30.660 But in the grave uncovered in 2007, 00:30:30.660 --> 00:30:32.410 only fragments were unearthed. 00:30:33.510 --> 00:30:35.176 Why were there so few bones? 00:30:35.176 --> 00:30:38.956 Less than 10% of a full skeleton was found. 00:30:41.489 --> 00:30:42.805 But even these scant remains 00:30:42.805 --> 00:30:44.839 can reveal more than DNA. 00:30:53.381 --> 00:30:56.666 The degraded condition of the most recently discovered bones 00:30:56.666 --> 00:30:58.563 might actually be a clue in itself. 00:31:00.883 --> 00:31:02.928 Anthony Falsetti is about to take another look 00:31:02.937 --> 00:31:04.260 at the information he's collected 00:31:04.260 --> 00:31:06.957 at his laboratory at the University of Florida. 00:31:06.957 --> 00:31:11.508 [♫] 00:31:11.508 --> 00:31:12.575 Drawing on his long experience 00:31:12.575 --> 00:31:14.456 of bringing stories of the dead back to life, 00:31:14.456 --> 00:31:16.606 he's looking for any patterns 00:31:16.606 --> 00:31:18.555 or links with other evidence. 00:31:25.865 --> 00:31:28.435 He'll come through the accounts of the executioners 00:31:28.435 --> 00:31:31.830 and see if the history and bones tell a common story. 00:31:35.869 --> 00:31:38.664 He already knows that this will be a harrowing tale. 00:31:39.846 --> 00:31:40.829 The savagery did not end 00:31:40.829 --> 00:31:42.564 when the shooting stopped. 00:31:49.681 --> 00:31:51.945 Firing squad leader Yakov Yurovsky wrote 00:31:51.945 --> 00:31:53.375 that after the killing was done, 00:31:53.375 --> 00:31:56.809 he feared that Loyalist troops might catch them red-handed. 00:31:56.809 --> 00:32:01.524 The plan was for a quick and clean disposal. 00:32:01.524 --> 00:32:04.356 But as the bodies were being loaded into a truck, 00:32:04.356 --> 00:32:06.241 the plan unraveled. 00:32:06.241 --> 00:32:10.255 King: As they were lifting up one of the bodies, 00:32:10.255 --> 00:32:12.304 she sat up and screamed. 00:32:12.304 --> 00:32:14.205 It was either Maria or Anastasia. 00:32:14.205 --> 00:32:16.754 We know that two of the grand duchesses 00:32:16.754 --> 00:32:18.312 left that room alive. 00:32:20.257 --> 00:32:22.916 Ermakov grabbed a nearby rifle 00:32:22.916 --> 00:32:26.025 and ended up smashing them repeatedly in the face 00:32:26.025 --> 00:32:27.608 until they stopped screaming. 00:32:36.498 --> 00:32:37.978 Narrator: The bodies were then transported 00:32:37.978 --> 00:32:40.123 a dozen miles outside Yekaterinburg, 00:32:40.123 --> 00:32:41.888 to the Koptyaki Forest, 00:32:41.888 --> 00:32:44.870 to an area of abandoned mineworks. 00:32:46.168 --> 00:32:48.452 Falsetti: So here's what we know. 00:32:48.452 --> 00:32:50.902 The bodies were taken into the forest. 00:32:50.902 --> 00:32:55.283 They were thrown down a mineshaft. 00:32:55.283 --> 00:32:58.400 Grenades were thrown after them 00:32:58.400 --> 00:33:00.181 in an attempt to collapse the walls. 00:33:02.801 --> 00:33:05.349 [explosion] 00:33:08.129 --> 00:33:10.073 Narrator: The bodies were only partially hidden. 00:33:10.073 --> 00:33:14.591 As dawn approached, Yurovsky returned to Yekaterinburg 00:33:14.591 --> 00:33:16.224 to report to his superiors. 00:33:19.204 --> 00:33:21.098 Misfortune struck again. 00:33:21.098 --> 00:33:24.339 Yurovsky's men got drunk and bragged to locals, 00:33:24.339 --> 00:33:26.621 telling them how they killed the royals 00:33:26.621 --> 00:33:29.053 and the location of the bodies. 00:33:29.869 --> 00:33:32.085 Their commander insisted on keeping the burial secret. 