well
well it was you know as
typical have really interesting
development in that
I was looking for something else and and
you know it was that
it was a like PCR with the
the possible the the outcome other
solution
to hypothetical problem that did not
exist and I was working on
trying to sequence single base-pair
you know then whether call snips today
single nucleotide polymorphism
with because those were medically
important now is trying
to do it with oligonucleotides because
Iran
a lab that made them and we had
really improve the efficiency with which
we made them earn
the three or four years prior said
we're making we can make mine faster
than the company's
cedars I word for could use them had
seven people working for me
and I was thinking on you have to cut
step down to like about three kids now
we get the salon omitted devices
on tonight arm into increase the demand
for all you can eat I'm so sorry think
you know what else can you do with them
and that it's possible to make if
a rapid clinical and safer single
his polymorphism like sickle cell anemia
was a good example people there that
back then maybe you going ok Lanigan you
take a sample maybe three weeks later
you know which is monitor agonizing
other you be nice to have been one shift
hospital
in you and that let you know and I
I thought oligonucleotides maybe with
the answer there were that
I was really home add as
other chemists I still make him and
I really didn't have an appreciation for
happy huge dance
human genome compared to say five
thousand base pair
plasma that I was using isn't serb model
system
and and I was was thinking this method
require couple organically times wanna
miss Serbia control what they would be
pointed
right toward each other just like in PCR
reaction and
and then I and I was thinking I mean it
really love
pics sexe nucleotide triphosphates
mind there's a couple technical problems
trying to solve in 10 min
a the whales in and decide to solve it
was to
use to like run a sort of sham reaction
first with the oligonucleotides in place
in the sample
and then and then I had my break
actresses and suffering and again
and because he was going to use a it is
not for two not the idea and for the
fight
duplicating the signal but then I
realized the side effect to that was
going to be the house pointed
double the the signal if
there were enough like the exit Euclid
has available are trying to get readers
if there are any come in with the same
that you are driving a man was right he
does not have this in your head you
thinking about
constantly in and it just realized that
there is a way the
you don't think in the lab as much you
know I do you think
I was spending my weekends opinion this
in a little cabin
and I Drive up every Friday night income
a concern
and that's really all the nice things
but to a half hours
and e sorry your
uydess got some new to me and you can't
do anything else
to think as this when I did most to my
thinking
usually his the day-to-day life in a
laboratory
doesn't allow alive now
with all these letters in your inbox and
use your phone ringing in his own these
people have to deal with and 70 have
really that kind I'm
I'll
of