Genetic Engineering

How to isolate and copy a gene.

you may be wondering what your doing at
the front of a bioengineering madam i a

total strangers talking to you right now
but i did talk about some of the on some

experiments that herbert boyer and
stanley commented in the nation

you might be thinking to yourself

uh... the seventies in america when the
dinosaurs lived

boring

well had never gotten a vaccine are

groceries record he started

that complex to some of the experiments
that these two had

halsted setting

where they accept and values that cut
dat label to in three days

continue bacterial dna_

the hanging at the field of genetic
engineering and thanks to all the work

that they did

we have medications for what that means
that protective

you know i exist on the inside

so the first thing that clinton boy it
did was they checked dna_ from driving

headed up

as you might think you guys that's going
on in this to you

here some dna_ from the front
encouraging traits that affect how the

front develops had survived

now there's a piece that dna_

in this genome whose job listing code
for certain proteins that the front uses

to survive

we're gonna cut this peace out using a
restriction enzyme

this is the thing that way discovered
its

kind of like a pair of scissors

cutting the dna_ act

specific points we can get different
pieces

now there are times are different kinds
restriction enzymes and they'll come in

different places in dna_

are and send their resume tuesday is
called echo are one

now this piece but dna_ has more than
one spot

that echo are one can cut as shown by
the dot lines

so we're going to end up with other
pieces of dna_ in addition to the one

we actually want

now that's actually ok because
articulate that probably did

what's going on in the two is really
really discarding after pieces dna_'s

just like it

now here's a tip

plasmid

basically circulating of dna_ that can
exist in bacteria any basically impose

for certain traits that affect how
bacteria look actor civilian anti at our

dna_ from the frog dna_ try
bacterial dna_

we need to cut the president clinton

so we've got to add are echo are one
scissors

and they're going to cut

at the site

you let this reaction proceeds letting
all the ingredients the classmates

restriction enzyme and somewhat
interesting at cnn_ tel all the

plastic cup like that's

now we need at the dna_ please

and allow the pieces to joint

here's what's going on in the to camp

i've got pieces earth

try to dna_

and i had cracked cut classmates like
this

i need to ad

the sky

into this president

and the way and they do that

is the eddie an enzyme called dna_
like case

this enzyme tenet acts like teak and it
fixes the dna_ in six th

into the test name

nine other important thing at that

the present itself is that it resent
marker than a series of situation in

haiti antibiotic

contact recycling

this will come in handy in a little bit

after a little while level couple types
of dna_ going on

the people non

but will come from it

you know

guardsmen classmates that just caused a
mom

at the

and seat

thing about these guys is that they'll
actually die if you bad detect recycling

antibiotic which we talked about bailey
it to them

webinar invite you to get the class and
inside a vehicle and with that because

all the cells down and then quickly he
did them up

creating little holes

and the cell membrane

then the president's concert through

and get into the south

these are the kind of bacteria that we
have now

bacteria

that got the practically

but you also get bacteria that cut

the president's that

we get one

say

this one with

the wrong piece of dna_ way

this i was just a plane parts that
without any frightening

more than likely will

get ones that just didn't pick up any of
the president's no end in eighteen x

display t_-cells on some tight recycling
on their plates which is basically some

fancy jello that has antibiotic in it

so these guys won't be able to grow

but these guys well

plated

people live

transformed this tiny plasmids

onto this plate which has kept recycling
it

have likened that what they're saying
they need to be here overnight and allow

our bacteria to grow

watered down in a game in the morning

said he didn't have

definitely got some colonies regularly
remain

cell not a problem that we have

is we need everyone distinguish that
actually had them at these primates

from the one

that night

vis a man who don't have an idea demand

resulting famine deal

this is the same techniques that we did
at the very beginning of this

maintenance enemies back

smith has been spent on these bacteria

then reading is the same restriction
enzymes

you might be able to notice

dot proponents say

is a different sizes then that means
that comes in the background

surrounding areas isn't something larger
belmont

needs a technique adela electrophoresis
and let's get it is it's gonna separate

are different pieces of dna_ half

and from this experiment will be able to
tell

the president's that took up the
frightening

from the ones that did it

bought from the ones that just closed up
on themselves

deflating techniques that i don't know

feel their lives

galina being made

very faint

well i thought that i printed a gag
thanks for stopping by

of the american aircon she'd seen or
some of the cloning techniques he's not

today

disheveled and they say the internet
engineering

ta waiters

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