well
well it was you know as
typical have really interesting development in that
I was looking for something else and and you know it was that
it was a like PCR with the
the possible the the outcome other solution
to hypothetical problem that did not exist and I was working on
trying to sequence single base-pair
you know then whether call snips today single nucleotide polymorphism
with because those were medically important now is trying
to do it with oligonucleotides because Iran
a lab that made them and we had
really improve the efficiency with which we made them earn
the three or four years prior said
we're making we can make mine faster than the company's
cedars I word for could use them had seven people working for me
and I was thinking on you have to cut step down to like about three kids now
we get the salon omitted devices
on tonight arm into increase the demand
for all you can eat I'm so sorry think you know what else can you do with them
and that it's possible to make if
a rapid clinical and safer single
his polymorphism like sickle cell anemia
was a good example people there that
back then maybe you going ok Lanigan you take a sample maybe three weeks later
you know which is monitor agonizing
other you be nice to have been one shift hospital
in you and that let you know and I
I thought oligonucleotides maybe with the answer there were that
I was really home add as
other chemists I still make him and
I really didn't have an appreciation for happy huge dance
human genome compared to say five thousand base pair
plasma that I was using isn't serb model system
and and I was was thinking this method
require couple organically times wanna miss Serbia control what they would be
pointed
right toward each other just like in PCR reaction and
and then I and I was thinking I mean it really love
pics sexe nucleotide triphosphates
mind there's a couple technical problems trying to solve in 10 min
a the whales in and decide to solve it was to
use to like run a sort of sham reaction
first with the oligonucleotides in place in the sample
and then and then I had my break actresses and suffering and again
and because he was going to use a it is
not for two not the idea and for the fight
duplicating the signal but then I realized the side effect to that was
going to be the house pointed
double the the signal if
there were enough like the exit Euclid has available are trying to get readers
if there are any come in with the same
that you are driving a man was right he does not have this in your head you
thinking about
constantly in and it just realized that there is a way the
you don't think in the lab as much you know I do you think
I was spending my weekends opinion this in a little cabin
and I Drive up every Friday night income a concern
and that's really all the nice things but to a half hours
and e sorry your
uydess got some new to me and you can't do anything else
to think as this when I did most to my thinking
usually his the day-to-day life in a laboratory
doesn't allow alive now
with all these letters in your inbox and use your phone ringing in his own these
people have to deal with and 70 have
really that kind I'm
I'll
of