00:33:32.085 --> 00:33:34.702 The corpses had to be moved. 00:33:38.702 --> 00:33:40.502 Falsetti: The next day, the bodies were retrieved 00:33:40.502 --> 00:33:42.351 and Yurovsky has his men 00:33:42.351 --> 00:33:44.534 looking for a new location. 00:33:46.754 --> 00:33:48.140 Narrator: But after the bodies were exhumed, 00:33:48.140 --> 00:33:50.973 summer rain turned the ground to a gluey mud. 00:33:52.391 --> 00:33:53.922 The truck got stuck. 00:33:53.922 --> 00:33:55.440 The corpses had to be offloaded. 00:34:00.240 --> 00:34:01.819 Commander Yurovsky was exhausted 00:34:01.819 --> 00:34:03.637 and his men became mutinous. 00:34:04.578 --> 00:34:07.409 He decided to try burning two of the bodies. 00:34:07.409 --> 00:34:09.275 To make the process easier, 00:34:09.275 --> 00:34:12.307 his men were ordered to dismember the corpses. 00:34:16.807 --> 00:34:19.289 Physical damage to fragment #147 00:34:19.289 --> 00:34:21.164 supports the dismemberment story. 00:34:22.313 --> 00:34:23.997 The royal remains were allegedly subject 00:34:23.997 --> 00:34:25.295 to another indignity, 00:34:25.295 --> 00:34:27.728 but the evidence wouldn't be visible. 00:34:36.396 --> 00:34:39.343 Yurovsky claimed that his men poured acid over the corpses, 00:34:39.343 --> 00:34:41.708 to disfigure them beyond recognition. 00:34:47.998 --> 00:34:50.746 Chemical composition tests on the bones conducted in Russia 00:34:50.746 --> 00:34:53.592 revealed faint traces of sulfuric acid. 00:34:55.158 --> 00:34:58.441 According to Yurovsky, it was then that his men 00:34:58.441 --> 00:35:00.608 burned what was left of the two bodies. 00:35:22.518 --> 00:35:24.070 It's another element of the story 00:35:24.070 --> 00:35:26.356 that can be put to a forensic test. 00:35:27.296 --> 00:35:29.378 As an alternative to human flesh, 00:35:29.378 --> 00:35:31.729 pork is a good substitute. 00:35:33.162 --> 00:35:34.578 The composition and structure of the meat 00:35:34.578 --> 00:35:36.111 is similar to its human equivalent 00:35:36.111 --> 00:35:39.511 and pigs are often used in forensic comparisons. 00:35:45.381 --> 00:35:46.715 Falsetti: We know that they had 00:35:46.715 --> 00:35:48.397 between and hour and a half and three hours 00:35:48.397 --> 00:35:51.063 to completely consume two human bodies. 00:35:51.063 --> 00:35:54.345 Narrator: Three hours later, 00:35:54.345 --> 00:35:57.279 the pig carcass is charred but still intact. 00:35:57.279 --> 00:35:59.194 Falsetti: What we've demonstrated here 00:35:59.194 --> 00:36:01.511 is it's not possible to consume a body in a fire, 00:36:01.511 --> 00:36:03.327 certainly not one for three hours, 00:36:03.327 --> 00:36:05.227 relatively low temperatures. 00:36:08.837 --> 00:36:10.985 Narrator: Yurovsky's plan to incinerate the corpses 00:36:10.985 --> 00:36:12.243 was more than a failure. 00:36:12.243 --> 00:36:13.876 It was a fiasco. 00:36:15.326 --> 00:36:18.793 Dawn was breaking and there were still nine bodies in the truck. 00:36:18.793 --> 00:36:22.123 A drastic change in tactics was in order. 00:36:22.123 --> 00:36:25.023 He claimed his men dug a second pit, 00:36:25.023 --> 00:36:26.338 further to the west. 00:36:26.338 --> 00:36:29.738 That they threw the remaining nine bodies into the pit, 00:36:29.738 --> 00:36:31.271 poured acid on them, 00:36:31.271 --> 00:36:34.387 and then covered them with planks and soil. 00:36:38.877 --> 00:36:40.140 Falsetti: What we know about history 00:36:40.140 --> 00:36:42.276 is being reflected by the evidence. 