WEBVTT

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well

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well it was you know as

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typical have really interesting
development in that

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I was looking for something else and and
you know it was that

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it was a like PCR with the

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the possible the the outcome other
solution

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to hypothetical problem that did not
exist and I was working on

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trying to sequence single base-pair

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you know then whether call snips today
single nucleotide polymorphism

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with because those were medically
important now is trying

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to do it with oligonucleotides because
Iran

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a lab that made them and we had

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really improve the efficiency with which
we made them earn

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the three or four years prior said

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we're making we can make mine faster
than the company's

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cedars I word for could use them had
seven people working for me

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and I was thinking on you have to cut
step down to like about three kids now

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we get the salon omitted devices

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on tonight arm into increase the demand

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for all you can eat I'm so sorry think
you know what else can you do with them

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and that it's possible to make if

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a rapid clinical and safer single

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his polymorphism like sickle cell anemia

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was a good example people there that

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back then maybe you going ok Lanigan you
take a sample maybe three weeks later

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you know which is monitor agonizing

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other you be nice to have been one shift
hospital

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in you and that let you know and I

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I thought oligonucleotides maybe with
the answer there were that

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I was really home add as

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other chemists I still make him and

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I really didn't have an appreciation for
happy huge dance

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human genome compared to say five
thousand base pair

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plasma that I was using isn't serb model
system

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and and I was was thinking this method

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require couple organically times wanna
miss Serbia control what they would be

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pointed

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right toward each other just like in PCR
reaction and

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and then I and I was thinking I mean it
really love

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pics sexe nucleotide triphosphates

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mind there's a couple technical problems
trying to solve in 10 min

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a the whales in and decide to solve it
was to

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use to like run a sort of sham reaction

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first with the oligonucleotides in place
in the sample

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and then and then I had my break
actresses and suffering and again

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and because he was going to use a it is

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not for two not the idea and for the
fight

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duplicating the signal but then I
realized the side effect to that was

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going to be the house pointed

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double the the signal if

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there were enough like the exit Euclid
has available are trying to get readers

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if there are any come in with the same

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that you are driving a man was right he
does not have this in your head you

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thinking about

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constantly in and it just realized that
there is a way the

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you don't think in the lab as much you
know I do you think

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I was spending my weekends opinion this
in a little cabin

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and I Drive up every Friday night income
a concern

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and that's really all the nice things
but to a half hours

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and e sorry your

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uydess got some new to me and you can't
do anything else

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to think as this when I did most to my
thinking

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usually his the day-to-day life in a
laboratory

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doesn't allow alive now

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with all these letters in your inbox and
use your phone ringing in his own these

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people have to deal with and 70 have

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really that kind I'm

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I'll

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of