YouTube

Got a YouTube account?

New: enable viewer-created translations and captions on your YouTube channel!

Understanding Reverse Transcriptase – Effects on Ct value

Contribute

Add a new language!

Already have subtitles for this video?
Upload them directly.

Get Embed Code
1 Language

The reverse transcription step is one of the greatest sources of variation in RT-qPCR. With SuperScript IV reverse transcriptase, the Ct value can be reduced by as much as 8. This enzyme outperforms wild type reverse transcriptases with better sensitivity at lower target concentrations. And it shortens the reaction time to just 10 minutes.