00:36:45.644 --> 00:36:48.168 Narrator: But circumstantial evidence will not be enough 00:36:48.168 --> 00:36:50.505 to put the legend of Anastasia's escape to rest. 00:36:52.239 --> 00:36:57.687 To prove that the remains are not part of some elaborate hoax, 00:36:57.687 --> 00:37:01.720 historical accounts must be corroborated by the irrefutable: 00:37:01.720 --> 00:37:04.134 DNA. 00:37:06.237 --> 00:37:10.219 Do these new bodies belong to Russia's royal bloodline? 00:37:13.606 --> 00:37:16.886 By most accounts, Czar Nicholas II and his royal family 00:37:16.886 --> 00:37:18.794 were a close, tight-knit group, 00:37:18.794 --> 00:37:20.347 frequently seen together, 00:37:20.347 --> 00:37:27.230 filmed here on a cruise when the children were still very young. 00:37:33.101 --> 00:37:36.011 Then, playing tennis while on holiday a few years later. 00:37:39.769 --> 00:37:43.300 And, parading together in this 1913 celebration 00:37:43.300 --> 00:37:45.449 of 300 years of Romanov rule, 00:37:45.449 --> 00:37:49.049 starring the nine year old Crown Prince Alexei 00:37:49.049 --> 00:37:56.731 and the four daughters about to bloom into womanhood. 00:37:58.333 --> 00:38:00.563 But were the Romanovs as inseparable in death 00:38:00.563 --> 00:38:02.029 as they were in life? 00:38:03.030 --> 00:38:04.795 DNA evidence gathered from the second 00:38:04.795 --> 00:38:06.527 and most recent grave attributed to them 00:38:06.527 --> 00:38:07.928 will tell the story. 00:38:12.414 --> 00:38:15.112 Five months after first selecting the bone samples for analysis, 00:38:15.112 --> 00:38:18.762 DNA expert Mike Coble is now ready to share his findings. 00:38:19.522 --> 00:38:20.728 Falsetti: Let's see what you've got. 00:38:20.971 --> 00:38:23.328 Coble: The first thing that we should look at is here. 00:38:23.328 --> 00:38:25.593 This is the marker that determines sex. 00:38:26.290 --> 00:38:27.473 And, sample 146, 00:38:27.473 --> 00:38:29.389 there's an XY, 00:38:29.389 --> 00:38:32.003 which means this is a male sample. 00:38:32.003 --> 00:38:32.752 Falsetti: Right. 00:38:32.752 --> 00:38:35.649 Coble: And then 147 has one X peak 00:38:35.649 --> 00:38:38.116 and this is what we see with females. 00:38:40.527 --> 00:38:42.487 Narrator: So far, the DNA only demonstrates 00:38:42.487 --> 00:38:44.515 that there were two bodies in the grave, 00:38:44.515 --> 00:38:46.731 one male, one female. 00:38:52.057 --> 00:38:53.688 Mike Coble's next step 00:38:53.688 --> 00:38:55.171 was to compare the male sample 00:38:55.171 --> 00:38:57.970 with DNA from Czar Nicholas and his wife, Alexandra. 00:38:59.271 --> 00:39:01.520 Coble: So, what we have here on top, 00:39:01.520 --> 00:39:03.686 this is a profile from the Czar. 00:39:03.686 --> 00:39:07.801 And this on the bottom is a profile from the Czarina. 00:39:07.801 --> 00:39:12.084 And here is the male sample from the second grave. 00:39:12.733 --> 00:39:16.250 We expect that this particular profile 00:39:16.250 --> 00:39:20.949 is going to be a combination of the Czar and the Czarina 00:39:20.949 --> 00:39:23.181 if this is truly Alexei. 00:39:24.006 --> 00:39:29.288 This male, got this 25 repeat peak from the Czar 00:39:29.872 --> 00:39:33.853 and this 23 peak was passed to him 00:39:33.853 --> 00:39:35.788 from the Czarina. 00:39:36.604 --> 00:39:38.921 And here is the male sample... 00:39:39.166 --> 00:39:42.085 Narrator: That makes 17 out of 17 possible matches. 00:39:42.399 --> 00:39:43.786 Coble: The evidence is overwhelming. 00:39:43.786 --> 00:39:45.168 Falsetti: Yeah, absolutely. 00:39:45.168 --> 00:39:46.700 That's their son. Coble: Exactly. 00:39:46.700 --> 00:39:48.035 Falsetti: And they had one son, 00:39:48.035 --> 00:39:49.734 and that's Alexei. Coble: Exactly. 00:39:49.734 --> 00:39:50.667 Falsetti: Right. 00:39:50.667 --> 00:39:53.250 Narrator: Finally there's compelling forensic evidence 00:39:53.250 --> 00:39:55.489 that 13 year old Crown Prince Alexei 00:39:55.489 --> 00:39:56.634 was executed, 00:39:56.634 --> 00:39:58.132 along with the rest of his family, 00:39:58.132 --> 00:40:00.332 though not buried with them. 00:40:05.098 --> 00:40:07.464 But what about the female found in the second grave? 00:40:07.464 --> 00:40:10.729 Mike Coble compared Alexei's DNA with hers. 00:40:11.046 --> 00:40:12.845 Coble: What's interesting though 00:40:12.845 --> 00:40:16.329 is that notice, they both share 23. 00:40:16.983 --> 00:40:19.012 They both share this marker, 00:40:19.012 --> 00:40:21.927 which is a 33.2 repeat. 00:40:21.927 --> 00:40:25.710 Narrator: Once again, one genetic marker after another is shared. 00:40:25.710 --> 00:40:28.426 Coble: ...147 has 17 repeats, 00:40:28.426 --> 00:40:30.608 23 repeats... 00:40:30.608 --> 00:40:35.107 Narrator: Leading to another overwhelming statistical probability. 00:40:35.107 --> 00:40:37.205 Coble: It's over a million times more likely 00:40:37.205 --> 00:40:39.889 that these remains are siblings 00:40:39.889 --> 00:40:43.187 than if they were completely unrelated. Falsetti: Okay. 00:40:43.187 --> 00:40:44.587 Coble: Very strong evidence here 00:40:44.587 --> 00:40:46.570 that we have a brother and a sister. 00:40:47.650 --> 00:40:49.369 Yeah. 00:40:53.084 --> 00:40:55.186 Narrator: That sister was either Maria 00:40:55.186 --> 00:40:56.551 or Anastasia. 00:40:57.586 --> 00:41:00.567 But beyond that, the DNA evidence has nothing else to suggest. 00:41:02.058 --> 00:41:03.766 Coble: Of course, there is a limitation here. 00:41:03.766 --> 00:41:04.998 The DNA can't tell us 00:41:04.998 --> 00:41:08.115 exactly which child is Anastasia 00:41:08.115 --> 00:41:09.815 and which child is Maria. 00:41:09.815 --> 00:41:12.080 Falsetti: The bottom line is really it doesn't matter anymore 00:41:12.080 --> 00:41:15.112 because they're all present and accounted for. 00:41:15.112 --> 00:41:15.911 Coble: Yeah. 00:41:20.581 --> 00:41:22.864 Narrator: After almost an entire century, 00:41:22.864 --> 00:41:25.497 the final chapter of the Romanov dynasty 00:41:25.497 --> 00:41:26.813 can be written at last. 00:41:28.380 --> 00:41:31.281 Anastasia, the youngest Romanov daughter, 00:41:31.281 --> 00:41:33.513 did not escape the firing squad. 00:41:35.036 --> 00:41:36.462 Like her mother, 00:41:38.902 --> 00:41:40.412 her father, 00:41:42.342 --> 00:41:43.727 her baby brother, 00:41:46.857 --> 00:41:48.671 and her three older sisters, 00:41:49.361 --> 00:41:52.457 Anastasia was shot to death in July 1918, 00:41:52.457 --> 00:41:55.641 one month after her 17th birthday. 00:41:57.973 --> 00:42:00.406 She was buried along with the others 00:42:00.406 --> 00:42:02.289 among the towering birch trees. 00:42:07.999 --> 00:42:10.821 It was surely not the end any of them expected 00:42:11.976 --> 00:42:13.275 or des-- 00:42:16.240 --> 00:42:17.890 But the DNA evidence makes it clear, 00:42:17.890 --> 00:42:19.591 that's what really happened. 00:42:19.591 --> 00:42:22.739 On the night when Russia's longest lived dynasty 00:42:22.739 --> 00:42:24.023 came to its abrupt 00:42:24.023 --> 00:42:26.756 and bloody end. 00:42:26.756 --> 00:42:47.341 [♫]